##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545081_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3113929
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90878147831887	31.0	31.0	33.0	30.0	33.0
2	31.24172741253895	31.0	31.0	33.0	30.0	34.0
3	31.399471535799307	31.0	31.0	34.0	30.0	34.0
4	35.205236214441626	37.0	35.0	37.0	33.0	37.0
5	35.06127403675549	37.0	35.0	37.0	32.0	37.0
6	35.15420839717283	37.0	35.0	37.0	32.0	37.0
7	35.569850179628375	36.0	35.0	37.0	35.0	37.0
8	35.64577837195389	37.0	35.0	37.0	35.0	37.0
9	37.51328177360499	39.0	37.0	39.0	35.0	39.0
10	37.09185598001753	39.0	37.0	39.0	33.0	39.0
11	36.60476876640411	39.0	35.0	39.0	32.0	39.0
12	34.61776842053881	35.0	33.0	39.0	30.0	39.0
13	33.921094861186624	35.0	33.0	38.0	25.0	39.0
14	34.41311635557523	35.0	33.0	39.0	25.0	41.0
15	35.04997512788506	35.0	33.0	39.0	30.0	41.0
16	35.38216285599319	35.0	34.0	39.0	31.0	41.0
17	35.28831550109203	35.0	34.0	39.0	31.0	41.0
18	35.43332169744397	36.0	34.0	39.0	31.0	41.0
19	35.26275615147295	36.0	34.0	38.0	30.0	41.0
20	34.9190694457067	35.0	34.0	38.0	30.0	41.0
21	34.724378751089056	35.0	34.0	38.0	29.0	41.0
22	34.532934116352685	35.0	33.0	38.0	29.0	41.0
23	34.57314730040409	35.0	34.0	38.0	29.0	41.0
24	34.699522371897366	35.0	34.0	38.0	30.0	41.0
25	34.651166741438225	35.0	34.0	38.0	30.0	41.0
26	34.42014895008846	35.0	34.0	38.0	29.0	40.0
27	34.37221786366998	35.0	34.0	38.0	29.0	40.0
28	34.532100121743305	35.0	34.0	38.0	29.0	40.0
29	34.58653810025855	35.0	34.0	38.0	29.0	40.0
30	34.569276306556766	35.0	34.0	38.0	29.0	40.0
31	34.20207525605112	35.0	34.0	38.0	29.0	40.0
32	34.02142373830617	35.0	34.0	38.0	28.0	41.0
33	33.7877873901428	35.0	33.0	38.0	27.0	41.0
34	33.631388191574054	35.0	33.0	38.0	25.0	41.0
35	33.39427199528313	35.0	33.0	38.0	24.0	41.0
36	33.06281003837917	35.0	33.0	38.0	23.0	40.0
37	33.111095339681796	35.0	32.0	38.0	23.0	40.0
38	33.236240774918116	35.0	32.0	38.0	23.0	40.0
39	33.276689674042025	35.0	33.0	37.0	23.0	40.0
40	32.874123976494005	35.0	32.0	37.0	23.0	40.0
41	33.03247312318296	35.0	32.0	37.0	23.0	40.0
42	33.01912246554112	35.0	32.0	37.0	23.0	40.0
43	33.00066571845408	35.0	32.0	37.0	23.0	40.0
44	33.00813152772591	35.0	33.0	37.0	23.0	40.0
45	33.11386322552634	35.0	33.0	37.0	23.0	40.0
46	33.16002998141576	35.0	33.0	37.0	23.0	40.0
47	33.10405278989983	35.0	33.0	37.0	23.0	40.0
48	33.1837948135619	35.0	33.0	37.0	23.0	40.0
49	33.29177094275431	35.0	33.0	37.0	24.0	40.0
50	33.01511177679388	35.0	32.0	37.0	24.0	40.0
51	32.69201706268833	35.0	32.0	36.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	9.0
12	1.0
13	6.0
14	21.0
15	74.0
16	284.0
17	821.0
18	2084.0
19	4189.0
20	7356.0
21	11863.0
22	17739.0
23	25868.0
24	35831.0
25	49151.0
26	62330.0
27	72427.0
28	80400.0
29	91594.0
30	109756.0
31	134989.0
32	174056.0
33	237138.0
34	464576.0
35	704511.0
36	130694.0
37	151522.0
38	216326.0
39	328115.0
40	190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.46439337569996	25.712692871288972	31.879146891274658	13.943766861736412
2	29.18637515498908	26.842069938010788	32.0836152654733	11.887939641526831
3	27.56064765766978	25.31631260699907	34.638972179519826	12.484067555811324
4	25.751454191794355	27.7397461534929	33.02731051350239	13.481489141210348
5	22.517436974317654	33.01584589757827	32.133905429443	12.33281169866108
6	19.117263110366356	41.01843683654958	29.87023788917474	9.994062163909325
7	86.79228074885458	5.336634200715559	6.6827471018125335	1.1883379486173256
8	90.17826032642363	3.0315720107940805	5.873094730162441	0.917072932619851
9	87.49977921783059	4.395122689052962	6.852468376767742	1.2526297163487028
10	72.79298917862289	13.31526826719556	9.354709115076162	4.537033439105388
11	71.01366151893636	11.799883683924714	12.77383010338386	4.41262469375506
