FastQCFastQC Report
Thu 26 May 2016
SRR1545079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545079_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3318267
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157830.47563984453330604No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAAG33740.10167958154060538No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33600.10125767456325849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGT302.1667456E-645.00000414
ACGGGTA2500.045.0000045
CGTTACC600.045.00000442
TACGAGT453.8562575E-1045.00000429
CTCGACG453.8562575E-1045.0000041
TTACGCG600.045.0000041
GCTCGAT302.1667456E-645.00000438
TGCAACG453.8562575E-1045.0000041
TCGTTAG850.045.01
TCCGATT950.045.043
GCAACGA207.0352136E-445.020
GCACGTT253.8924038E-545.036
TACGCCT207.0352136E-445.04
GTATCGA406.8212103E-945.045
TAGTGCA351.2128476E-745.017
TAGTCCG700.045.01
TACCGCG351.2128476E-745.01
AAGTCGC406.8212103E-945.042
CGGCGTA650.044.99999621
TTACGTG2600.044.1346131