##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545079_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3318267
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.063855621021453	31.0	31.0	33.0	30.0	33.0
2	31.39438056069629	31.0	31.0	33.0	30.0	34.0
3	31.51865717858147	31.0	31.0	34.0	30.0	34.0
4	35.384019128056906	37.0	35.0	37.0	33.0	37.0
5	35.292537942245154	37.0	35.0	37.0	33.0	37.0
6	35.35313614003936	37.0	35.0	37.0	33.0	37.0
7	35.69772263654492	37.0	35.0	37.0	35.0	37.0
8	35.76458374205572	37.0	35.0	37.0	35.0	37.0
9	37.62362462092411	39.0	37.0	39.0	35.0	39.0
10	37.18973217043716	39.0	37.0	39.0	34.0	39.0
11	36.77229318798035	39.0	35.0	39.0	33.0	39.0
12	35.25028878025789	35.0	35.0	39.0	31.0	39.0
13	34.7294533562248	35.0	34.0	39.0	30.0	39.0
14	35.312715944798896	35.0	33.0	40.0	29.0	41.0
15	35.78424581264859	35.0	34.0	40.0	31.0	41.0
16	36.00745419220334	35.0	35.0	40.0	32.0	41.0
17	35.885624032062516	35.0	34.0	40.0	32.0	41.0
18	35.98891921596424	36.0	35.0	39.0	31.0	41.0
19	35.87959257045922	36.0	35.0	40.0	31.0	41.0
20	35.594271949785835	35.0	34.0	40.0	31.0	41.0
21	35.42092483817607	35.0	34.0	40.0	31.0	41.0
22	35.25950805043717	35.0	34.0	39.0	30.0	41.0
23	35.22913677531073	35.0	34.0	39.0	30.0	41.0
24	35.31289917297192	35.0	34.0	39.0	31.0	41.0
25	35.19202402941053	35.0	34.0	39.0	31.0	41.0
26	34.96820870653266	35.0	34.0	39.0	30.0	41.0
27	34.948802191023205	35.0	34.0	39.0	30.0	41.0
28	35.07275484462221	35.0	34.0	39.0	30.0	41.0
29	35.09446798584924	36.0	34.0	39.0	30.0	41.0
30	35.04646732767436	36.0	34.0	39.0	30.0	41.0
31	34.7694694248534	35.0	34.0	39.0	29.0	41.0
32	34.60965256864502	35.0	34.0	39.0	29.0	41.0
33	34.340922234407294	35.0	33.0	39.0	27.0	41.0
34	34.16072968209008	35.0	34.0	39.0	27.0	41.0
35	33.93082654289121	35.0	33.0	39.0	25.0	41.0
36	33.67283283714059	35.0	33.0	39.0	23.0	41.0
37	33.68690042121384	35.0	33.0	39.0	23.0	41.0
38	33.7289901626361	35.0	33.0	39.0	24.0	41.0
39	33.71633626829909	35.0	33.0	39.0	24.0	41.0
40	33.39568365053204	35.0	33.0	39.0	23.0	41.0
41	33.47912509752832	35.0	33.0	39.0	23.0	41.0
42	33.47000497548871	35.0	33.0	39.0	23.0	41.0
43	33.42552362422915	35.0	33.0	39.0	23.0	41.0
44	33.39754636983702	35.0	33.0	38.0	23.0	41.0
45	33.434906232681094	35.0	33.0	38.0	23.0	41.0
46	33.49270146133509	35.0	33.0	38.0	23.0	41.0
47	33.43988804999718	35.0	33.0	38.0	23.0	41.0
48	33.473941970311614	35.0	33.0	38.0	23.0	41.0
49	33.54262239898115	35.0	33.0	38.0	24.0	41.0
50	33.294733968062246	35.0	33.0	38.0	24.0	40.0
51	32.99125416972173	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	6.0
11	1.0
12	8.0
13	10.0
14	28.0
15	77.0
16	221.0
17	576.0
18	1482.0
19	3144.0
20	5736.0
21	9553.0
22	15112.0
23	22976.0
24	33271.0
25	47117.0
26	60913.0
27	69301.0
28	75401.0
29	85812.0
30	103045.0
31	129498.0
32	170084.0
33	233584.0
34	450423.0
35	683507.0
36	179522.0
37	214514.0
38	306679.0
39	416461.0
40	201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.747766831300797	24.114394652389333	30.88401264877118	14.253825867538689
2	32.06697954082659	25.192728614062705	30.54003791738278	12.200253927727937
3	26.764874556507962	26.104499728322043	34.379120185325654	12.751505529844342
4	25.633741950240896	27.893385312272944	33.90896513149786	12.563907605988305
5	22.59908560703524	32.160190846607584	32.56208737874318	12.678636167613998
6	20.59231520549733	39.863699937346816	30.2433469036699	9.300637953485962
7	85.68496748453335	4.954001591794753	8.139037636211913	1.2219932874599904
8	87.65304298900601	3.065425416339312	8.05137139356176	1.2301602010929198
9	84.48702289478213	4.379394424860929	9.267096348786882	1.8664863315700635
10	63.280441266480366	16.604781953953676	13.505393025937936	6.609383753628023