12	61.568905392512164	19.838923751954525	13.408237631622303	5.183933223911015
13	16.395524753454556	63.67229310623331	13.648673428328006	6.283508711984121
14	7.013358364946663	63.19077923741999	23.604295409432908	6.191566988200437
15	6.6184553340811565	20.35984121667514	66.77284549519274	6.248857954050976
16	6.485022619333967	12.50747849421101	65.24660645762957	15.760892428825448
17	7.032080692912395	13.695174167426424	29.743966545158866	49.52877859450231
18	14.61215075873599	19.80456201795224	49.386386137898455	16.196901085413316
19	38.96238481994933	15.423537273971244	26.976755089791705	18.637322816287718
20	38.28613304927633	18.580160305517563	27.20890553381275	15.924801111393355
21	14.78142244090986	37.285018380316316	31.23651823789174	16.697040940882083
22	18.44470442325435	24.15356933314793	17.949156836909257	39.45256940668847
23	10.602457538370336	36.981864390613914	23.395138424800308	29.020539646215443
24	8.27324579333697	18.21502673953067	62.2215535421649	11.29017392496746
25	6.884100440311903	22.371319320382707	55.96537364853212	14.779206590773263
26	6.57776718737004	47.28845776509355	28.686395868370795	17.447379179165615
27	7.465070655111276	58.140953117428175	24.883483213650663	9.510493013809885
28	6.2111563879587495	37.11455848864891	46.90456975737084	9.769715366021511
29	6.195227957991335	25.894810061501083	45.65720669931781	22.252755281189778
30	12.346781188652663	48.318860192380754	28.027485533549417	11.30687308541717
31	38.76337578666694	29.84056476560641	22.978847623051134	8.417211824675515
32	42.40170536964716	19.860311522838188	29.783338027296065	7.954645080218592
33	41.94331341530266	26.552146821587776	17.7207315902193	13.78380817289026
34	18.969282857765865	26.707802265241114	22.03046376458808	32.29245111240494
35	18.16955364107531	22.394794486322585	38.152186514207614	21.28346535839449
36	45.63389210222841	23.447708666446797	20.7587263550325	10.159672876292298
37	14.83476341303864	49.15882796300109	26.831857759120393	9.174550864839885
38	14.758814346762565	49.17616939885271	18.273441687334554	17.79157456705018
39	20.41237934455153	42.71407601136699	24.87070193315262	12.002842710928862
40	31.72763412396365	21.18728461695819	22.092250658252002	24.992830600826156
41	11.6695338911067	21.48809430144361	25.667958389545813	41.17441341790388
42	14.810806540547327	17.934930436756908	25.14244865570153	42.11181436699424
43	16.55278588561268	17.4591328190206	29.20169342332468	36.78638787204204
44	9.776202347580822	30.770997026586027	37.679728728561244	21.773071897271905
45	8.684109367939987	60.3137386883259	19.224555216255734	11.777596727478372
46	18.6418187441011	47.17782582711423	20.27801533047157	13.902340098313095
47	17.363851263146977	29.46078732045593	23.03514306202871	30.140218354368386
48	18.147684163640214	25.837262185489777	42.93713825845098	13.077915392419031
49	23.254255315390942	24.88936003357816	40.77822583623455	11.078158814796355
50	18.86751432033293	50.12615894582054	20.398409854559947	10.607916879286586
51	15.235960742842886	44.32875637177341	21.790573901974	18.644708983409704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6421.0
1	6431.0
2	6441.0
3	12549.5
4	18658.0
5	15142.5
6	11627.0
7	12823.0
8	14019.0
9	15179.0
10	16339.0
11	16704.5
12	17070.0
13	16772.0
14	16474.0
15	15492.5
16	14511.0
17	12930.0
18	11349.0
19	10882.5
20	10416.0
21	11203.5
22	11991.0
23	12931.0
24	13871.0
25	14779.0
26	24729.5
27	33772.0
28	37936.5
29	42101.0
30	53018.5
31	63936.0
32	70688.0
33	77440.0
34	79294.0
35	81148.0
36	85414.0
37	89680.0
38	140745.0
39	191810.0
40	294116.5
41	396423.0
42	489245.5
43	582068.0
44	555750.5
45	529433.0
46	436872.0
47	344311.0
48	273301.0
49	202291.0
50	172025.0
51	141759.0
52	104025.0
53	66291.0
54	47573.0
55	28855.0
56	22251.5
57	15648.0
58	13490.5
59	11333.0
60	10380.5
