##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545078_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1585489
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.946785502769178	31.0	31.0	33.0	30.0	33.0
2	31.25910555040117	31.0	31.0	33.0	30.0	34.0
3	31.42328392060746	31.0	31.0	34.0	30.0	34.0
4	35.28710763682372	37.0	35.0	37.0	33.0	37.0
5	35.16237892536624	37.0	35.0	37.0	32.0	37.0
6	35.25328274116061	37.0	35.0	37.0	32.0	37.0
7	35.57844614500637	36.0	35.0	37.0	35.0	37.0
8	35.630549943897435	37.0	35.0	37.0	35.0	37.0
9	37.468915899132696	39.0	37.0	39.0	35.0	39.0
10	37.07105252701217	39.0	37.0	39.0	33.0	39.0
11	36.651451381876505	39.0	35.0	39.0	32.0	39.0
12	35.08787446648952	35.0	35.0	39.0	31.0	39.0
13	34.56731456351952	35.0	33.0	39.0	29.0	39.0
14	35.1715344603463	35.0	33.0	40.0	27.0	41.0
15	35.63480541334566	35.0	34.0	40.0	30.0	41.0
16	35.86487071181194	35.0	34.0	40.0	32.0	41.0
17	35.73932963268746	35.0	34.0	40.0	31.0	41.0
18	35.873300918517884	36.0	35.0	39.0	31.0	41.0
19	35.7547343437892	36.0	35.0	39.0	31.0	41.0
20	35.465789418911136	35.0	34.0	39.0	31.0	41.0
21	35.31356698154323	35.0	34.0	39.0	30.0	41.0
22	35.14181681487541	35.0	34.0	39.0	30.0	41.0
23	35.1013100690071	35.0	34.0	39.0	30.0	41.0
24	35.20795981555217	35.0	34.0	39.0	30.0	41.0
25	35.12911221711409	35.0	34.0	39.0	30.0	41.0
26	34.92477462789083	35.0	34.0	39.0	30.0	41.0
27	34.89506770466399	35.0	34.0	39.0	29.0	41.0
28	35.0258885429038	35.0	34.0	39.0	30.0	41.0
29	35.048874511270654	36.0	34.0	39.0	30.0	41.0
30	35.006341891996726	36.0	34.0	39.0	29.0	41.0
31	34.70890494982936	35.0	34.0	39.0	29.0	41.0
32	34.53960197768638	35.0	34.0	39.0	29.0	41.0
33	34.32234660726123	35.0	33.0	39.0	27.0	41.0
34	34.15908530428152	35.0	34.0	39.0	27.0	41.0
35	33.95353736292084	35.0	33.0	39.0	25.0	41.0
36	33.681380949347485	35.0	33.0	39.0	23.0	41.0
37	33.73572632796569	35.0	33.0	39.0	24.0	41.0
38	33.80469621675079	35.0	33.0	39.0	24.0	41.0
39	33.82415772042569	35.0	33.0	39.0	24.0	41.0
40	33.4965572135789	35.0	33.0	39.0	23.0	41.0
41	33.62011089323231	35.0	33.0	39.0	23.0	41.0
42	33.59207348647641	35.0	33.0	39.0	23.0	41.0
43	33.58326926266912	35.0	33.0	39.0	23.0	41.0
44	33.57285544081353	35.0	33.0	39.0	23.0	41.0
45	33.620466619446745	35.0	33.0	39.0	23.0	41.0
46	33.66407272456637	35.0	33.0	39.0	24.0	41.0
47	33.61063242949021	35.0	33.0	38.0	24.0	41.0
48	33.64773202463088	35.0	33.0	38.0	23.0	41.0
49	33.71024523033588	35.0	33.0	38.0	24.0	41.0
50	33.46914106625779	35.0	33.0	38.0	24.0	41.0
51	33.178112872432415	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	0.0
12	5.0
13	10.0
14	18.0
15	40.0
16	98.0
17	272.0
18	748.0
19	1568.0
20	2710.0
21	4655.0
22	7263.0
23	10861.0
24	15821.0
25	21990.0
26	28359.0
27	33059.0
28	37120.0
29	42143.0
30	51593.0
31	64394.0
32	84251.0
33	114937.0
34	217496.0
35	313925.0
36	83766.0
37	101502.0
38	146334.0
39	200470.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.45030397561888	25.17135092075694	29.750884427454245	12.627460676169939
2	30.326290500911707	27.377925674665672	30.601284524837446	11.694499299585177
3	28.945202394970888	26.007181380633988	32.88928526151869	12.158330962876438
4	28.64649329008274	27.121727113843107	31.517342599034116	12.71443699704003
5	22.18243078318424	33.685128058283595	31.01655072977485	13.115890428757309
6	20.411620641959676	41.59303533483991	28.5798261608879	9.41551786231251
7	85.6083517451083	5.658632762510494	7.251705940564709	1.4813095518165058
8	88.05649235030958	4.2842302910963115	6.461476553921219	1.19780080467288
9	83.05633151664881	7.004778967246067	8.04256604744656	1.8963234686585653
10	63.42327193692293	18.50009681555659	12.009102554480037	6.067528693040444
11	61.97078629999956	15.458133105937662	16.25485891103628	6.316221683026499
12	53.9224806983839	21.730141300255003	17.436576349630933	6.91080165173016
13	17.524751038953912	56.6241077673828	17.789779683113537	8.061361510549741
