##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545072_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2374616
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.94501468869072	31.0	31.0	33.0	30.0	33.0
2	31.262584771600967	31.0	31.0	33.0	30.0	34.0
3	31.42178609088796	31.0	31.0	34.0	30.0	34.0
4	35.25922633385777	37.0	35.0	37.0	33.0	37.0
5	35.12574917376115	37.0	35.0	37.0	32.0	37.0
6	35.17734698999754	37.0	35.0	37.0	32.0	37.0
7	35.589703766840614	36.0	35.0	37.0	35.0	37.0
8	35.67668077701826	37.0	35.0	37.0	35.0	37.0
9	37.5575520420986	39.0	37.0	39.0	35.0	39.0
10	37.111996634403205	39.0	37.0	39.0	33.0	39.0
11	36.60550800634713	39.0	35.0	39.0	32.0	39.0
12	34.721857765634525	35.0	34.0	39.0	30.0	39.0
13	34.09033376343796	35.0	33.0	39.0	25.0	39.0
14	34.617157468828644	35.0	33.0	39.0	25.0	41.0
15	35.219592136160124	35.0	33.0	40.0	30.0	41.0
16	35.543167821660425	35.0	34.0	40.0	31.0	41.0
17	35.429606723781866	35.0	34.0	39.0	31.0	41.0
18	35.564839957281514	36.0	35.0	39.0	31.0	41.0
19	35.42391485612832	36.0	34.0	39.0	31.0	41.0
20	35.08250260252605	35.0	34.0	39.0	30.0	41.0
21	34.89325768882211	35.0	34.0	39.0	30.0	41.0
22	34.716340241959124	35.0	34.0	38.0	29.0	41.0
23	34.74907058657063	35.0	34.0	38.0	29.0	41.0
24	34.877561256219956	35.0	34.0	38.0	30.0	41.0
25	34.80122301879546	35.0	34.0	38.0	30.0	41.0
26	34.558147506796885	35.0	34.0	38.0	29.0	41.0
27	34.5198478406614	35.0	34.0	38.0	29.0	41.0
28	34.683798559430244	35.0	34.0	38.0	29.0	41.0
29	34.736582672735295	35.0	34.0	38.0	29.0	41.0
30	34.69650798276437	35.0	34.0	38.0	29.0	41.0
31	34.358581345362786	35.0	34.0	38.0	29.0	41.0
32	34.17183325640862	35.0	34.0	38.0	29.0	41.0
33	33.941254922901216	35.0	33.0	38.0	27.0	41.0
34	33.779962739238684	35.0	33.0	38.0	26.0	41.0
35	33.54838171729661	35.0	33.0	38.0	24.0	41.0
36	33.23556524507541	35.0	33.0	38.0	23.0	41.0
37	33.253482668355645	35.0	33.0	38.0	23.0	40.0
38	33.37110758118365	35.0	33.0	38.0	23.0	41.0
39	33.39418415440644	35.0	33.0	38.0	23.0	41.0
40	32.9800245597604	35.0	32.0	38.0	23.0	41.0
41	33.179388162128106	35.0	32.0	38.0	23.0	41.0
42	33.16876412860016	35.0	33.0	38.0	23.0	41.0
43	33.13817518285062	35.0	33.0	38.0	23.0	41.0
44	33.1250669581945	35.0	33.0	38.0	23.0	40.0
45	33.21018766823773	35.0	33.0	38.0	23.0	40.0
46	33.23789614826145	35.0	33.0	38.0	23.0	40.0
47	33.214362659057294	35.0	33.0	38.0	23.0	40.0
48	33.29044022275602	35.0	33.0	38.0	23.0	40.0
49	33.366396503687334	35.0	33.0	37.0	24.0	40.0
50	33.09130444669791	35.0	32.0	37.0	24.0	40.0
51	32.780121080629456	35.0	32.0	37.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	2.0
12	5.0
13	4.0
14	14.0
15	56.0
16	160.0
17	507.0
18	1332.0
19	2836.0
20	5098.0
21	8378.0
22	12776.0
23	18745.0
24	26342.0
25	36460.0
26	45980.0
27	53855.0
28	59405.0
29	67974.0
30	81210.0
31	100315.0
32	130548.0
33	178113.0
34	348120.0
35	527470.0
36	104920.0
37	122414.0
38	177258.0
39	264139.0
40	176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.520724192880028	24.956456117536476	31.577316079736683	12.945503609846812
2	29.354725142928373	25.780926263446386	32.71897435206366	12.145374241561584
3	26.86139569513555	26.902539189494217	33.08846567192338	13.147599443446856
4	25.30181722013159	28.594728579273447	33.31317568819548	12.790278512399478
5	23.610680632152736	31.688281389496236	32.56278067695998	12.138257301391047
6	20.253969483908136	41.765826558904685	28.91166403325843	9.068539923928753
7	87.03920970801174	5.351433663379678	6.375978263432909	1.2333783651756747
8	90.23555808602318	2.8006633493583806	6.017941427161276	0.9458371374571721
9	87.68588268587426	3.610267933846988	7.023240810303645	1.6806085699751034
