##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545077_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3440984
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.865980777591528	31.0	31.0	33.0	30.0	33.0
2	31.174100489859878	31.0	31.0	33.0	30.0	34.0
3	31.350706658327965	31.0	31.0	34.0	30.0	34.0
4	35.19972658983593	37.0	35.0	37.0	33.0	37.0
5	35.06536705779509	37.0	35.0	37.0	32.0	37.0
6	35.13378673077236	37.0	35.0	37.0	32.0	37.0
7	35.51888645805967	36.0	35.0	37.0	35.0	37.0
8	35.583665021400854	37.0	35.0	37.0	35.0	37.0
9	37.420180680875006	39.0	37.0	39.0	35.0	39.0
10	36.96888273819349	39.0	37.0	39.0	33.0	39.0
11	36.493769514766704	38.0	35.0	39.0	32.0	39.0
12	34.9734195218577	35.0	34.0	39.0	30.0	39.0
13	34.464055630598686	35.0	33.0	39.0	27.0	39.0
14	35.06603024018711	35.0	33.0	40.0	27.0	41.0
15	35.55480496276646	35.0	33.0	40.0	30.0	41.0
16	35.7952109047877	35.0	34.0	40.0	31.0	41.0
17	35.652196290363456	35.0	34.0	40.0	31.0	41.0
18	35.77065048834868	36.0	35.0	39.0	31.0	41.0
19	35.63406426766297	36.0	34.0	39.0	31.0	41.0
20	35.32849411679915	35.0	34.0	39.0	30.0	41.0
21	35.19890967234954	35.0	34.0	39.0	30.0	41.0
22	35.03635152037906	35.0	34.0	39.0	29.0	41.0
23	34.98575204069534	35.0	34.0	39.0	29.0	41.0
24	35.09770722560756	35.0	34.0	39.0	30.0	41.0
25	35.03583887632142	35.0	34.0	39.0	30.0	41.0
26	34.789187918339636	35.0	34.0	39.0	29.0	41.0
27	34.75307324881487	35.0	34.0	39.0	29.0	41.0
28	34.85136722518907	35.0	34.0	39.0	29.0	41.0
29	34.85742566661165	35.0	34.0	39.0	29.0	41.0
30	34.84875024121007	36.0	34.0	39.0	29.0	41.0
31	34.568295871181036	35.0	34.0	39.0	29.0	41.0
32	34.40288068761726	35.0	34.0	39.0	28.0	41.0
33	34.174810170579114	35.0	33.0	39.0	27.0	41.0
34	34.000035164359964	35.0	33.0	39.0	25.0	41.0
35	33.80076251444354	35.0	33.0	39.0	24.0	41.0
36	33.50421187660274	35.0	33.0	39.0	23.0	41.0
37	33.54080373521062	35.0	33.0	39.0	23.0	41.0
38	33.6100275386343	35.0	33.0	39.0	24.0	41.0
39	33.6402988214999	35.0	33.0	39.0	23.0	41.0
40	33.31371375164778	35.0	32.0	39.0	23.0	41.0
41	33.422275430516386	35.0	33.0	39.0	23.0	41.0
42	33.40668366955499	35.0	33.0	39.0	23.0	41.0
43	33.38337289566008	35.0	33.0	39.0	23.0	41.0
44	33.36879421700304	35.0	33.0	38.0	23.0	41.0
45	33.445485651778675	35.0	33.0	38.0	23.0	41.0
46	33.45402855694766	35.0	33.0	38.0	23.0	41.0
47	33.36937544609333	35.0	33.0	38.0	23.0	41.0
48	33.42870702101492	35.0	33.0	38.0	23.0	41.0
49	33.50294189104047	35.0	33.0	38.0	24.0	41.0
50	33.3083350576463	35.0	33.0	38.0	24.0	41.0
51	33.00382594048679	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	3.0
11	2.0
12	7.0
13	11.0
14	27.0
15	72.0
16	238.0
17	743.0
18	1908.0
19	4056.0
20	7200.0
21	11931.0
22	18279.0
23	26535.0
24	38132.0
25	52348.0
26	66719.0
27	76631.0
28	85632.0
29	97353.0
30	116979.0
31	144116.0
32	184895.0
33	246538.0
34	458891.0
35	671620.0
36	180740.0
37	214930.0
38	305296.0
39	428933.0
40	214.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.985758724829875	25.273875147341574	30.86901886204644	12.871347265782113
2	28.906963821976504	27.557872980519527	31.102527649067824	12.432635548436146
3	28.445090125382738	26.133454848961808	32.79820539706084	12.62324962859461
4	25.707995154874304	28.51402389549036	32.421045840375896	13.356935109259444
5	22.471595334357847	32.91163225402966	31.41769912327404	13.199073288338454
6	20.61860793307961	41.56395379926207	28.26118924121705	9.556249026441273
7	85.83980628796878	5.621764007039847	7.100265505448442	1.4381641995429213
8	88.61279215480224	3.7297470723490727	6.40418554692495	1.2532752259237474
9	84.24744201077367	6.005869251353682	7.726510788774374	2.020177949098281
10	63.50709564473418	18.48119607647115	12.180033385798945	5.831674892995724
11	60.58191494061001	14.798354191707952	17.495867461168086	7.12386340651395
