##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545082_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1043987
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.953713983028525	31.0	31.0	33.0	30.0	33.0
2	31.272485193781147	31.0	31.0	33.0	30.0	34.0
3	31.478377604318826	31.0	31.0	34.0	30.0	34.0
4	35.32723491767618	37.0	35.0	37.0	33.0	37.0
5	35.216292923187744	37.0	35.0	37.0	33.0	37.0
6	35.29816654805089	37.0	35.0	37.0	32.0	37.0
7	35.567081774006766	36.0	35.0	37.0	35.0	37.0
8	35.61775194518706	37.0	35.0	37.0	35.0	37.0
9	37.53269437263108	39.0	37.0	39.0	35.0	39.0
10	37.0885518689409	39.0	37.0	39.0	33.0	39.0
11	36.61239555664965	39.0	35.0	39.0	32.0	39.0
12	34.89253027097081	35.0	34.0	39.0	31.0	39.0
13	34.39091674513188	35.0	33.0	39.0	27.0	39.0
14	34.91576140315924	35.0	33.0	40.0	27.0	41.0
15	35.436905823539945	35.0	33.0	40.0	30.0	41.0
16	35.72119959348153	35.0	34.0	40.0	32.0	41.0
17	35.63167549021204	35.0	34.0	40.0	31.0	41.0
18	35.78025013721435	36.0	35.0	39.0	31.0	41.0
19	35.61975005435892	36.0	35.0	39.0	31.0	41.0
20	35.30051810989983	35.0	34.0	39.0	31.0	41.0
21	35.18277047511128	35.0	34.0	39.0	30.0	41.0
22	35.00396748235371	35.0	34.0	39.0	30.0	41.0
23	35.00683437629013	35.0	34.0	39.0	30.0	41.0
24	35.104606666558105	35.0	34.0	39.0	31.0	41.0
25	35.0498291645394	35.0	34.0	39.0	30.0	41.0
26	34.852320000153256	35.0	34.0	38.0	30.0	41.0
27	34.83892807094341	35.0	34.0	39.0	30.0	41.0
28	34.97558877648859	35.0	34.0	39.0	30.0	41.0
29	34.99613979867566	35.0	34.0	38.0	30.0	41.0
30	34.9642888273513	36.0	34.0	38.0	30.0	41.0
31	34.647600018007886	35.0	34.0	38.0	29.0	41.0
32	34.49699565224471	35.0	34.0	39.0	29.0	41.0
33	34.272242853598755	35.0	33.0	39.0	27.0	41.0
34	34.120615486591305	35.0	34.0	38.0	27.0	41.0
35	33.925090063382015	35.0	33.0	38.0	27.0	41.0
36	33.61215417433359	35.0	33.0	38.0	23.0	41.0
37	33.62030849043139	35.0	33.0	38.0	24.0	41.0
38	33.75007734770644	35.0	33.0	38.0	26.0	41.0
39	33.78246280844493	35.0	33.0	38.0	25.0	41.0
40	33.40794281921135	35.0	33.0	38.0	23.0	41.0
41	33.56776760630161	35.0	33.0	38.0	23.0	41.0
42	33.56132787094092	35.0	33.0	38.0	23.0	41.0
43	33.52915218292948	35.0	33.0	38.0	24.0	41.0
44	33.52745101232104	35.0	33.0	38.0	23.0	41.0
45	33.609571766698245	35.0	33.0	38.0	24.0	41.0
46	33.64316892834872	35.0	33.0	38.0	25.0	41.0
47	33.593793792451436	35.0	33.0	38.0	24.0	41.0
48	33.66030707278922	35.0	33.0	38.0	24.0	41.0
49	33.7276843485599	35.0	33.0	38.0	24.0	40.0
50	33.519066808303165	35.0	33.0	38.0	24.0	40.0
51	33.21189823244926	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	4.0
14	8.0
15	17.0
16	63.0
17	181.0
18	443.0
19	871.0
20	1715.0
21	2929.0
22	4649.0
23	6939.0
24	10223.0
25	14013.0
26	18127.0
27	21266.0
28	23795.0
29	27522.0
30	33796.0
31	42489.0
32	55947.0
33	76876.0
34	152444.0
35	227165.0
36	50501.0
37	59503.0
38	86561.0
39	125893.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.142233571873977	25.377519068724038	31.853749136722964	12.626498222679016
2	28.734936354571467	27.163077701159118	32.53584575286857	11.56614019140085
3	28.05427653792624	26.145632081625536	33.76612927172465	12.033962108723575
4	25.293226831368585	28.394414872982132	33.80271976566758	12.509638529981695
5	22.30746168295199	32.93115718873894	32.633356545627485	12.128024582681585
6	19.257327916918506	41.98404769408049	29.39624727127828	9.36237711772273
7	88.01852896635685	4.8950801111508095	6.212625252996445	0.8737656694958845
8	90.88580604930905	3.0203441230590036	5.372384905176022	0.721464922455931
9	87.89755044842512	4.685977890529288	6.081397565295353	1.3350740957502345
10	68.41608180944782	16.541681074572768	10.193996668540892	4.8482404474385214