61	9428.0
62	8371.0
63	7314.0
64	6029.0
65	4744.0
66	3921.5
67	3099.0
68	2518.5
69	1938.0
70	1796.5
71	1655.0
72	1371.0
73	1087.0
74	891.5
75	510.0
76	324.0
77	276.5
78	229.0
79	190.0
80	151.0
81	100.0
82	49.0
83	33.0
84	17.0
85	15.0
86	13.0
87	7.0
88	1.0
89	5.0
90	9.0
91	5.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3113929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.414823944675305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.59810844719493	10.006113266457724
2	8.422985461325982	2.0913976318184355
3	2.7649682151704496	1.0297978081189272
4	1.3267272482295642	0.6588434083749428
5	0.7955328022691244	0.4938199841192685
6	0.5791823600512529	0.43142682191387083
7	0.38767699876924067	0.33690591809841597
8	0.33595343150106255	0.3336642164556181
9	0.2809959912785799	0.3139664184794778
>10	2.6736900768599083	7.169057250420302
>50	0.47770395554806444	4.1388457380411
>100	0.8953981933594898	30.104002057819834
>500	0.39951332212075674	31.62808714187948
>1k	0.06130373473374421	10.955385447871729
>5k	2.597615878548483E-4	0.30868689013086986
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9572	0.30739300735501673	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4692	0.15067780928852262	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3995	0.12829451153189428	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3915	0.12572540992424683	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3622	0.11631607528623807	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3530	0.1133616084374435	No Hit
TTTGTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	3203	0.10286040561618456	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01448331031311247	0.0
2	0.0	0.0	0.0	0.06981533618781931	0.0
3	0.0	0.0	0.0	0.09871772927385307	0.0
4	0.0	0.0	0.0	0.14720952211819857	0.0
5	0.0	0.0	0.0	0.25652479552359736	0.0
6	0.0	0.0	0.0	0.35280187827018533	0.0
7	0.0	0.0	0.0	0.42537899868622564	0.0
8	0.0	0.0	0.0	0.5822547656031978	0.0
9	0.0	0.0	0.0	0.6318063128606979	0.0
10	0.0	0.0	0.0	0.7082049719181137	0.0
11	0.0	0.0	0.0	0.804385713354415	0.0
12	0.0	0.0	0.0	0.8839636356512945	0.0
13	0.0	0.0	0.0	0.9188391899751086	0.0
14	0.0	0.0	0.0	0.9312672190021032	0.0
15	0.0	0.0	0.0	0.953457834138158	0.0
16	0.0	0.0	0.0	1.0082439259212397	0.0
17	0.0	0.0	0.0	1.0712190290786976	0.0
18	0.0	0.0	0.0	1.1811123503458172	0.0
19	0.0	0.0	0.0	1.2213509042755952	0.0
20	0.0	0.0	0.0	1.2647366076747415	0.0
21	0.0	0.0	0.0	1.334295033701796	0.0
22	0.0	0.0	0.0	1.3992290768350852	0.0
23	0.0	0.0	0.0	1.4946069740189967	0.0
24	0.0	0.0	0.0	1.5569398017745426	0.0
25	0.0	0.0	0.0	1.6021881038392334	0.0
26	0.0	0.0	0.0	1.6628510155498086	0.0
27	0.0	0.0	0.0	1.7155175985065811	0.0
28	0.0	0.0	0.0	1.7686016604745967	0.0
29	0.0	0.0	0.0	1.8271771771289582	0.0
30	0.0	0.0	0.0	1.9312257922386797	0.0
31	0.0	0.0	0.0	2.0325126231201804	0.0
32	0.0	0.0	0.0	2.1078836415345372	0.0
33	0.0	0.0	0.0	2.1787266183654155	0.0
34	0.0	0.0	0.0	2.2599102291670747	0.0
35	0.0	0.0	0.0	2.4041973982065743	0.0
36	0.0	0.0	0.0	2.5032362651813833	0.0
37	0.0	0.0	0.0	2.597425952871758	0.0
38	0.0	0.0	0.0	2.683490856727947	0.0
39	0.0	0.0	0.0	2.772060634651593	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAG	75	0.0	45.0	1
TCGCCCG	40	6.8212103E-9	45.0	1
CTAGACC	20	7.0351467E-4	45.0	9
AACGTAC	20	7.0351467E-4	45.0	21
CTATGCG	135	0.0	45.0	1
AATCCCG	75	0.0	45.0	1
CTCACGA	50	2.1827873E-11	45.0	24
CGAACTA	45	3.8562575E-10	45.0	14
ATCTAGT	50	2.1827873E-11	45.0	19
CGCGGGT	20	7.0351467E-4	45.0	4
CCCTATG	50	2.1827873E-11	45.0	37
ACGTTAC	25	3.8923477E-5	45.0	25
GCGAAGT	95	0.0	45.0	2
GGCCGTA	45	3.8562575E-10	45.0	16
GGCAACG	150	0.0	45.0	1
CGACCTT	20	7.0351467E-4	45.0	44
CCTACCG	20	7.0351467E-4	45.0	41
GTTAGCG	75	0.0	45.0	1
TCGATTG	175	0.0	45.0	1
ATCGTTC	25	3.8923477E-5	45.0	22
>>END_MODULE