FastQCFastQC Report
Thu 26 May 2016
SRR1545073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545073_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1580028
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66090.418283726617503No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG53930.3413230651608706No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG50580.3201209092497095No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG39690.2511980800340247No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA31170.19727498500026583No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA19620.12417501461999408No Hit
TAGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA17450.11044108079097331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC302.1660198E-645.00000441
AGTTTCG302.1660198E-645.0000041
AGTCACG302.1660198E-645.0000041
CATGCGT302.1660198E-645.00000435
CCATGCG302.1660198E-645.00000434
CTACGTG302.1660198E-645.0000041
CGGATAT302.1660198E-645.00000422
GTACTCG302.1660198E-645.0000041
ACGCTCT302.1660198E-645.00000418
CACGTAG302.1660198E-645.0000041
TATACGT302.1660198E-645.00000425
TGATCGC253.8914724E-545.028
TTTAGCG453.8562575E-1045.01
AACGTAG253.8914724E-545.01
GATCGAC207.034091E-445.09
GTACAAC207.034091E-445.030
GGTCGAA207.034091E-445.09
CACGGAT253.8914724E-545.04
CGCGGCT207.034091E-445.019
ACGTTGT207.034091E-445.032