##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545080_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2239214 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.912943559659773 31.0 31.0 33.0 30.0 33.0 2 31.232864299705163 31.0 31.0 33.0 30.0 34.0 3 31.392832931555446 31.0 31.0 34.0 30.0 34.0 4 35.233682443928984 37.0 35.0 37.0 33.0 37.0 5 35.110618279449845 37.0 35.0 37.0 32.0 37.0 6 35.17783338260657 37.0 35.0 37.0 32.0 37.0 7 35.5650210297006 36.0 35.0 37.0 35.0 37.0 8 35.628036891516395 37.0 35.0 37.0 35.0 37.0 9 37.47645825722776 39.0 37.0 39.0 35.0 39.0 10 37.01405180567824 39.0 37.0 39.0 33.0 39.0 11 36.5707614368256 39.0 35.0 39.0 32.0 39.0 12 35.0123708587031 35.0 34.0 39.0 30.0 39.0 13 34.5220162074728 35.0 33.0 39.0 27.0 39.0 14 35.09847026679898 35.0 33.0 40.0 27.0 41.0 15 35.605098485450696 35.0 34.0 40.0 30.0 41.0 16 35.86205650732802 35.0 34.0 40.0 31.0 41.0 17 35.71614504017928 35.0 34.0 40.0 31.0 41.0 18 35.82482290660919 36.0 35.0 39.0 31.0 41.0 19 35.69624028788673 36.0 35.0 39.0 31.0 41.0 20 35.398271893619814 35.0 34.0 39.0 30.0 41.0 21 35.23681970548594 35.0 34.0 39.0 30.0 41.0 22 35.073845108149555 35.0 34.0 39.0 30.0 41.0 23 35.02737299784656 35.0 34.0 39.0 29.0 41.0 24 35.143245799642195 35.0 34.0 39.0 30.0 41.0 25 35.075198708118116 35.0 34.0 39.0 30.0 41.0 26 34.823743956584764 35.0 34.0 39.0 29.0 41.0 27 34.80030716135215 35.0 34.0 39.0 29.0 41.0 28 34.90380553176249 35.0 34.0 39.0 29.0 41.0 29 34.91537700282331 36.0 34.0 39.0 29.0 41.0 30 34.87720200034477 36.0 34.0 39.0 29.0 41.0 31 34.600579489052855 35.0 34.0 39.0 29.0 41.0 32 34.44051037551569 35.0 34.0 39.0 29.0 41.0 33 34.1999866917588 35.0 33.0 39.0 27.0 41.0 34 34.02791068651768 35.0 34.0 39.0 25.0 41.0 35 33.826740990365366 35.0 33.0 39.0 24.0 41.0 36 33.57383349693241 35.0 33.0 39.0 23.0 41.0 37 33.59642311989832 35.0 33.0 39.0 23.0 41.0 38 33.666618733180485 35.0 33.0 39.0 24.0 41.0 39 33.68033247380554 35.0 33.0 39.0 24.0 41.0 40 33.340957139424816 35.0 32.0 39.0 23.0 41.0 41 33.4493782193216 35.0 33.0 39.0 23.0 41.0 42 33.42605530333412 35.0 33.0 39.0 23.0 41.0 43 33.390176642339675 35.0 33.0 39.0 23.0 41.0 44 33.38049110089523 35.0 33.0 38.0 23.0 41.0 45 33.456834407073195 35.0 33.0 38.0 23.0 41.0 46 33.48846425576117 35.0 33.0 38.0 23.0 41.0 47 33.413462491749335 35.0 33.0 38.0 23.0 41.0 48 33.45147583035833 35.0 33.0 38.0 23.0 41.0 49 33.54300839491 35.0 33.0 38.0 24.0 41.0 50 33.29959753735016 35.0 33.0 38.0 24.0 41.0 51 33.01158397544853 35.0 32.0 38.0 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 3.0 11 2.0 12 5.0 13 8.0 14 18.0 15 51.0 16 177.0 17 524.0 18 1241.0 19 2491.0 20 4692.0 21 7589.0 22 11554.0 23 16949.0 24 24072.0 25 33428.0 26 42858.0 27 49646.0 28 55182.0 29 62964.0 30 74588.0 31 91986.0 32 117892.0 33 158599.0 34 296258.0 35 443061.0 36 117865.0 37 140100.0 38 203258.0 39 282012.0 40 138.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.500517592333736 25.817541333700127 29.717034638047103 12.964906435919033 2 30.27080037906158 26.658059479799608 30.863731648694586 12.207408492444223 3 27.691949049979144 26.100944349222537 33.512652207426356 12.69445439337196 4 26.197987329482576 28.151395980911158 33.00318772569303 12.64742896391323 5 22.40576380819341 33.64953059421744 31.990778907241555 11.953926690347595 6 20.60182724831124 41.307262280425185 29.145986046889664 8.944924424373909 7 86.55885502680852 5.100807694128386 7.024205815076183 1.3161314639869168 8 88.70804666280222 3.2421644380572823 6.864015676929494 1.185773222211008 9 85.03684775104121 4.9453067013693195 7.974226670608527 2.0436188769809407 10 63.29841631929776 18.207504954863627 12.194948763271398 6.299129962567222 11 60.96014047786411 15.03898242865577 17.414548140552892 6.586328952927232 12 