##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545073_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1580028
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.03400192907974	31.0	31.0	33.0	30.0	33.0
2	31.36337330730848	31.0	31.0	33.0	30.0	34.0
3	31.507935302412363	31.0	31.0	34.0	30.0	34.0
4	35.35539053738288	37.0	35.0	37.0	33.0	37.0
5	35.25110061340685	37.0	35.0	37.0	33.0	37.0
6	35.30803378167982	37.0	35.0	37.0	32.0	37.0
7	35.64698726858005	36.0	35.0	37.0	35.0	37.0
8	35.710488041984064	37.0	35.0	37.0	35.0	37.0
9	37.58004731561719	39.0	37.0	39.0	35.0	39.0
10	37.18191449771776	39.0	37.0	39.0	33.0	39.0
11	36.687758697947125	39.0	35.0	39.0	32.0	39.0
12	35.121421898852425	35.0	35.0	39.0	31.0	39.0
13	34.58339535755063	35.0	33.0	39.0	30.0	39.0
14	35.18801059221735	35.0	33.0	40.0	27.0	41.0
15	35.66031804499667	35.0	34.0	40.0	31.0	41.0
16	35.891482935745444	35.0	34.0	40.0	32.0	41.0
17	35.79021511011197	35.0	34.0	40.0	31.0	41.0
18	35.93640619027005	36.0	35.0	39.0	31.0	41.0
19	35.80716924003878	36.0	35.0	39.0	31.0	41.0
20	35.51102828557469	35.0	34.0	39.0	31.0	41.0
21	35.34939697271188	35.0	34.0	39.0	31.0	41.0
22	35.181401848574836	35.0	34.0	39.0	30.0	41.0
23	35.15465548711795	35.0	34.0	39.0	30.0	41.0
24	35.206745070340524	35.0	34.0	39.0	31.0	41.0
25	35.14700562268517	35.0	34.0	39.0	31.0	41.0
26	34.933102451348965	35.0	34.0	39.0	30.0	41.0
27	34.891979762383954	35.0	34.0	39.0	30.0	41.0
28	35.02970263818109	35.0	34.0	39.0	30.0	41.0
29	35.05220477105469	36.0	34.0	39.0	30.0	41.0
30	35.02771976192827	36.0	34.0	39.0	30.0	41.0
31	34.74656018754098	35.0	34.0	39.0	29.0	41.0
32	34.56853929170875	35.0	34.0	39.0	29.0	41.0
33	34.332050444675666	35.0	33.0	39.0	27.0	41.0
34	34.15973767553486	35.0	34.0	39.0	27.0	41.0
35	33.949628740756495	35.0	33.0	39.0	25.0	41.0
36	33.66521226206118	35.0	33.0	39.0	23.0	41.0
37	33.6698640783581	35.0	33.0	39.0	23.0	41.0
38	33.772837569967116	35.0	33.0	39.0	25.0	41.0
39	33.784715840478775	35.0	33.0	39.0	24.0	41.0
40	33.41560592597093	35.0	33.0	39.0	23.0	41.0
41	33.55857807583157	35.0	33.0	39.0	23.0	41.0
42	33.5504655613698	35.0	33.0	39.0	23.0	41.0
43	33.52472551119347	35.0	33.0	39.0	23.0	41.0
44	33.4837578827717	35.0	33.0	38.0	23.0	41.0
45	33.53934107496829	35.0	33.0	38.0	23.0	41.0
46	33.59096927396223	35.0	33.0	38.0	23.0	41.0
47	33.55917869809902	35.0	33.0	38.0	23.0	41.0
48	33.59759067560828	35.0	33.0	38.0	23.0	41.0
49	33.65923262119406	35.0	33.0	38.0	24.0	41.0
50	33.45829757447336	35.0	33.0	38.0	24.0	41.0
51	33.182437906163685	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	3.0
13	7.0
14	4.0
15	23.0
16	97.0
17	289.0
18	666.0
19	1502.0
20	2706.0
21	4554.0
22	7329.0
23	10811.0
24	15980.0
25	22576.0
26	28404.0
27	33029.0
28	36067.0
29	41174.0
30	49816.0
31	62206.0
32	81039.0
33	110955.0
34	217729.0
35	332226.0
36	79918.0
37	95824.0
38	140093.0
39	204885.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.470852415273654	25.417967276529275	30.734898368889663	13.376281939307406
2	30.562622940859274	27.01926801297192	30.859896153739047	11.558212892429754
3	28.076274597665357	25.710177287997425	33.681301850346955	12.532246263990258
4	26.58497191189017	27.192113051161122	33.05631292610005	13.166602110848668
5	23.008516304774346	33.57750622140874	31.663236347710296	11.750741126106625
6	20.966527175467778	41.10629685043556	28.56405076365735	9.363125210439309
7	85.75468282840558	5.7040128402787795	7.138101350102656	1.4032029812129911
8	88.63893551253523	3.732528790629027	6.542099253937272	1.0864364428984803
9	84.71704298911159	6.079322644915154	7.675939920052049	1.5276944459212116
10	64.61132334363695	19.12466108195551	11.182080317563994	5.08193525684355
11	62.73547051064918	14.238671719741674	16.851789968279043	6.174067801330103
12	55.23161614857458	21.182536005691038	16.943560493864666	6.642287351869713
