FastQCFastQC Report
Thu 26 May 2016
SRR1545075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545075_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences929047
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32060.34508480195296903No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG22060.23744762105684641No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12330.13271664404491915No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11400.12270638622157976No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA10440.11237321685555197No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10030.10796009243881094No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCACC253.890226E-545.038
AACGTCC351.2115925E-745.033
CGTGGCG253.890226E-545.027
AACCGGG207.032588E-445.03
CAGCGCG207.032588E-445.01
AATCCGG453.8380676E-1045.02
TTGGCCA351.2115925E-745.036
ACACGTT207.032588E-445.045
ACACGTG207.032588E-445.016
AGATCGT406.8121153E-945.019
CGAACAG207.032588E-445.09
AGTTTCG253.890226E-545.01
CTCGTAG207.032588E-445.01
GATCGAG253.890226E-545.01
TAGACTC253.890226E-545.05
CCCTATA351.2115925E-745.032
ACGTTAG551.8189894E-1245.01
GCGAACA207.032588E-445.08
CATTGCG207.032588E-445.01
GATCCCC207.032588E-445.09