11	60.19482458765374	14.282545678210944	18.57083833217761	6.951791401957709
12	51.831242030855265	20.789406036343667	19.34823207415196	8.031119858649108
13	17.75071144064055	54.17565855912137	19.721077297275958	8.352552702962118
14	10.204935287003728	52.85861565690766	27.98575280409925	8.950696251989367
15	7.809678967967315	21.603957728537214	61.816333646448584	8.770029657046887
16	9.050537524557246	15.661940404434002	59.10356219074595	16.183959880262798
17	10.071582545949436	15.613300557188436	32.041544577335095	42.273572319527034
18	16.36064849513315	20.797241451637255	47.04214579477782	15.799964258451777
19	35.03614989390546	17.651201666412017	29.23526045372479	18.07738798595773
20	34.21623395585708	19.969701051783957	30.038812428294648	15.775252564064315
21	17.08997497790262	33.49203062924111	32.311233544497775	17.106760848358498
22	18.991298771316472	23.080330787124726	23.0444084216249	34.883962019933904
23	11.914894129978087	34.13218405872704	27.387398301583328	26.565523509711547
24	10.354802672599885	20.253463630262424	56.87987133042639	12.511862366711298
25	9.786403565475593	23.662140508886115	50.96672449805878	15.584731427579518
26	8.6710321984337	43.5147623744563	30.70195978804599	17.112245639064007
27	8.98101328193301	51.700872774855064	28.515246060669618	10.802867882542303
28	8.205337304080714	33.33640722702543	46.88585939588346	11.572396073010399
29	9.021305398269638	25.35567511595661	44.99083407091714	20.63218541485661
30	14.547684077260811	41.71418393999036	31.17868453623533	12.559447446513497
31	34.31779299254701	28.702331668910308	27.241870530611312	9.73800480793137
32	38.758062566996564	20.583425022760373	30.701899515620656	9.956612894622403
33	37.640280302941264	25.67683673435561	21.401593060474035	15.281289902229087
34	19.205687788234037	25.762363305906366	25.716495990226225	29.315452915633372
35	18.765247040096533	23.6606638344654	37.08827529550817	20.4858138299299
36	39.56095154488774	25.05292672349754	23.638543854367356	11.74757787724737
37	16.415436129762913	45.16538301468809	27.715099478131204	10.70408137741779
38	16.61083933269987	44.82282468529507	21.9049280844489	16.661407897556163
39	19.434270961318063	40.82016305499226	26.48065993483948	13.264906048850197
40	29.032564287322266	23.437294226172877	24.89245139104237	22.63769009546248
41	13.468476165420082	23.05959707280939	27.948775671156056	35.52315109061447
42	16.49568886409683	19.379151828348952	26.883219463653766	37.24193984390045
43	16.31155660469757	19.676234612826516	30.752016037286932	33.26019274518898
44	11.230561012721399	30.699669435883248	36.98828334187695	21.081486209518403
45	10.988024773172262	52.69325825800033	22.546980095332895	13.771736873494508
46	18.34795090328777	42.40252517353185	24.323359151026725	14.926164772153658
47	16.92003687466982	29.534513045514423	25.872571435631915	27.672878644183847
48	18.10900087304608	26.59659394497188	41.36894348766992	13.925461694312121
49	22.546226690016205	26.056040698352483	39.56212685718178	11.835605754449537
50	18.392251135909195	44.98242606758287	24.255401991461206	12.36992080504673
51	15.490555762993152	40.886251769372386	25.3013997969422	18.32179267069226
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5037.0
1	7639.0
2	10241.0
3	20727.5
4	31214.0
5	24794.5
6	18375.0
7	19374.5
8	20374.0
9	21847.0
10	23320.0
11	23241.0
12	23162.0
13	23054.0
14	22946.0
15	21213.5
16	19481.0
17	18555.0
18	17629.0
19	16430.5
20	15232.0
21	15965.5
22	16699.0
23	17288.0
24	17877.0
25	22398.0
26	30552.0
27	34185.0
28	40350.5
29	46516.0
30	62869.5
31	79223.0
32	90395.0
33	101567.0
34	104955.5
35	108344.0
36	117546.0
37	126748.0
38	165888.5
39	205029.0
40	292971.5
41	380914.0
42	458516.0
43	536118.0
44	503066.0
45	470014.0
46	401148.0