10	70.77476947851778	14.32930629626011	10.160042718485853	4.735881506736247
11	68.23722235510921	11.761017360280569	14.591875065273713	5.409885219336516
12	59.34142615058603	19.80686561532475	15.17740131457044	5.674306919518777
13	16.617213056763706	61.103353131622114	15.070099755076189	7.209334056537983
14	7.650289562607175	59.6403797498206	25.047839313809057	7.661491373763169
15	6.077403672846473	20.33545634325718	66.42808774134429	7.159052242552059
16	6.749722902566141	12.859342310504099	63.57625822448767	16.814676562442095
17	7.642498829284398	13.290612040009838	30.61695027743433	48.44993885327143
18	14.799992925171901	20.47417350847463	48.47870139845769	16.247132167895778
19	37.75755743244382	15.85561623437221	27.86079938819582	18.526026944988157
20	36.816015726332175	18.856606710306004	28.00153793286999	16.325839630491835
21	15.632759149268766	35.9274088947434	31.157206049314922	17.282625906672912
22	18.399522280655063	23.437431567882978	18.996039780747708	39.167006370714255
23	10.499634467214909	36.32439939762892	24.476589056925416	28.699377078230754
24	8.652388428276403	17.564566228813415	62.1995724782449	11.583472864665277
25	7.429159072456347	22.41688761467117	54.58701533216318	15.566937980709303
26	6.860646100253683	46.102906743658764	29.623358050312138	17.413089105775416
27	6.966010504435244	57.221293884990246	25.764376219144484	10.048319391430024
28	6.0804778541035684	35.76355082253299	47.47049628234628	10.685475041017158
29	6.776295620007613	25.418172875109068	45.330992463623595	22.47453904125972
30	13.509047357551704	46.718837908950334	28.69310237950052	11.079012353997445
31	37.636611561616704	28.920423344237552	24.808642744763784	8.634322349381964
32	42.3600278950365	18.970309304746536	29.929681262149334	8.739981538067628
33	41.76755315385729	25.076349186563217	18.373075899429633	14.78302176014985
34	19.726894790568245	24.84885977353812	23.18656995488955	32.23767548100408
35	18.87589403928888	22.277328208013422	37.8551311033026	20.9916466493951
36	44.55554076953916	23.58233920768663	21.768530153928044	10.093589868846163
37	15.241074767457139	48.556903516189564	26.74331344520546	9.45870827114784
38	15.415924090463468	48.10853628544573	19.324092821744653	17.15144680234615
39	20.418501349270787	42.26990806092438	24.989556206140275	12.322034383664558
40	31.102797252271525	21.5033083243775	22.938277178289038	24.45561724506194
41	11.75288973038167	21.6539432059752	27.072082391426655	39.521084672216475
42	15.686494153159922	16.557456026574403	26.109231976875417	41.64681784339026
43	16.449859682576047	17.77672684762505	29.910183372806383	35.863230096992524
44	9.76983225919475	31.539962671859367	37.19611928833967	21.494085780606213
45	9.971506972074643	58.98065202963342	19.33104131362713	11.716799684664805
46	18.255119985715584	45.92131948913003	21.645941912292347	14.177618612862036
47	17.07905615055234	29.441223338847205	23.963706131854583	29.51601437874587
48	18.716878855360193	25.453673351817724	42.87143689758681	12.958010895235272
49	23.3710208303153	25.34144467989772	40.846604250961	10.440930238825983
50	18.753221573509148	49.03626523193645	21.52187974813612	10.688633446418285
51	15.454540860501234	43.41464051450845	22.71390405859305	18.41691456639726
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3128.0
1	4164.5
2	5201.0
3	9969.5
4	14738.0
5	12197.0
6	9656.0
7	10222.0
8	10788.0
9	11890.0
10	12992.0
11	13136.5
12	13281.0
13	12611.5
14	11942.0
15	11685.5
16	11429.0
17	10340.0
18	9251.0
19	8826.0
20	8401.0
21	8091.0
22	7781.0
23	7536.0
24	7291.0
25	12217.0
26	16908.5
27	16674.0
28	24648.5
29	32623.0
30	39609.5
31	46596.0
32	56177.0
33	65758.0
34	71151.5