52.83126132651904 21.74906016128874 18.01377626256356 7.405902249628664 13 17.916777940831025 55.15600563411983 18.749882771365307 8.177333653683837 14 9.224174196838712 55.25921149117503 26.60455856385321 8.912055748133051 15 8.271697122293805 22.371421400545014 60.70161226215984 8.655269215001335 16 8.835689666106052 15.756823599709541 59.29808405985314 16.10940267433126 17 9.656959986852529 15.921434932078846 32.332639935262996 42.08896514580563 18 15.990610991178155 21.292292742006794 46.037582830403885 16.67951343641117 19 34.9408319169137 17.942769203836704 29.290858310103456 17.825540569146135 20 34.60843849672251 20.06279882137214 29.229988737119367 16.098773944785982 21 16.801341899434355 34.90121980302017 31.372436935460392 16.925001362085087 22 18.630465868827187 24.768467864170198 21.974407091059632 34.62665917594299 23 12.889299548859556 34.14975076075802 26.66377577131976 26.297173919062672 24 9.901778034613931 20.579408667505653 56.73321084987857 12.785602448001843 25 9.47345809735023 24.131324652311033 51.1738047368407 15.22141251349804 26 8.79505040607999 43.5525590676014 30.479578995129543 17.17281153118907 27 9.675046690490502 51.666566929288585 27.594012899169083 11.064373481051835 28 8.393748877954495 35.38764048456289 44.65031033210761 11.568300305375011 29 9.28227494111773 26.407927067265568 43.422066850242985 20.887731141373713 30 14.097669985986155 43.66871589763194 29.70475354298428 12.52886057339763 31 34.785330924154636 29.652458407280413 25.82513328337533 9.737077385189624 32 38.60528739102203 21.577705391266758 30.103956120317214 9.713051097393995 33 37.77003895116769 26.167262262561774 21.16188984170338 14.900808944567157 34 19.463079455558958 27.031270794126865 24.54754212862192 28.958107621692253 35 18.812404709866946 23.95751366327649 37.2159606004607 20.01412102639587 36 39.378371160594746 25.327994555232326 23.847966295316127 11.445667988856805 37 16.273344128788047 46.04236129284651 27.04471301090472 10.639581567460725 38 15.989985771793139 45.221180289155036 22.227308332298744 16.561525606753083 39 19.512561104030254 40.937445014188015 26.35415820015416 13.195835681627571 40 29.358248028102718 23.419333748359918 24.40494745031069 22.817470773226677 41 13.362590623316931 24.027627551453325 27.522559255167216 35.08722257006253 42 16.080106680290495 20.052572018574374 26.7800219184053 37.08729938272983 43 17.150392950383484 20.18167982158025 30.050901789645827 32.617025438390435 44 11.526142655413908 31.661332949865447 36.3211823434473 20.49134205127335 45 11.15909421788181 53.810086932289636 21.97217416468457 13.058644685143983 46 18.35684307082753 43.32207640716787 23.473236591053826 14.847843930950772 47 17.69527164442523 30.095381683036994 24.88029281703312 27.329053855504654 48 17.71737761553831 27.118399581281643 40.89823482704199 14.265987976138057 49 22.358425769042174 25.93159921293811 39.36711721166445 12.342857806355266 50 18.636673404149846 45.671963465751816 23.70023588634226 11.991127243756068 51 16.048979686622182 41.3192307657955 24.509671697300927 18.122117850281395 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2617.0 1 3929.0 2 5241.0 3 11773.5 4 18306.0 5 14490.0 6 10674.0 7 11185.0 8 11696.0 9 12482.5 10 13269.0 11 12862.0 12 12455.0 13 12029.5 14 11604.0 15 11129.0 16 10654.0 17 10306.5 18 9959.0 19 9519.0 20 9079.0 21 9796.0 22 10513.0 23 10014.5 24 9516.0 25 14192.0 26 22314.0 27 25760.0 28 35028.0 29 44296.0 30 51177.5 31 58059.0 32 65186.5 33 72314.0 34 80287.5 35 88261.0 36 93621.0 37 98981.0 38 117342.0 39 135703.0 40 200022.0 41 264341.0 42 313060.5 43 361780.0 44 344054.5 45 326329.0 46 271788.5 47 217248.0 48 180943.0 49 144638.0 50 119968.0 51 95298.0 52 74012.5 53 52727.0 54 39880.0 55 27033.0 56 22348.0 57 17663.0 58 15999.0 59 14335.0 60 13534.5 61 12734.0 62 10755.0 63 8776.0 64 7337.5 65 5899.0 66 4953.5 67 4008.0 68 3512.0 69 3016.0 70 2633.0 71 2250.0 72 1924.0 73 1598.0 74 1295.5 75 661.0 76 329.0 77 251.5 78 174.0 79 155.5 80 137.0 81 90.0 82 43.0 83 35.5 84 28.0 85 15.0 86 2.0 87 4.0 88 6.0 89 3.0 90 0.0 91 2.0 92 4.0 93 2.