11	66.00264179534803	13.049300422323268	15.238791287630976	5.709266494697731
12	58.87372160764453	20.478607492238886	14.899993965442098	5.747676934674474
13	17.25366312032621	59.85495987976862	15.18994010461816	7.70143689528701
14	8.316099721548257	58.66643933305683	24.032674736371238	8.984786209023675
15	7.033037767711667	22.553154397516444	62.05642407424613	8.357383760525753
16	7.01349729450654	15.092812458392682	60.573072270057004	17.320617977043774
17	7.7122607848565154	15.801250398711861	30.43486173678408	46.051627079647545
18	14.83581692109193	21.61952208217152	47.249822076328535	16.294838920408015
19	37.613590973833965	17.155769181033865	26.137586004423426	19.093053840708745
20	36.50380703974283	19.919692486592268	28.48569953457275	15.090800939092153
21	15.34875434272649	37.67518177908345	29.854682098531875	17.121381779658176
22	18.892093483922693	24.854907197120273	18.100800105748444	38.15219921320859
23	11.658861652491842	37.576234186824166	23.855373678024726	26.909530482659267
24	8.697809455481725	19.207039934405312	60.68753729692037	11.407613313192597
25	7.614941565364319	24.97674779475223	52.3209580195922	15.08735262029125
26	7.135242105505145	47.905290008400485	27.4986182778138	17.46084960828056
27	7.589941254057761	57.33778294174161	24.271853959867315	10.80042184433331
28	6.746348374069792	35.38865905418363	46.56810860671637	11.296883965030217
29	7.139265144106201	25.74802176655456	44.52929011568152	22.58342297365772
30	12.663950796322176	48.91698842993256	26.998133118515845	11.420927655229423
31	36.805151788288555	30.72442472942671	23.42251388187784	9.047909600406902
32	41.087772165745356	20.727078019170737	27.811265849095822	10.373883965988082
33	40.28680433760191	27.37189256188056	17.316211791909286	15.025091308608248
34	18.147735556094087	25.44418656554153	23.287550515475765	33.120527362888616
35	19.510587775518278	22.46436018839315	37.37833900230558	20.646713033782987
36	42.46872805887429	25.899173073994213	20.473722373937605	11.158376493193881
37	14.832081242390949	49.02379052612724	26.030209188428593	10.113919043053219
38	14.450658868357555	47.67262427597279	21.504865482041442	16.371851373628214
39	19.36978142448134	43.32113330913124	24.783641941901575	12.525443324485842
40	31.24157676292904	23.506806119233286	21.97623150479843	23.275385613039244
41	11.673421220762327	22.06358891442135	27.566531000864952	38.69645886395137
42	14.1828394414873	18.791134372362876	24.908164565267576	42.11786162088225
43	15.693777796083669	19.199472790369995	29.020763668513112	36.08598574503322
44	10.016599823561021	31.37050557142953	36.30696550819119	22.305929096818254
45	9.888820454660834	58.872763741310955	18.401857494394086	12.836558309634125
46	17.204428790779964	48.15031221653143	20.124101162179223	14.521157830509384
47	16.366391535526784	29.757267092406327	24.406913112902746	29.46942825916415
48	16.982491161288408	25.935284634770355	43.862232000973194	13.219992202968045
49	22.094719570262846	26.98529770964581	40.142932814297495	10.777049905793847
50	17.671101268502383	48.88126001568985	22.21972112679564	11.227917589012124
51	15.016757871506062	43.46040707403445	22.394435946041476	19.12839910841802
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	836.0
1	1246.5
2	1657.0
3	3746.5
4	5836.0
5	4831.5
6	3827.0
7	4223.5
8	4620.0
9	5017.0
10	5414.0
11	5501.5
12	5589.0
13	5313.5
14	5038.0
15	4631.5
16	4225.0
17	4070.5
18	3916.0
19	3916.0
20	3916.0
21	3740.0
22	3564.0
23	4096.0
24	4628.0
25	5092.5
26	7767.5
27	9978.0
28	14130.0
29	18282.0
30	22446.0
31	26610.0
32	30049.5
33	33489.0
34	36839.5
35	40190.0
36	39066.5
37	37943.0
38	49546.0
39	61149.0
40	97206.0
