FastQCFastQC Report
Thu 26 May 2016
SRR1545077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545077_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3440984
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119240.346528783627009No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG70170.2039242263259579No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG63590.18480178925563154No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG56920.16541779909467758No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA45920.13345019912908632No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39400.11450213078584498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCTT351.2128658E-745.0000048
GTTACGC351.2128658E-745.00000435
CTACCGT351.2128658E-745.00000438
TTACGCG351.2128658E-745.0000041
TCGAACG351.2128658E-745.0000041
GCATAAC207.035251E-445.09
TCACGAC1350.045.025
TATCACG800.045.01
AACCGTG207.035251E-445.01
ATAGCGG5950.045.02
CCTGTCG207.035251E-445.010
CACTGCG453.8562575E-1045.01
TAGTTCG800.045.01
ATCCGAT253.892434E-545.06
CATAGCG253.892434E-545.01
TCGTCAC900.045.018
CGGTCAG406.8212103E-945.013
TAAGCGA800.045.01
CGATATC453.8562575E-1045.010
TAGTCCG406.8212103E-945.01