##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545076_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1419324
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.899213287452337	31.0	31.0	33.0	30.0	33.0
2	31.22140751512692	31.0	31.0	33.0	30.0	34.0
3	31.392336774408097	31.0	31.0	34.0	30.0	34.0
4	35.22057895166995	37.0	35.0	37.0	33.0	37.0
5	35.08337701610062	37.0	35.0	37.0	32.0	37.0
6	35.164570598397546	37.0	35.0	37.0	32.0	37.0
7	35.54879012825824	36.0	35.0	37.0	35.0	37.0
8	35.63186488779165	37.0	35.0	37.0	35.0	37.0
9	37.542182757425365	39.0	37.0	39.0	35.0	39.0
10	37.05199587972866	39.0	37.0	39.0	33.0	39.0
11	36.54693713345226	39.0	35.0	39.0	32.0	39.0
12	34.74425853434452	35.0	34.0	39.0	30.0	39.0
13	34.154619382184755	35.0	33.0	39.0	26.0	39.0
14	34.68161744605178	35.0	33.0	40.0	25.0	41.0
15	35.27510279541528	35.0	33.0	40.0	30.0	41.0
16	35.58414287364971	35.0	34.0	40.0	31.0	41.0
17	35.49533369406844	35.0	34.0	39.0	31.0	41.0
18	35.62663352412839	36.0	35.0	39.0	31.0	41.0
19	35.49031651687705	36.0	34.0	39.0	31.0	41.0
20	35.14185133204258	35.0	34.0	39.0	30.0	41.0
21	34.95498068094388	35.0	34.0	39.0	30.0	41.0
22	34.781010537410765	35.0	34.0	39.0	29.0	41.0
23	34.783420839780064	35.0	34.0	38.0	29.0	41.0
24	34.934279276613374	35.0	34.0	39.0	30.0	41.0
25	34.85932810267423	35.0	34.0	38.0	30.0	41.0
26	34.62713446683069	35.0	34.0	38.0	29.0	41.0
27	34.622639369164474	35.0	34.0	38.0	29.0	41.0
28	34.789974663994975	35.0	34.0	38.0	29.0	41.0
29	34.81733346297251	35.0	34.0	38.0	29.0	41.0
30	34.7741157057867	35.0	34.0	38.0	29.0	41.0
31	34.43801626689889	35.0	34.0	38.0	29.0	41.0
32	34.27719040895525	35.0	34.0	38.0	29.0	41.0
33	34.01723355625636	35.0	33.0	38.0	27.0	41.0
34	33.86213366363142	35.0	33.0	38.0	26.0	41.0
35	33.647701300055516	35.0	33.0	38.0	25.0	41.0
36	33.31320473690292	35.0	33.0	38.0	23.0	41.0
37	33.37746349670688	35.0	33.0	38.0	23.0	41.0
38	33.515141010791055	35.0	33.0	38.0	24.0	41.0
39	33.517996595562394	35.0	33.0	38.0	24.0	41.0
40	33.17538278786239	35.0	32.0	38.0	23.0	41.0
41	33.31891872468866	35.0	33.0	38.0	23.0	41.0
42	33.28554368135817	35.0	33.0	38.0	23.0	41.0
43	33.235035833960396	35.0	33.0	38.0	23.0	41.0
44	33.24089918862783	35.0	33.0	38.0	23.0	41.0
45	33.32702399170309	35.0	33.0	38.0	23.0	40.0
46	33.33890006791966	35.0	33.0	38.0	23.0	40.0
47	33.29762689843898	35.0	33.0	38.0	23.0	40.0
48	33.3847373820213	35.0	33.0	38.0	23.0	40.0
49	33.468802049426344	35.0	33.0	38.0	24.0	40.0
50	33.22991367721535	35.0	33.0	38.0	24.0	40.0
51	32.89699744385355	35.0	32.0	37.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	1.0
14	7.0
15	21.0
16	95.0
17	300.0
18	774.0
19	1685.0
20	2958.0
21	4940.0
22	7627.0
23	11023.0
24	15442.0
25	21488.0
26	26830.0
27	31754.0
28	34663.0
29	40353.0
30	48278.0
31	60148.0
32	77681.0
33	104895.0
34	203003.0
35	309152.0
36	65051.0
37	76451.0
38	110441.0
39	164178.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.424993870321366	26.34754291479606	30.688905422581453	13.538557792301123
2	27.867773672537066	26.250383985615688	33.21750354394064	12.66433879790661
3	26.501841721833774	26.031617868788242	35.02709740693457	12.439443002443417
4	25.02233457617852	28.737060741592472	33.63002387051864	12.610580811710365
5	21.036211604961235	33.07630956708969	33.44387891700556	12.44359991094352
6	19.221474448399377	40.98789282785326	30.39186260501478	9.39877011873258
7	88.58724294100571	4.46240604682229	5.723499356031462	1.226851656140529
8	91.89135109390104	2.0748609901615134	5.2083245263238	0.8254633896136472
9	89.82135157300236	2.9568301529460506	6.095296070523714	1.1265222035278766
10	69.1272042183462	16.36638286959144	9.98573969016236	4.520673221900004
