FastQCFastQC Report
Thu 26 May 2016
SRR1545072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545072_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2374616
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74340.31306114335960006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA302.1664837E-645.00000411
CTCGTAG650.045.0000041
CATTGCG302.1664837E-645.0000041
ATGTGCG1250.045.0000041
TAGCGAT302.1664837E-645.00000412
GAATCGG600.045.0000042
TGTATCG650.045.0000041
ATTCGCA600.045.00000418
CAATTCG600.045.00000416
ATAGACG650.045.0000041
TTATCCG302.1664837E-645.0000041
TCGTGCA302.1664837E-645.0000041
TGCGATG302.1664837E-645.0000041
TCCGTTG302.1664837E-645.00000433
GCTAGGT302.1664837E-645.00000416
TATAGCG600.045.0000041
ACGTAAC302.1664837E-645.00000426
GTAAGCG1200.045.0000041
GCGTATT650.045.00000412
CCGTTGA302.1664837E-645.00000434