47	332282.0
48	282984.5
49	233687.0
50	189452.0
51	145217.0
52	114171.0
53	83125.0
54	63416.5
55	43708.0
56	35984.0
57	28260.0
58	25618.5
59	22977.0
60	20686.0
61	18395.0
62	17208.0
63	16021.0
64	13796.5
65	11572.0
66	9720.5
67	7869.0
68	6894.5
69	5920.0
70	5355.0
71	4790.0
72	4018.0
73	3246.0
74	2608.5
75	1504.5
76	1038.0
77	766.0
78	494.0
79	374.5
80	255.0
81	187.5
82	120.0
83	96.5
84	73.0
85	50.5
86	28.0
87	26.5
88	25.0
89	19.0
90	13.0
91	10.5
92	8.0
93	7.0
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3318267.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.247473514277011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.1599875522117	11.420772995549111
2	7.773462547893205	2.2150440353066547
3	2.6397646551261023	1.128299310235012
4	1.3695305889186775	0.7804940317044423
5	0.9119357577070346	0.6496390277326558
6	0.6636135370562585	0.5672889775754838
7	0.5146794559826712	0.5133017342218927
8	0.40145239037519315	0.45757458392910083
9	0.3505666907655526	0.4495220677502955
>10	3.4387554634676873	10.267882257431465
>50	0.48580686556965413	4.876512359248002
>100	1.0019296193759937	37.36124396061823
>500	0.25979104944461484	23.231877426577555
>1k	0.02851105702340572	5.6020979192907845
>5k	0.0	0.0
>10k+	2.1276908226422182E-4	0.4784493128292945
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15783	0.47563984453330604	No Hit
GGCTCTGGGCTCCTCTACAAAAACAAAAGTTAAAAAAAAACACACAAAAAG	3374	0.10167958154060538	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3360	0.10125767456325849	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01383252161444513	0.0
2	0.0	0.0	0.0	0.05909711304123508	0.0
3	0.0	0.0	0.0	0.08661147520678716	0.0
4	0.0	0.0	0.0	0.12425160482866508	0.0
5	0.0	0.0	0.0	0.21526296708492718	0.0
6	0.0	0.0	0.0	0.30075940242301175	0.0
7	0.0	0.0	0.0	0.36308109021968393	0.0
8	0.0	0.0	0.0	0.48257117344686246	0.0
9	0.0	0.0	0.0	0.5364547216965965	0.0
10	0.0	0.0	0.0	0.6286413962468963	0.0
11	0.0	0.0	0.0	0.7419234196645418	0.0
12	0.0	0.0	0.0	0.8335676423868242	0.0
13	0.0	0.0	0.0	0.8744323467641393	0.0
14	0.0	0.0	0.0	0.8908867188806687	0.0
15	0.0	0.0	0.0	0.9177682205802005	0.0
16	0.0	0.0	0.0	0.9810844033949047	0.0
17	0.0	0.0	0.0	1.0534715862225674	0.0
18	0.0	0.0	0.0	1.1688028721016122	0.0
19	0.0	0.0	0.0	1.2195221180212443	0.0
20	0.0	0.0	0.0	1.2781370516597972	0.0
21	0.0	0.0	0.0	1.3604691846677799	0.0
22	0.0	0.0	0.0	1.4355384904228623	0.0
23	0.0	0.0	0.0	1.53438526797271	0.0
24	0.0	0.0	0.0	1.6038191019589443	0.0
25	0.0	0.0	0.0	1.6659599724796106	0.0
26	0.0	0.0	0.0	1.7352732616151745	0.0
27	0.0	0.0	0.0	1.8067864942754757	0.0
28	0.0	0.0	0.0	1.8745929727776578	0.0
29	0.0	0.0	0.0	1.9562018366816172	0.0
30	0.0	0.0	0.0	2.0735522488093934	0.0
31	0.0	0.0	0.0	2.1770098668973894	0.0
32	0.0	0.0	0.0	2.2669363254976167	0.0
33	0.0	0.0	0.0	2.3581285050298844	0.0
34	0.0	0.0	0.0	2.4650819237873263	0.0
35	0.0	0.0	0.0	2.6159438044015144	0.0
36	0.0	0.0	0.0	2.72820119658846	0.0
37	0.0	0.0	0.0	2.834732708368555	0.0
38	0.0	0.0	0.0	2.9558200108671184	0.0
39	0.0	0.0	0.0	3.129103233706028	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGT	30	2.1667456E-6	45.000004	14
ACGGGTA	250	0.0	45.000004	5
CGTTACC	60	0.0	45.000004	42
TACGAGT	45	3.8562575E-10	45.000004	29
CTCGACG	45	3.8562575E-10	45.000004	1
TTACGCG	60	0.0	45.000004	1
GCTCGAT	30	2.1667456E-6	45.000004	38
TGCAACG	45	3.8562575E-10	45.000004	1
TCGTTAG	85	0.0	45.0	1
TCCGATT	95	0.0	45.0	43
GCAACGA	20	7.0352136E-4	45.0	20
GCACGTT	25	3.8924038E-5	45.0	36
TACGCCT	20	7.0352136E-4	45.0	4
GTATCGA	40	6.8212103E-9	45.0	45
TAGTGCA	35	1.2128476E-7	45.0	17
TAGTCCG	70	0.0	45.0	1
TACCGCG	35	1.2128476E-7	45.0	1
AAGTCGC	40	6.8212103E-9	45.0	42
CGGCGTA	65	0.0	44.999996	21
TTACGTG	260	0.0	44.134613	1
>>END_MODULE