FastQCFastQC Report
Thu 26 May 2016
SRR1545082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545082_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1043987
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30290.29013771244277947No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG28950.27730230357274566No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG14260.1365917391691659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGCG750.045.0000041
AGTCAAG551.8189894E-1245.0000041
CGCTAGG551.8189894E-1245.0000042
GCTACTG551.8189894E-1245.0000041
GATTACG551.8189894E-1245.0000041
CGGGTTG453.8562575E-1045.06
CGGGTCA253.8905586E-545.06
AAGTACG406.8139343E-945.01
TCGTTTG502.1827873E-1145.01
AATCCGG700.045.02
ACAGTAC351.2117926E-745.016
TCGCAAG253.8905586E-545.01
GTCGATG453.8562575E-1045.01
TGCCATT207.03299E-445.037
CACGGGC351.2117926E-745.04
CAACGGT253.8905586E-545.041
CAACGCG453.8562575E-1045.01
AGATAAC207.03299E-445.045
CTATACG406.8139343E-945.01
CGTGAAT453.8562575E-1045.09