FastQCFastQC Report
Thu 26 May 2016
SRR1545080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545080_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2239214
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91370.4080449657781704No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG44510.19877510590769798No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG31150.13911131316613776No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG30830.13768224028610038No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24430.10910078268535298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA207.03472E-445.00000439
CCTTACG207.03472E-445.0000041
TTTACGC207.03472E-445.00000412
CGACGGA207.03472E-445.00000418
GACCGTA207.03472E-445.00000417
GTAGCAC453.8562575E-1045.00000414
TTGCGTG207.03472E-445.0000043
TCCGTTG207.03472E-445.00000443
ACGCTAG453.8562575E-1045.0000041
CGAGACG406.8193913E-945.00000433
GCTACGC453.8562575E-1045.00000410
GGTTCGC207.03472E-445.0000049
ACTCGAC351.2126111E-745.010
CAATGCG850.045.01
GTACGCG351.2126111E-745.01
ATCTACG502.1827873E-1144.9999961
CGACGGT1100.044.99999628
CGTTGTC650.044.9999963
CTATACG302.166429E-644.9999961
ACCGAGT302.166429E-644.99999621