FastQCFastQC Report
Thu 26 May 2016
SRR1545081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545081_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3113929
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95720.30739300735501673No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46920.15067780928852262No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG39950.12829451153189428No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA39150.12572540992424683No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36220.11631607528623807No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG35300.1133616084374435No Hit
TTTGTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA32030.10286040561618456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG750.045.01
TCGCCCG406.8212103E-945.01
CTAGACC207.0351467E-445.09
AACGTAC207.0351467E-445.021
CTATGCG1350.045.01
AATCCCG750.045.01
CTCACGA502.1827873E-1145.024
CGAACTA453.8562575E-1045.014
ATCTAGT502.1827873E-1145.019
CGCGGGT207.0351467E-445.04
CCCTATG502.1827873E-1145.037
ACGTTAC253.8923477E-545.025
GCGAAGT950.045.02
GGCCGTA453.8562575E-1045.016
GGCAACG1500.045.01
CGACCTT207.0351467E-445.044
CCTACCG207.0351467E-445.041
GTTAGCG750.045.01
TCGATTG1750.045.01
ATCGTTC253.8923477E-545.022
Produced by FastQC (version 0.11.5)