FastQCFastQC Report
Thu 26 May 2016
SRR1545074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545074_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1487908
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40300.2708500794403955No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG29780.20014678326885801No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG24440.1642574675315947No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA17750.1192950101753603No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15150.1018208115017864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTCG453.8562575E-1045.0000041
TCGTTAG900.045.0000041
TACGGCA453.8562575E-1045.00000442
TCGATAG650.045.0000041
ATATCGG551.8189894E-1245.0000042
TCGGACG453.8562575E-1045.0000041
AGTTGTA453.8562575E-1045.00000434
TACGACG453.8562575E-1045.0000041
TCGTCAG650.045.0000041
AACGACG551.8189894E-1245.0000041
ACTCGAT551.8189894E-1245.00000423
CTCGATT551.8189894E-1245.00000424
AATTACG453.8562575E-1045.0000041
TTACGAG650.045.0000041
TCGAGAG551.8189894E-1245.0000041
TGAATCG453.8562575E-1045.0000041
GCGTCAG551.8189894E-1245.0000041
AATCGTG551.8189894E-1245.0000041
ACGCGAG406.8175723E-945.01
TCACGAC351.2122473E-745.025