11	65.88065867976586	11.980562577677825	15.605386789767522	6.533391952788792
12	58.30698276080726	20.33108719362175	15.999799904743384	5.362130140827605
13	16.83864994884889	60.841217368268275	15.049417891897834	7.2707147909850045
14	7.659914156316669	58.42105114829314	24.542035504225957	9.376999191164243
15	5.482750943406861	21.408290143758578	65.08084130191556	8.028117610919
16	6.214014559043601	13.584072417573436	61.80244961685985	18.399463406523108
17	7.002136228232596	14.420315586856841	30.06057813437947	48.5169700505311
18	14.209722374877053	20.405982002699876	48.708540121917196	16.675755500505872
19	35.773438622893714	17.735978536260923	26.68756393888922	19.803018901956143
20	36.2367577804645	18.17541308397519	29.578376748367535	16.009452387192777
21	15.095637077932874	36.204770721836596	30.292096801012313	18.407495399218217
22	18.453785041329535	23.39388328528229	18.277785762799756	39.874545910588424
23	10.29306909486488	35.97945218991576	25.02325050517007	28.704228210049294
24	8.493057258244066	17.404412241320514	62.33854990122059	11.763980599214838
25	6.860801339229098	25.15923073237682	52.872564685723624	15.107403242670456
26	7.313833909664037	45.94447779365388	28.391825967855123	18.349862328826962
27	6.245437969061328	56.81740039624498	25.238493818183866	11.698667816509833
28	5.603794482443755	35.264604840050616	46.517285693752804	12.614314983752827
29	6.167865829084832	23.959504665601372	45.09090242960733	24.781727075706463
30	12.57584596610781	47.11137132888615	28.80427583835685	11.508506866649192
31	35.99953217165355	31.133060527406002	23.787803207724238	9.079604093216208
32	41.226034365655764	19.547333801161678	28.475668698619906	10.750963134562651
33	40.52401002167229	26.32020595720216	17.648049353072306	15.507734668053242
34	18.12778477641469	24.125781005605486	23.729676944799074	34.01675727318075
35	18.19084296467896	22.25503126840665	37.96377712206656	21.59034864484783
36	42.15408180232279	24.929332555498252	21.418365362665607	11.498220279513346
37	14.291169599048562	48.12932071887744	27.64689387342143	9.93261580865257
38	14.504933334460631	46.22242701455059	22.058599727757723	17.21403992323106
39	18.25721258852806	43.68762875847939	26.0559252151024	11.99923343789015
40	30.345079770369555	22.177952320964064	23.64956838607675	23.827399522589626
41	11.847611961750806	21.695398654570766	27.76230092635649	38.69468845732194
42	14.961700076938037	17.55765420721414	25.29471776704967	42.18592794879816
43	15.022644582914118	18.658107662521033	30.240875233561894	36.078372521002954
44	9.738861598902012	30.483244135940772	37.538222421378066	22.239671843779153
45	8.806375429429785	58.63756267067984	19.265932232527597	13.290129667362773
46	17.69250713720053	46.11512241038692	21.50263082988803	14.689739622524526
47	15.403248306940487	30.469857481448916	24.72726452874749	29.39962968286311
48	16.75889367050793	25.46627831277425	43.48281294475398	14.292015071963837
49	21.386096479732604	26.14681355349448	41.0517964890328	11.415293477740107
50	18.422502543464354	48.44968449769045	22.559331061829436	10.568481897015761
51	14.315124664981358	41.88515095918902	24.234424275218345	19.565300100611278
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1668.0
1	1864.5
2	2061.0
3	4727.0
4	7393.0
5	6205.5
6	5018.0
7	5567.0
8	6116.0
9	6793.0
10	7470.0
11	7558.0
12	7646.0
13	7289.5
14	6933.0
15	6536.5
16	6140.0
17	5648.0
18	5156.0
19	5228.0
20	5300.0
21	4907.0
22	4514.0
23	5124.5
24	5735.0
25	6137.0
26	8451.0
27	10363.0
28	15522.5
29	20682.0
30	25620.5
31	30559.0
32	37334.5
33	44110.0
34	50580.0
35	57050.0
36	57776.5
37	58503.0
38	74532.5
39	90562.0
40	133554.0
41	176546.0
42	214629.0
43	252712.0
