FastQCFastQC Report
Thu 26 May 2016
SRR1545083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545083_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2484304
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG81830.32938802980633614No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74670.30056708035731533No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG51940.20907264167348277No Hit
TTGGAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA27350.11009119656853589No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26830.10799805498843942No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26070.10493884806368303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT302.1665237E-645.0000041
AGCCGAC302.1665237E-645.0000044
TCGTTAG1200.045.0000041
ACCGGGC302.1665237E-645.0000044
CGAACCG351.2126839E-745.00000424
CGCGGGT302.1665237E-645.0000044
CAACGCG351.2126839E-745.0000041
GCGAACG351.2126839E-745.0000041
TAAACGA302.1665237E-645.00000433
ATTCGTC302.1665237E-645.00000414
CGCGACC1200.045.00000410
ACTTACG700.045.0000041
ACGGGTA1300.045.0000045
GTAGCGT351.2126839E-745.0000049
ATATCGA302.1665237E-645.00000411
GTATGCG1300.045.0000041
CGCTAAG302.1665237E-645.00000429
ACGGATG351.2126839E-745.0000041
ACGTGTC351.2126839E-745.00000418
CAATCGA302.1665237E-645.00000422
Produced by FastQC (version 0.11.5)