41	133263.0
42	161417.5
43	189572.0
44	176877.5
45	164183.0
46	134683.0
47	105183.0
48	85970.5
49	66758.0
50	55703.5
51	44649.0
52	34522.5
53	24396.0
54	17082.5
55	9769.0
56	7692.5
57	5616.0
58	4779.5
59	3943.0
60	3612.5
61	3282.0
62	2797.0
63	2312.0
64	1976.5
65	1641.0
66	1353.5
67	1066.0
68	903.0
69	740.0
70	619.5
71	499.0
72	447.5
73	396.0
74	313.0
75	162.0
76	94.0
77	82.0
78	70.0
79	48.5
80	27.0
81	25.0
82	23.0
83	14.0
84	5.0
85	3.5
86	2.0
87	2.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1043987.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.607332550002548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.96316905606598	12.324044386600924
2	6.912026964960852	2.1575660687345763
3	2.73253825837973	1.279428999124117
4	1.5834767325061279	0.9885539179765828
5	1.0919823373196524	0.8521465738638436
6	0.7940990588839627	0.7436260853787637
7	0.6291580919237036	0.6873635697024821
8	0.49135270091550637	0.6134964002024199
9	0.42982530474853736	0.603758382265495
>10	3.4342457142468246	11.165169623561408
>50	1.0064434204690922	11.601952694041657
>100	1.8941560462251439	52.75481897603772
>500	0.035680756960395694	3.5223711189845095
>1k	0.0018455563945032256	0.7057032035254905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3029	0.29013771244277947	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2895	0.27730230357274566	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1426	0.1365917391691659	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019253113305050733	0.0
2	0.0	0.0	0.0	0.05881299288209527	0.0
3	0.0	0.0	0.0	0.08036498538774908	0.0
4	0.0	0.0	0.0	0.1137945204298521	0.0
5	0.0	0.0	0.0	0.1876460147492258	0.0
6	0.0	0.0	0.0	0.25986913630150565	0.0
7	0.0	0.0	0.0	0.30632565348035945	0.0
8	0.0	0.0	0.0	0.4122656699748177	0.0
9	0.0	0.0	0.0	0.4534539223189561	0.0
10	0.0	0.0	0.0	0.5219413651702559	0.0
11	0.0	0.0	0.0	0.6109271475602666	0.0
12	0.0	0.0	0.0	0.6817134696121695	0.0
13	0.0	0.0	0.0	0.7147598581208386	0.0
14	0.0	0.0	0.0	0.7259668942237787	0.0
15	0.0	0.0	0.0	0.7445495010953201	0.0
16	0.0	0.0	0.0	0.7893776455070801	0.0
17	0.0	0.0	0.0	0.84292237355446	0.0
18	0.0	0.0	0.0	0.9283640505102075	0.0
19	0.0	0.0	0.0	0.9692649429542705	0.0
20	0.0	0.0	0.0	1.007579596297655	0.0
21	0.0	0.0	0.0	1.0683083218469196	0.0
22	0.0	0.0	0.0	1.1307612067966364	0.0
23	0.0	0.0	0.0	1.2140955778184976	0.0
24	0.0	0.0	0.0	1.2735790771341022	0.0
25	0.0	0.0	0.0	1.321280820546616	0.0
26	0.0	0.0	0.0	1.373580322360336	0.0
27	0.0	0.0	0.0	1.4204199860726234	0.0
28	0.0	0.0	0.0	1.473294207686494	0.0
29	0.0	0.0	0.0	1.538045971836814	0.0
30	0.0	0.0	0.0	1.627510687393617	0.0
31	0.0	0.0	0.0	1.71323972424944	0.0
32	0.0	0.0	0.0	1.7839302596679845	0.0
33	0.0	0.0	0.0	1.8498314634186057	0.0
34	0.0	0.0	0.0	1.930004875539638	0.0
35	0.0	0.0	0.0	2.068224987475898	0.0
36	0.0	0.0	0.0	2.1578812762994177	0.0
37	0.0	0.0	0.0	2.2445681794888253	0.0
38	0.0	0.0	0.0	2.32704047081046	0.0
39	0.0	0.0	0.0	2.409608548765454	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	75	0.0	45.000004	1
AGTCAAG	55	1.8189894E-12	45.000004	1
CGCTAGG	55	1.8189894E-12	45.000004	2
GCTACTG	55	1.8189894E-12	45.000004	1
GATTACG	55	1.8189894E-12	45.000004	1
CGGGTTG	45	3.8562575E-10	45.0	6
CGGGTCA	25	3.8905586E-5	45.0	6
AAGTACG	40	6.8139343E-9	45.0	1
TCGTTTG	50	2.1827873E-11	45.0	1
AATCCGG	70	0.0	45.0	2
ACAGTAC	35	1.2117926E-7	45.0	16
TCGCAAG	25	3.8905586E-5	45.0	1
GTCGATG	45	3.8562575E-10	45.0	1
TGCCATT	20	7.03299E-4	45.0	37
CACGGGC	35	1.2117926E-7	45.0	4
CAACGGT	25	3.8905586E-5	45.0	41
CAACGCG	45	3.8562575E-10	45.0	1
AGATAAC	20	7.03299E-4	45.0	45
CTATACG	40	6.8139343E-9	45.0	1
CGTGAAT	45	3.8562575E-10	45.0	9
>>END_MODULE