13	17.130645786024047	57.404425744353894	17.41956471657464	8.045363753047415
14	8.963385458991866	57.307085697215484	24.944811104613336	8.784717739179305
15	9.160723734009776	21.986255939768157	60.78734047751052	8.065679848711541
16	9.072370869376998	16.34477363692289	58.91300660494624	15.66984888875387
17	8.65389727270656	17.380957805811036	30.348829261253595	43.61631566022881
18	15.08751743639986	23.32401704273595	45.80602369071941	15.782441830144784
19	35.70037999326594	17.866961851308965	28.138678555063578	18.293979600361514
20	35.31912092697092	20.546091588250334	28.639112724584624	15.495674760194122
21	16.504707511512454	37.16016425025379	30.13756718235373	16.197561055880023
22	18.989536894282885	26.626933193588975	18.96744867812469	35.41608123400345
23	12.991225471953664	36.04644980975021	24.66855017759179	26.29377454070434
24	9.901027070406347	21.127220530269085	57.29050371259243	11.681248686732133
25	8.424344378707213	26.04960165262894	50.414866065664654	15.111187902999188
26	8.547696623097819	45.62963441154208	29.11904092838861	16.703628036971494
27	9.80064910242097	53.900057467336026	25.482839544615665	10.816453885627343
28	7.884100788087299	35.88626277509006	44.98667112228391	11.242965314538731
29	8.15884275468536	25.9973873880716	43.963081666907165	21.880688190335867
30	12.770786340495233	46.04469034725967	28.783793704921685	12.400729607323415
31	35.402347300174426	30.309209710207668	25.125883845096414	9.16255914452149
32	38.87772874911077	22.267769938254258	28.910943350371003	9.94355796226396
33	38.06983167386907	28.225765619343456	19.503261967509435	14.201140739278037
34	18.754604348783694	27.28337725660558	23.97698015478207	29.98503823982866
35	19.49921140638014	23.383383079287203	36.96016779449478	20.157237719837877
36	40.24827408121882	26.564908976296625	22.225428916449584	10.961388026034982
37	15.279476059917926	47.830101745032366	26.71167852721597	10.178743667833734
38	15.16492112798001	47.413400268855995	21.140574723992234	16.281103879171763
39	20.243058983764843	42.40000810112226	24.76051057323035	12.59642234188255
40	29.345745771593922	24.943545304260432	23.65293526443835	22.057773659707298
41	12.558258461242458	24.38709946912333	27.198505342943292	35.85613672669092
42	15.274539438541595	21.559238190715607	25.10252982858532	38.063692542157476
43	17.39703347029293	20.768239550185186	28.17120962413324	33.66351735538864
44	10.98809641348128	32.17999934178381	35.63430521484429	21.19759902989061
45	10.635760885250136	55.84578247980415	20.20951527441286	13.30894136053285
46	17.377793304928772	46.67993225436511	22.220808745161477	13.721465695544635
47	18.13372927568372	29.538084135217858	24.648487241998243	27.67969934710018
48	18.13391914573666	27.244137445665515	40.94193267461083	13.680010733986991
49	22.02714129116699	27.736217332857393	39.156837726926355	11.079803649049257
50	17.258744781738045	47.700736948965464	23.35926958256436	11.681248686732133
51	16.140283589911064	42.96512466867676	22.76225484611665	18.132336895295527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1852.0
1	2943.0
2	4034.0
3	8390.0
4	12746.0
5	10308.5
6	7871.0
7	8299.5
8	8728.0
9	9017.5
10	9307.0
11	9505.0
12	9703.0
13	9490.0
14	9277.0
15	8340.0
16	7403.0
17	7196.0
18	6989.0
19	6992.0
20	6995.0
21	6443.0
22	5891.0
23	6770.5
24	7650.0
25	10580.0
26	18830.0
27	24150.0
28	28566.0
29	32982.0
30	38650.5
31	44319.0
32	48540.0
33	52761.0
34	55469.0
35	58177.0
36	56811.0
37	55445.0
38	74719.0
39	93993.0
40	136818.0
41	179643.0
42	220276.5
43	260910.0
44	246966.0
45	233022.0
46	202657.0
47	172292.0
48	137139.5
49	101987.0
50	82576.5
51	63166.0
52	49537.5
53	35909.0
54	26264.0
55	16619.0
56	13399.0
57	10179.0
58	9390.5
59	8602.0
60	7926.0
61	7250.0
62	6256.5
63	5263.0
64	4383.5
65	3504.0
66	2987.0
67	2470.0
68	2014.0