12	54.65192514699284	20.83903906556962	17.53079932949412	6.978236457943425
13	16.648290140262205	57.29276858015033	17.977067025013778	8.081874254573691
14	8.843400608663103	56.1231903432274	25.103080979161774	9.93032806894772
15	8.340375892477269	22.643987882535928	60.34634279031812	8.669293434668687
16	8.63540777870516	16.368457394745224	58.01843309936925	16.977701727180364
17	8.912276255861695	17.52551014477254	30.00412091425011	43.55809268511565
18	14.930467563929387	22.37275732755514	46.548981337896365	16.147793770619103
19	34.42730335276188	19.50389772227944	26.80105458206141	19.267744342897263
20	33.58533489257724	21.312624528332595	29.658754588803664	15.443285990286498
21	15.259181675939207	36.588894339526135	31.218918774397093	16.933005210137566
22	19.08974293399795	25.249725078640296	20.865979033904257	34.7945529534575
23	12.91386417373635	35.544861586104446	24.979395428749452	26.561878811409763
24	9.966945501635578	20.775423541638087	57.33516924228651	11.922461714439823
25	8.573187204590313	26.979550035687467	49.62345073385985	14.82381202586237
26	8.980977534333203	44.81520402303527	27.821402250054057	18.382416192577473
27	9.639335724897297	52.6882136040156	25.642287206217755	12.030163464869352
28	7.619651820525757	36.3202211925426	44.03336371224045	12.026763274691193
29	8.203380195897452	26.7403452035813	41.79089469756326	23.265379902957992
30	12.887418250128452	46.45342727545377	28.17653322421726	12.482621250200523
31	34.044447750992155	31.56599972565987	23.587787679338234	10.801764844009737
32	36.86085143086977	23.501242667795026	28.904871397251487	10.733034504083715
33	35.584646717334344	29.208360166743002	19.280763874519614	15.926229241403039
34	17.13849875500729	27.798937745714596	24.370180157768825	30.692383341509288
35	18.960768198864045	23.78090104458492	35.51641042213507	21.74192033441597
36	39.17629375783206	26.68463439527763	21.985106585790575	12.153965261099732
37	14.73970817649835	45.77417389909398	28.215504634720766	11.270613289686903
38	14.66513648421498	44.71345987078115	22.83117271106172	17.79023093394215
39	19.36963380242396	41.111670382657984	26.114390534800506	13.404305280117548
40	28.38295673563144	23.765411289328867	23.893950102645057	23.95768187239464
41	11.657334064907015	23.764684752966012	28.191267381655944	36.38671380047103
42	14.48501358913613	20.600299216735678	27.01404598219579	37.9006412119324
43	16.28786998137742	20.91006526040226	29.832396779525855	32.96966797869447
44	10.481187939263885	32.58538836565354	35.09658865022331	21.836835044859264
45	10.09304315277258	56.27323463288408	19.952490334160228	13.681231880183113
46	17.920339065802107	45.39739214131772	21.60585460438061	15.076414188499568
47	16.354333527851335	30.398601097825505	25.615870344064373	27.631195030258787
48	16.885954715279116	28.040874354545096	40.67008158131511	14.403089348860675
49	21.407248624230743	27.46385917516617	38.37504039542177	12.75385180518131
50	18.38168965621462	46.14459119833164	23.0068492035999	12.466869941853842
51	15.33828695512679	40.88342171890366	23.924493691339453	19.853797634630094
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5219.0
1	6642.5
2	8066.0
3	15583.0
4	23100.0
5	18926.5
6	14753.0
7	15940.5
8	17128.0
9	18359.5
10	19591.0
11	19569.0
12	19547.0
13	19775.0
14	20003.0
15	18541.0
16	17079.0
17	15383.0
18	13687.0
19	13586.5
20	13486.0
21	13258.0
22	13030.0
23	15719.5
24	18409.0
25	21890.0
26	33887.5
27	42404.0
28	55448.5
29	68493.0
30	83504.5
31	98516.0
32	106251.5
33	113987.0
34	124988.5
35	135990.0
36	134961.5
37	133933.0
38	170181.0
39	206429.0
40	305511.5
41	404594.0
42	490470.0
43	576346.0
44	541839.5
45	507333.0
46	429098.5
47	350864.0
48	294981.0
49	239098.0
50	190481.0
51	141864.0
52	109243.0
53	76622.0
54	56144.0
55	35666.0
56	28609.5
57	21553.0
58	18583.5
59	15614.0
60	14442.0
61	13270.0
62	11421.5
