FastQCFastQC Report
Thu 26 May 2016
SRR1545071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545071_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2197948
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104870.4771268474049431No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG34570.15728306584141208No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG33550.15264237370492842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAT302.166409E-645.00000424
ACCGGGT750.045.0000044
GTCGATG302.166409E-645.0000041
CGACACT302.166409E-645.00000434
ACTCGTT351.2126111E-745.00000438
TCGGTAT302.166409E-645.0000041
TATCCGT351.2126111E-745.00000415
ACGTTAT453.8562575E-1045.017
CGGCCTT207.0346944E-445.044
CGCATGA1350.045.038
TACGGGT950.045.04
ATGTACG406.8193913E-945.01
ACGATTT207.0346944E-445.031
TACGCTA207.0346944E-445.016
GGACGTC453.8562575E-1045.08
ACCGTAA207.0346944E-445.018
GCTACCT453.8562575E-1045.01
CGGTAGT850.045.012
CTTAACG253.8919727E-544.9999961
CAAGTCG253.8919727E-544.9999961
Produced by FastQC (version 0.11.5)