14	9.472850331979597	56.3277323273766	25.096421356439556	9.102995984204243
15	9.494294820083898	22.515829501182285	59.49590315669172	8.493972522042096
16	9.54740146415396	17.013173853618664	58.03887633405215	15.400548348175233
17	9.582532581430714	17.746764562857265	30.805700954090504	41.86500190162152
18	16.031394730584697	22.71646160900517	45.35471390845348	15.897429751956652
19	36.82466418877709	17.897254411730387	27.815140943898065	17.462940455594456
20	35.44395451497929	21.532978153743105	28.108615070807808	14.9144522604698
21	16.77961814935329	37.428137312841656	29.557253314277173	16.234991223527885
22	18.48237357685862	25.879334388318053	20.45192366519099	35.18636836963233
23	13.315008808008127	35.71459656926034	25.312506110102312	25.657888512629228
24	10.04201227507728	21.38210987272696	56.4739963506527	12.101881501543058
25	8.665780714971847	26.31339605635864	50.45578998025215	14.565033248417365
26	8.571551111360597	46.24447094871046	28.667811634139373	16.51616630578957
27	9.99281609648506	52.95041466702071	26.622764333275097	10.434004903219133
28	7.941966169427856	35.765243404400785	45.400567269782385	10.892223156388974
29	9.110249266945402	27.975470028489635	42.51489603522951	20.399384669335454
30	14.557086173414008	45.78177458184825	27.859291360583395	11.801847884154352
31	36.38278158978082	30.076525286520432	24.29181154836142	9.248881575337325
32	38.850852954514345	22.650172911953348	28.653241996633216	9.84573213689909
33	37.874813385649475	27.44276371516926	20.346593385384573	14.335829513796691
34	19.10672354081296	27.210784811499796	23.81095043863439	29.871541209052854
35	19.768285998830645	23.76326798861424	36.46723502969746	20.001210982857653
36	39.40765278094014	26.94777447210293	22.47281438092601	11.171758366030922
37	15.527890764300478	48.456280680597594	26.496872573698084	9.518955981403844
38	15.363840430302576	47.82966012378515	20.5392784182041	16.26722102770817
39	20.633066517648498	42.676486560297796	24.626030202669334	12.064416719384367
40	30.055711518654498	23.59209051592285	23.929904275589422	22.422293689833232
41	12.711157251800548	24.22545977928576	26.135974453307465	36.927408515606224
42	14.672949481201067	21.422349823934443	25.896931482968345	38.00776921189614
43	16.737486037430724	20.851043432026334	28.301867751841858	34.10960277870108
44	11.127923309464776	32.79221741683481	36.27366698854423	19.806192285156186
45	10.850280260537916	56.09310439870602	19.86264174648957	13.1939735942665
46	16.81531691484457	46.73517129415593	21.841652638397367	14.607859152602131
47	17.21115693644043	30.292357752087838	24.320446247183046	28.176039064288684
48	17.870574945647682	27.703314245636516	40.936644782776796	13.489466025939
49	21.632316591285086	28.266610490517436	38.925845590855566	11.175227327341911
50	17.856699100403723	47.681125507650954	22.532165155355855	11.930010236589467
51	16.96845578871881	42.23636997796894	23.55677018257459	17.23840405073766
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1805.0
1	2696.0
2	3587.0
3	7744.5
4	11902.0
5	9707.0
6	7512.0
7	7780.0
8	8048.0
9	8382.5
10	8717.0
11	8700.0
12	8683.0
13	8236.0
14	7789.0
15	7532.5
16	7276.0
17	6361.5
18	5447.0
19	5701.5
20	5956.0
21	7243.5
22	8531.0
23	8914.0
24	9297.0
25	11313.5
26	16502.0
27	19674.0
28	28225.0
29	36776.0
30	41657.5
31	46539.0
32	54247.0
33	61955.0
34	61992.0
35	62029.0
36	61653.0
37	61277.0
38	74694.5
39	88112.0
40	137163.0
41	186214.0
42	224019.5
43	261825.0
44	248543.5
45	235262.0
46	195378.5
47	155495.0
48	126158.0
49	96821.0
50	80988.5
51	65156.0
52	49873.5
53	34591.0
54	25983.5
55	17376.0
56	14560.0
57	11744.0
58	10580.0
59	9416.0
60	8583.0
61	7750.0
62	6901.5
63	6053.0
64	5163.0
65	4273.0
66	3588.5
67	2904.0
68	2521.5
69	2139.0
70	1867.0
71	1595.0
72	1352.0
73	1109.0
74	940.0
75	580.5
76	390.0
77	279.0
78	168.0
79	143.0
80	118.0
81	78.0
82	38.0
83	32.0
84	26.0