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2239214.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.741256019077644 #Duplication Level Percentage of deduplicated Percentage of total 1 80.33065694341335 11.841747801835533 2 7.32756866788133 2.1603513146122086 3 2.5125359824226794 1.1111380852211246 4 1.3948861779754085 0.8224949706803282 5 0.9472365001942142 0.6981727879989001 6 0.7055655362477538 0.6240553324839568 7 0.5349260374415924 0.551983716843806 8 0.4537652602865933 0.5351255899558461 9 0.3671933098415793 0.48716015299805004 >10 3.3091757468216665 9.9497022890184 >50 0.5270708796418222 5.56903669022308 >100 1.4830321467322072 53.65472796598656 >500 0.09574812998985255 9.35189398091549 >1k 0.010334718792555514 2.2329998732166656 >5k 3.0396231742810335E-4 0.4094094480100466 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9137 0.4080449657781704 No Hit AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 4451 0.19877510590769798 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 3115 0.13911131316613776 No Hit AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 3083 0.13768224028610038 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 2443 0.10910078268535298 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.016702289285436763 0.0 2 0.0 0.0 0.0 0.0555105496839516 0.0 3 0.0 0.0 0.0 0.07739322815952383 0.0 4 0.0 0.0 0.0 0.11441514745799196 0.0 5 0.0 0.0 0.0 0.2074835187704257 0.0 6 0.0 0.0 0.0 0.2922454039676422 0.0 7 0.0 0.0 0.0 0.3514626114341908 0.0 8 0.0 0.0 0.0 0.49651350875798383 0.0 9 0.0 0.0 0.0 0.5485406932968443 0.0 10 0.0 0.0 0.0 0.6404479428942477 0.0 11 0.0 0.0 0.0 0.7288271688190588 0.0 12 0.0 0.0 0.0 0.8102843229811889 0.0 13 0.0 0.0 0.0 0.8435995844970602 0.0 14 0.0 0.0 0.0 0.8545409237348462 0.0 15 0.0 0.0 0.0 0.8779866506729593 0.0 16 0.0 0.0 0.0 0.9280042014742673 0.0 17 0.0 0.0 0.0 0.9867748236658042 0.0 18 0.0 0.0 0.0 1.0843090477283548 0.0 19 0.0 0.0 0.0 1.1260647709419467 0.0 20 0.0 0.0 0.0 1.173536785675688 0.0 21 0.0 0.0 0.0 1.2381576749698777 0.0 22 0.0 0.0 0.0 1.3075570267066927 0.0 23 0.0 0.0 0.0 1.3917383510463939 0.0 24 0.0 0.0 0.0 1.451402143787954 0.0 25 0.0 0.0 0.0 1.5003032314017328 0.0 26 0.0 0.0 0.0 1.558359317153251 0.0 27 0.0 0.0 0.0 1.6080195997345497 0.0 28 0.0 0.0 0.0 1.6662989781235737 0.0 29 0.0 0.0 0.0 1.7308752088902624 0.0 30 0.0 0.0 0.0 1.825729921302743 0.0 31 0.0 0.0 0.0 1.9211651945727384 0.0 32 0.0 0.0 0.0 2.0041853971974093 0.0 33 0.0 0.0 0.0 2.0794350160368773 0.0 34 0.0 0.0 0.0 2.1691986563142245 0.0 35 0.0 0.0 0.0 2.2937959480424825 0.0 36 0.0 0.0 0.0 2.3967338539326746 0.0 37 0.0 0.0 0.0 2.49560783382026 0.0 38 0.0 0.0 0.0 2.5885868880776917 0.0 39 0.0 0.0 0.0 2.6793330159600646 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCATA 20 7.03472E-4 45.000004 39 CCTTACG 20 7.03472E-4 45.000004 1 TTTACGC 20 7.03472E-4 45.000004 12 CGACGGA 20 7.03472E-4 45.000004 18 GACCGTA 20 7.03472E-4 45.000004 17 GTAGCAC 45 3.8562575E-10 45.000004 14 TTGCGTG 20 7.03472E-4 45.000004 3 TCCGTTG 20 7.03472E-4 45.000004 43 ACGCTAG 45 3.8562575E-10 45.000004 1 CGAGACG 40 6.8193913E-9 45.000004 33 GCTACGC 45 3.8562575E-10 45.000004 10 GGTTCGC 20 7.03472E-4 45.000004 9 ACTCGAC 35 1.2126111E-7 45.0 10 CAATGCG 85 0.0 45.0 1 GTACGCG 35 1.2126111E-7 45.0 1 ATCTACG 50 2.1827873E-11 44.999996 1 CGACGGT 110 0.0 44.999996 28 CGTTGTC 65 0.0 44.999996 3 CTATACG 30 2.166429E-6 44.999996 1 ACCGAGT 30 2.166429E-6 44.999996 21 >>END_MODULE