63	9573.0
64	8191.5
65	6810.0
66	5412.0
67	4014.0
68	3579.5
69	3145.0
70	2879.0
71	2613.0
72	2166.5
73	1720.0
74	1346.0
75	753.5
76	535.0
77	418.5
78	302.0
79	221.5
80	141.0
81	101.0
82	61.0
83	44.5
84	28.0
85	19.0
86	10.0
87	10.0
88	10.0
89	6.5
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3440984.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.096718583809276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.22902371341756	11.309659695418086
2	8.387954772351492	2.364852758391179
3	2.8124766855725096	1.1894007708012095
4	1.370652692707019	0.7728682114092504
5	0.8767893192655094	0.6179926145487796
6	0.5922114646555733	0.5008943015613079
7	0.4492556998128445	0.44331218207037776
8	0.3689234366419501	0.416048789225069
9	0.30808554771111124	0.3908695739240053
>10	2.8758410269301846	8.645757815989697
>50	0.4869699076111443	4.799900098965284
>100	0.9114794963297325	34.090845737280375
>500	0.2905716241389874	25.84170182655913
>1k	0.03893618341990708	7.711073599761842
>5k	6.213220758495811E-4	0.5566967719228335
>10k+	2.0710735861652707E-4	0.3481252521715894
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11924	0.346528783627009	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	7017	0.2039242263259579	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	6359	0.18480178925563154	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5692	0.16541779909467758	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4592	0.13345019912908632	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3940	0.11450213078584498	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.035425913052777924	0.0
2	0.0	0.0	0.0	0.11583895769349697	0.0
3	0.0	0.0	0.0	0.15853023437481836	0.0
4	0.0	0.0	0.0	0.2322010215682491	0.0
5	0.0	0.0	0.0	0.42362882245311223	0.0
6	0.0	0.0	0.0	0.5501623954078252	0.0
7	0.0	0.0	0.0	0.6518774862074337	0.0
8	0.0	0.0	0.0	0.8458626950895441	0.0
9	0.0	0.0	0.0	0.9164529681044724	0.0
10	0.0	0.0	0.0	1.081551091199494	0.0
11	0.0	0.0	0.0	1.2269164866793918	0.0
12	0.0	0.0	0.0	1.380244720696173	0.0
13	0.0	0.0	0.0	1.4367692497262412	0.0
14	0.0	0.0	0.0	1.4568507147955352	0.0
15	0.0	0.0	0.0	1.4958221252990425	0.0
16	0.0	0.0	0.0	1.5747820972140527	0.0
17	0.0	0.0	0.0	1.6691446400215753	0.0
18	0.0	0.0	0.0	1.8066634427826458	0.0
19	0.0	0.0	0.0	1.8764109336166632	0.0
20	0.0	0.0	0.0	1.940898301183615	0.0
21	0.0	0.0	0.0	2.0392132018050653	0.0
22	0.0	0.0	0.0	2.133343252976474	0.0
23	0.0	0.0	0.0	2.253454244483555	0.0
24	0.0	0.0	0.0	2.3479911560181623	0.0
25	0.0	0.0	0.0	2.4200926246678276	0.0
26	0.0	0.0	0.0	2.5015228202165427	0.0
27	0.0	0.0	0.0	2.5753389146825443	0.0
28	0.0	0.0	0.0	2.6542407636885263	0.0
29	0.0	0.0	0.0	2.7447671945001777	0.0
30	0.0	0.0	0.0	2.8755728012684743	0.0
31	0.0	0.0	0.0	2.9956256698665267	0.0
32	0.0	0.0	0.0	3.0958586264859123	0.0
33	0.0	0.0	0.0	3.1917323649281717	0.0
34	0.0	0.0	0.0	3.303793333534826	0.0
35	0.0	0.0	0.0	3.48095196025323	0.0
36	2.906145451417385E-5	0.0	0.0	3.6091129746607367	0.0
37	2.906145451417385E-5	0.0	0.0	3.7330891396182024	0.0
38	2.906145451417385E-5	0.0	0.0	3.8478528234946747	0.0
39	2.906145451417385E-5	0.0	0.0	3.961570295008637	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCTT	35	1.2128658E-7	45.000004	8
GTTACGC	35	1.2128658E-7	45.000004	35
CTACCGT	35	1.2128658E-7	45.000004	38
TTACGCG	35	1.2128658E-7	45.000004	1
TCGAACG	35	1.2128658E-7	45.000004	1
GCATAAC	20	7.035251E-4	45.0	9
TCACGAC	135	0.0	45.0	25
TATCACG	80	0.0	45.0	1
AACCGTG	20	7.035251E-4	45.0	1
ATAGCGG	595	0.0	45.0	2
CCTGTCG	20	7.035251E-4	45.0	10
CACTGCG	45	3.8562575E-10	45.0	1
TAGTTCG	80	0.0	45.0	1
ATCCGAT	25	3.892434E-5	45.0	6
CATAGCG	25	3.892434E-5	45.0	1
TCGTCAC	90	0.0	45.0	18
CGGTCAG	40	6.8212103E-9	45.0	13
TAAGCGA	80	0.0	45.0	1
CGATATC	45	3.8562575E-10	45.0	10
TAGTCCG	40	6.8212103E-9	45.0	1
>>END_MODULE