85	15.5
86	5.0
87	4.0
88	3.0
89	3.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1585489.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.616519467450805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.87856386584095	11.675465837770767
2	6.686308224622373	1.954611086611387
3	2.5486528235813473	1.1175730083495066
4	1.5124265411105948	0.8842564792492917
5	1.0287098163170285	0.7518078528277796
6	0.8331470356338164	0.7306625919354365
7	0.648311905087072	0.6633244507179715
8	0.5275479103391585	0.6168731441188241
9	0.4382604451914014	0.5765258096058399
>10	3.374480016825471	9.77857480763135
>50	0.6987874790091233	7.426840111874768
>100	1.7495632054130374	54.758195482409086
>500	0.06615995314587116	6.394205638689515
>1k	0.007351105905096795	1.3081427155491108
>5k	0.001729671977669834	1.3629409826593721
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5689	0.35881674360402377	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5551	0.350112804314631	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5241	0.33056047692541546	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5083	0.3205950971592991	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1661	0.10476263159189374	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01608336607822571	0.0
2	0.0	0.0	0.0	0.050079186925926326	0.0
3	0.0	0.0	0.0	0.06912693812445246	0.0
4	0.0	0.0	0.0	0.09832928516060345	0.0
5	0.0	0.0	0.0	0.18101670840983444	0.0
6	0.0	0.0	0.0	0.24598089296109907	0.0
7	0.0	0.0	0.0	0.2915188941708205	0.0
8	0.0	0.0	0.0	0.3841716971861678	0.0
9	0.0	0.0	0.0	0.4182305900577046	0.0
10	0.0	0.0	0.0	0.4842039269903481	0.0
11	0.0	0.0	0.0	0.5492311835654489	0.0
12	0.0	0.0	0.0	0.6128708556161537	0.0
13	6.307202383617925E-5	0.0	0.0	0.6408748341994174	0.0
14	6.307202383617925E-5	0.0	0.0	0.6503356377748443	0.0
15	6.307202383617925E-5	0.0	0.0	0.6679958044489744	0.0
16	6.307202383617925E-5	0.0	0.0	0.7070373872035693	0.0
17	6.307202383617925E-5	0.0	0.0	0.7542783330568676	0.0
18	6.307202383617925E-5	0.0	0.0	0.8307216259463169	0.0
19	6.307202383617925E-5	0.0	0.0	0.8653481670323793	0.0
20	6.307202383617925E-5	0.0	0.0	0.8976410432365031	0.0
21	6.307202383617925E-5	0.0	0.0	0.9545320087367367	0.0
22	6.307202383617925E-5	0.0	0.0	1.0090893093550317	0.0
23	6.307202383617925E-5	0.0	0.0	1.0737381337871155	0.0
24	6.307202383617925E-5	0.0	0.0	1.1217359439264478	0.0
25	6.307202383617925E-5	0.0	0.0	1.1625435433484559	0.0
26	6.307202383617925E-5	0.0	0.0	1.2112982177738225	0.0
27	6.307202383617925E-5	0.0	0.0	1.252231961243503	0.0
28	6.307202383617925E-5	0.0	0.0	1.2960039457858112	0.0
29	6.307202383617925E-5	0.0	0.0	1.3482906535460037	0.0
30	6.307202383617925E-5	0.0	0.0	1.4258692428645043	0.0
31	6.307202383617925E-5	0.0	0.0	1.5022494637301174	0.0
32	6.307202383617925E-5	0.0	0.0	1.5652584155424605	0.0
33	6.307202383617925E-5	0.0	0.0	1.6275735750926055	0.0
34	6.307202383617925E-5	0.0	0.0	1.7003586905995562	0.0
35	6.307202383617925E-5	0.0	0.0	1.805436682310631	0.0
36	6.307202383617925E-5	0.0	0.0	1.8891332579412408	0.0
37	6.307202383617925E-5	0.0	0.0	1.97333440976254	0.0
38	6.307202383617925E-5	0.0	0.0	2.048894694318283	0.0
39	6.307202383617925E-5	0.0	0.0	2.1275455080419983	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCACGG	165	0.0	45.000004	2
GCATAAC	20	7.034098E-4	45.0	13
CTTCGAC	25	3.8914783E-5	45.0	23
GATACCC	25	3.8914783E-5	45.0	8
TCGTTAA	25	3.8914783E-5	45.0	30
TCGCACG	40	6.8175723E-9	45.0	1
AAATCGG	45	3.8562575E-10	45.0	2
AGTTTCG	25	3.8914783E-5	45.0	36
CTCCGCG	35	1.21232E-7	45.0	1
GCGAATG	35	1.21232E-7	45.0	1
CCTAGCG	20	7.034098E-4	45.0	1
TAGCGTC	20	7.034098E-4	45.0	12
CTATACG	25	3.8914783E-5	45.0	1
CGGTTCG	20	7.034098E-4	45.0	30
CGTGAAT	35	1.21232E-7	45.0	23
AGCTTCG	25	3.8914783E-5	45.0	1
TAGAACG	40	6.8175723E-9	45.0	1
TCGGGTA	25	3.8914783E-5	45.0	27
GCACGCA	20	7.034098E-4	45.0	9
CCTACGG	20	7.034098E-4	45.0	2
>>END_MODULE