69	1558.0
70	1452.0
71	1346.0
72	1296.0
73	1246.0
74	947.0
75	483.0
76	318.0
77	231.0
78	144.0
79	122.5
80	101.0
81	72.0
82	43.0
83	24.5
84	6.0
85	6.0
86	6.0
87	7.0
88	8.0
89	6.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1580028.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.319338315082343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.94053852957606	12.246361548244508
2	7.123249757017409	2.182469458611556
3	2.503643658127223	1.150624926577838
4	1.4985950810222697	0.9182994017399354
5	1.0188568254634427	0.7804106201952641
6	0.7659003231467734	0.7039851699549787
7	0.5991791212113459	0.6425319368419237
8	0.45502639240341214	0.5576562598015428
9	0.4181790979150603	0.5765604369531078
>10	3.3333389018887853	10.16982798634863
>50	0.6509240493270692	7.2242645690472616
>100	1.6254456047395456	54.14418564406819
>500	0.05759289805220413	5.654876161940541
>1k	0.008286747921180452	1.9650837478675183
>5k	0.0012430121881770676	1.0828621318072247
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6609	0.418283726617503	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5393	0.3413230651608706	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5058	0.3201209092497095	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3969	0.2511980800340247	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3117	0.19727498500026583	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1962	0.12417501461999408	No Hit
TAGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1745	0.11044108079097331	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008987182505626483	0.0
2	0.0	0.0	0.0	0.035442409881343874	0.0
3	0.0	0.0	0.0	0.05379651499846838	0.0
4	0.0	0.0	0.0	0.09411225623849703	0.0
5	0.0	0.0	0.0	0.1796803600948844	0.0
6	0.0	0.0	0.0	0.26594465414536955	0.0
7	0.0	0.0	0.0	0.32822203150830237	0.0
8	0.0	0.0	0.0	0.46018171829866306	0.0
9	0.0	0.0	0.0	0.5144845534382935	0.0
10	0.0	0.0	0.0	0.5998627872417451	0.0
11	0.0	0.0	0.0	0.6974559944507313	0.0
12	0.0	0.0	0.0	0.7800494674777916	0.0
13	0.0	0.0	0.0	0.8135931768297777	0.0
14	0.0	0.0	0.0	0.824732219935343	0.0
15	0.0	0.0	0.0	0.842959745017177	0.0
16	0.0	0.0	0.0	0.8909335783922817	0.0
17	0.0	0.0	0.0	0.946945244008334	0.0
18	0.0	0.0	0.0	1.0264375061707767	0.0
19	0.0	0.0	0.0	1.0663102172872885	0.0
20	0.0	0.0	0.0	1.1093474292860632	0.0
21	0.0	0.0	0.0	1.1746627274959685	0.0
22	0.0	0.0	0.0	1.2357375945236413	0.0
23	0.0	0.0	0.0	1.3153564367213746	0.0
24	0.0	0.0	0.0	1.3733933829020752	0.0
25	0.0	0.0	0.0	1.4216203763477608	0.0
26	0.0	0.0	0.0	1.4783915221755564	0.0
27	0.0	0.0	0.0	1.5267450956565327	0.0
28	0.0	0.0	0.0	1.5847820418372334	0.0
29	0.0	0.0	0.0	1.6430088580708697	0.0
30	0.0	0.0	0.0	1.7336401633388776	0.0
31	0.0	0.0	0.0	1.8188285270893934	0.0
32	0.0	0.0	0.0	1.8922449475578913	0.0
33	0.0	0.0	0.0	1.9651550478852273	0.0
34	0.0	0.0	0.0	2.0439511198535723	0.0
35	0.0	0.0	0.0	2.1703412850911503	0.0
36	0.0	0.0	0.0	2.2671750120883933	0.0
37	0.0	0.0	0.0	2.364388479191508	0.0
38	0.0	0.0	0.0	2.447235112289149	0.0
39	6.329001764525692E-5	0.0	0.0	2.5318538658808576	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	30	2.1660198E-6	45.000004	41
AGTTTCG	30	2.1660198E-6	45.000004	1
AGTCACG	30	2.1660198E-6	45.000004	1
CATGCGT	30	2.1660198E-6	45.000004	35
CCATGCG	30	2.1660198E-6	45.000004	34
CTACGTG	30	2.1660198E-6	45.000004	1
CGGATAT	30	2.1660198E-6	45.000004	22
GTACTCG	30	2.1660198E-6	45.000004	1
ACGCTCT	30	2.1660198E-6	45.000004	18
CACGTAG	30	2.1660198E-6	45.000004	1
TATACGT	30	2.1660198E-6	45.000004	25
TGATCGC	25	3.8914724E-5	45.0	28
TTTAGCG	45	3.8562575E-10	45.0	1
AACGTAG	25	3.8914724E-5	45.0	1
GATCGAC	20	7.034091E-4	45.0	9
GTACAAC	20	7.034091E-4	45.0	30
GGTCGAA	20	7.034091E-4	45.0	9
CACGGAT	25	3.8914724E-5	45.0	4
CGCGGCT	20	7.034091E-4	45.0	19
ACGTTGT	20	7.034091E-4	45.0	32
>>END_MODULE