35	76545.0
36	76795.5
37	77046.0
38	104948.0
39	132850.0
40	216871.0
41	300892.0
42	371652.5
43	442413.0
44	416075.0
45	389737.0
46	320321.5
47	250906.0
48	208799.5
49	166693.0
50	134516.5
51	102340.0
52	77469.0
53	52598.0
54	38608.5
55	24619.0
56	19063.0
57	13507.0
58	11734.0
59	9961.0
60	9338.0
61	8715.0
62	7998.5
63	7282.0
64	6047.0
65	4812.0
66	3906.5
67	3001.0
68	2545.0
69	2089.0
70	1749.0
71	1409.0
72	1267.0
73	1125.0
74	886.0
75	533.0
76	419.0
77	297.5
78	176.0
79	129.5
80	83.0
81	59.5
82	36.0
83	29.5
84	23.0
85	16.5
86	10.0
87	7.0
88	4.0
89	3.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2374616.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.42223695635968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.97248567716373	10.86831889704332
2	7.703150888183361	2.0678703306357917
3	2.6013523826171956	1.047479042594365
4	1.318714037390204	0.708003691501163
5	0.8275398462752812	0.5553717953768144
6	0.6210054826974021	0.5001169643977832
7	0.4744801984769147	0.44580099585404037
8	0.36079176465729124	0.3874106045706655
9	0.3181540204932746	0.3843304786511321
>10	2.8808438284018814	8.158650865679872
>50	0.4082558335207283	3.9105215565124745
>100	1.2610217001023616	46.79423292791129
>500	0.22193981921800926	18.548063484400014
>1k	0.02994926537743023	5.309263634426789
>5k	3.152554250255813E-4	0.3145647304444717
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7434	0.31306114335960006	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02097181186347603	0.0
2	0.0	0.0	0.0	0.07340134152216611	0.0
3	0.0	0.0	0.0	0.10367992130095982	0.0
4	0.0	0.0	0.0	0.15219302826225378	0.0
5	0.0	0.0	0.0	0.25882079460426444	0.0
6	0.0	0.0	0.0	0.347719378627955	0.0
7	0.0	0.0	0.0	0.4145512369157792	0.0
8	0.0	0.0	0.0	0.5600905577996611	0.0
9	0.0	0.0	0.0	0.611888406378126	0.0
10	0.0	0.0	0.0	0.6976706970727057	0.0
11	0.0	0.0	0.0	0.7945284627072335	0.0
12	0.0	0.0	0.0	0.8777419170088975	0.0
13	0.0	0.0	0.0	0.914463643805988	0.0
14	0.0	0.0	0.0	0.9271393774825066	0.0
15	0.0	0.0	0.0	0.9503431291627783	0.0
16	0.0	0.0	0.0	1.0010039517968379	0.0
17	0.0	0.0	0.0	1.0648458529715963	0.0
18	0.0	0.0	0.0	1.164651463647175	0.0
19	0.0	0.0	0.0	1.2052053889976315	0.0
20	0.0	0.0	0.0	1.2499705215495895	0.0
21	0.0	0.0	0.0	1.3223190612713802	0.0
22	0.0	0.0	0.0	1.3894035919912946	0.0
23	0.0	0.0	0.0	1.47800739151088	0.0
24	0.0	0.0	0.0	1.540164809805038	0.0
25	0.0	0.0	0.0	1.5873724425338664	0.0
26	0.0	0.0	0.0	1.6445185242582379	0.0
27	0.0	0.0	0.0	1.693115855363562	0.0
28	0.0	0.0	0.0	1.7465139626785973	0.0
29	0.0	0.0	0.0	1.8058498721477494	0.0
30	0.0	0.0	0.0	1.9076347502080335	0.0
31	0.0	0.0	0.0	2.006429671155252	0.0
32	0.0	0.0	0.0	2.0811785989818987	0.0
33	0.0	0.0	0.0	2.15630653545668	0.0
34	0.0	0.0	0.0	2.2445734384001454	0.0
35	0.0	0.0	0.0	2.3925552594608983	0.0
36	0.0	0.0	0.0	2.492739878784612	0.0
37	0.0	0.0	0.0	2.590818894507575	0.0
38	0.0	0.0	0.0	2.677274978354395	0.0
39	0.0	0.0	0.0	2.765162872649725	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCGA	30	2.1664837E-6	45.000004	11
CTCGTAG	65	0.0	45.000004	1
CATTGCG	30	2.1664837E-6	45.000004	1
ATGTGCG	125	0.0	45.000004	1
TAGCGAT	30	2.1664837E-6	45.000004	12
GAATCGG	60	0.0	45.000004	2
TGTATCG	65	0.0	45.000004	1
ATTCGCA	60	0.0	45.000004	18
CAATTCG	60	0.0	45.000004	16
ATAGACG	65	0.0	45.000004	1
TTATCCG	30	2.1664837E-6	45.000004	1
TCGTGCA	30	2.1664837E-6	45.000004	1
TGCGATG	30	2.1664837E-6	45.000004	1
TCCGTTG	30	2.1664837E-6	45.000004	33
GCTAGGT	30	2.1664837E-6	45.000004	16
TATAGCG	60	0.0	45.000004	1
ACGTAAC	30	2.1664837E-6	45.000004	26
GTAAGCG	120	0.0	45.000004	1
GCGTATT	65	0.0	45.000004	12
CCGTTGA	30	2.1664837E-6	45.000004	34
>>END_MODULE