44	240602.0
45	228492.0
46	187302.5
47	146113.0
48	122941.0
49	99769.0
50	81406.0
51	63043.0
52	46277.5
53	29512.0
54	20630.0
55	11748.0
56	9104.0
57	6460.0
58	5383.0
59	4306.0
60	3900.0
61	3494.0
62	3000.5
63	2507.0
64	2057.0
65	1607.0
66	1391.5
67	1176.0
68	985.5
69	795.0
70	685.5
71	576.0
72	506.0
73	436.0
74	353.5
75	198.5
76	126.0
77	84.0
78	42.0
79	46.5
80	51.0
81	30.5
82	10.0
83	6.0
84	2.0
85	3.5
86	5.0
87	4.0
88	3.0
89	3.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1419324.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.55002623444015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.73242527320093	11.746589056950524
2	7.058860052904373	2.0541319791000037
3	2.3809872946315753	1.039302828022743
4	1.3083726899468973	0.7614742785260954
5	0.8758528121603039	0.63718406972203
6	0.6536837865739943	0.5706669746207875
7	0.5102285554649809	0.5196687207303182
8	0.417239255174145	0.4856673687057662
9	0.357135472505079	0.46766974447782705
>10	3.085126612316078	9.422337506788422
>50	0.6730011406238915	7.162222737071986
>100	1.8746847657230363	57.66521858650944
>500	0.06608531055944347	6.030571465333325
>1k	0.0063169782152409214	1.4372946834407379
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4058	0.2859107575155497	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2675	0.18847000403008757	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1474	0.10385225642629871	No Hit
ATGTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1433	0.10096355729910858	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02177092756833535	0.0
2	0.0	0.0	0.0	0.07799487643413343	0.0
3	0.0	0.0	0.0	0.10822053315522037	0.0
4	0.0	0.0	0.0	0.15387607058007896	0.0
5	0.0	0.0	0.0	0.25927836068438215	0.0
6	0.0	0.0	0.0	0.34537568588990253	0.0
7	0.0	0.0	0.0	0.4030792123574321	0.0
8	0.0	0.0	0.0	0.5352548114454487	0.0
9	0.0	0.0	0.0	0.5815444535567637	0.0
10	0.0	0.0	0.0	0.669614548897926	0.0
11	0.0	0.0	0.0	0.7680416874512092	0.0
12	0.0	0.0	0.0	0.8582959211568324	0.0
13	0.0	0.0	0.0	0.8978217799459461	0.0
14	0.0	0.0	0.0	0.9110675222852569	0.0
15	0.0	0.0	0.0	0.9331907302349569	0.0
16	0.0	0.0	0.0	0.9870191725074754	0.0
17	0.0	0.0	0.0	1.0590957385346826	0.0
18	0.0	0.0	0.0	1.1715436362662788	0.0
19	0.0	0.0	0.0	1.212760440885943	0.0
20	0.0	0.0	0.0	1.2607410288278083	0.0
21	0.0	0.0	0.0	1.3397927464060355	0.0
22	0.0	0.0	0.0	1.4092624376111444	0.0
23	0.0	0.0	0.0	1.5081123126220652	0.0
24	0.0	0.0	0.0	1.5740592000135276	0.0
25	0.0	0.0	0.0	1.622885260870668	0.0
26	0.0	0.0	0.0	1.6831252060840232	0.0
27	0.0	0.0	0.0	1.7402650839413694	0.0
28	0.0	0.0	0.0	1.8002936609259055	0.0
29	0.0	0.0	0.0	1.86941107174965	0.0
30	0.0	0.0	0.0	1.977842973133689	0.0
31	0.0	0.0	0.0	2.0787360743565246	0.0
32	0.0	0.0	0.0	2.157858248011025	0.0
33	0.0	0.0	0.0	2.228948428970411	0.0
34	0.0	0.0	0.0	2.318075365455668	0.0
35	0.0	0.0	0.0	2.4782220268240374	0.0
36	0.0	0.0	0.0	2.5753809560044076	0.0
37	0.0	0.0	0.0	2.6741603749390555	0.0
38	0.0	0.0	0.0	2.758073561780115	0.0
39	0.0	0.0	0.0	2.843748150527998	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACAGTC	35	1.2121927E-7	45.0	9
CGTATGG	145	0.0	45.0	2
CTACTCG	35	1.2121927E-7	45.0	1
CACGGTG	20	7.0338475E-4	45.0	4
CGACGGT	85	0.0	45.0	28
CAGCCTC	20	7.0338475E-4	45.0	20
CCTAGGC	20	7.0338475E-4	45.0	32
GCAACAT	35	1.2121927E-7	45.0	9
ATCAGCG	40	6.8175723E-9	45.0	1
TAGCGCC	20	7.0338475E-4	45.0	13
TAGCGAG	70	0.0	45.0	1
GCACGGT	20	7.0338475E-4	45.0	3
GCACGAT	20	7.0338475E-4	45.0	9
GCACGAG	20	7.0338475E-4	45.0	1
TTGTACC	20	7.0338475E-4	45.0	45
AGGGTGC	75	0.0	45.0	6
TGAGTCG	40	6.8175723E-9	45.0	1
CACGAAG	35	1.2121927E-7	45.0	1
TAACGCA	40	6.8175723E-9	45.0	40
ATGTCGT	20	7.0338475E-4	45.0	24
>>END_MODULE