##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545071_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2197948
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.883834831397284	31.0	31.0	33.0	30.0	33.0
2	31.19797920605947	31.0	31.0	33.0	30.0	34.0
3	31.32934810104698	31.0	31.0	34.0	28.0	34.0
4	35.19775490593954	37.0	35.0	37.0	33.0	37.0
5	35.070416133593696	37.0	35.0	37.0	32.0	37.0
6	35.137715723938875	37.0	35.0	37.0	32.0	37.0
7	35.535090912068895	36.0	35.0	37.0	35.0	37.0
8	35.60795341837022	37.0	35.0	37.0	35.0	37.0
9	37.44348046450598	39.0	37.0	39.0	35.0	39.0
10	36.997750629223255	39.0	37.0	39.0	33.0	39.0
11	36.53033374765918	39.0	35.0	39.0	32.0	39.0
12	34.96268519546413	35.0	34.0	39.0	30.0	39.0
13	34.42159323150502	35.0	33.0	39.0	27.0	39.0
14	35.00751610138183	35.0	33.0	40.0	27.0	41.0
15	35.54027301828797	35.0	33.0	40.0	30.0	41.0
16	35.76467778127599	35.0	34.0	40.0	31.0	41.0
17	35.61464556941293	35.0	34.0	40.0	31.0	41.0
18	35.717481487278135	36.0	35.0	39.0	31.0	41.0
19	35.60296922402168	36.0	34.0	39.0	31.0	41.0
20	35.28395803722381	35.0	34.0	39.0	30.0	41.0
21	35.11908198010144	35.0	34.0	39.0	30.0	41.0
22	34.93963687948941	35.0	34.0	39.0	29.0	41.0
23	34.90691317537995	35.0	34.0	39.0	29.0	41.0
24	35.02713439990391	35.0	34.0	39.0	30.0	41.0
25	34.92469658062884	35.0	34.0	39.0	30.0	41.0
26	34.67108685009836	35.0	34.0	39.0	29.0	41.0
27	34.66141646663161	35.0	34.0	39.0	29.0	41.0
28	34.76261813291306	35.0	34.0	39.0	29.0	41.0
29	34.76068223634044	35.0	34.0	39.0	29.0	41.0
30	34.71565432849185	35.0	34.0	39.0	29.0	41.0
31	34.41430779982056	35.0	34.0	39.0	29.0	41.0
32	34.2490691317538	35.0	34.0	39.0	27.0	41.0
33	33.99347573281988	35.0	33.0	39.0	26.0	41.0
34	33.797076636935905	35.0	33.0	39.0	24.0	41.0
35	33.56754345416725	35.0	33.0	38.0	23.0	41.0
36	33.283363846642416	35.0	33.0	38.0	23.0	41.0
37	33.34120688933496	35.0	33.0	38.0	23.0	41.0
38	33.41915641316355	35.0	33.0	38.0	23.0	41.0
39	33.39988389170262	35.0	33.0	38.0	23.0	41.0
40	33.081959172828476	35.0	32.0	38.0	23.0	41.0
41	33.18249385335777	35.0	32.0	38.0	23.0	41.0
42	33.14754898660023	35.0	32.0	38.0	23.0	41.0
43	33.12700027480177	35.0	32.0	38.0	23.0	41.0
44	33.12004378629522	35.0	32.0	38.0	23.0	41.0
45	33.19095447208032	35.0	33.0	38.0	23.0	41.0
46	33.22141515631853	35.0	33.0	38.0	23.0	41.0
47	33.15526800452058	35.0	33.0	38.0	23.0	41.0
48	33.1911037021804	35.0	33.0	38.0	23.0	41.0
49	33.26154258426496	35.0	33.0	38.0	24.0	40.0
50	33.025238085705396	35.0	32.0	38.0	23.0	40.0
51	32.72142653056396	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	4.0
12	3.0
13	13.0
14	21.0
15	61.0
16	175.0
17	575.0
18	1461.0
19	2933.0
20	5309.0
21	8373.0
22	12792.0
23	18479.0
24	25998.0
25	35452.0
26	44828.0
27	51019.0
28	56561.0
29	63556.0
30	75716.0
31	92159.0
32	118476.0
33	158395.0
34	295552.0
35	429576.0
36	114407.0
37	136630.0
38	193146.0
39	256158.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.353107534846135	24.097112397563546	30.456089043052888	14.093691024537433
2	31.055602771312152	26.5487172580971	30.848273025567487	11.547406945023267
3	28.34143482921343	26.214769412197196	33.64701985670271	11.796775901886669
4	26.523147954364706	27.174573738778168	32.338845141013344	13.963433165843778
5	22.38829126075776	32.47820239605305	32.47042241217717	12.663083931012018
6	21.318247747444435	40.67107138112458	28.971932002030986	9.038748869400004
7	85.4642148039899	5.11609009858286	8.034721476577245	1.384973620849993
8	87.55066088915662	3.892630762875191	7.348263016231503	1.2084453317366926
9	83.88305819791914	5.32601317228615	8.724592210552752	2.0663364192419476
10	64.27517848465932	17.049447939623686	12.528503859053991	6.146869716662996
11	61.410597520960465	15.267285668268768	16.81313661651686	6.508980194253914
12	53.43165534398449	21.68226909826802	17.735587921097316	7.150487636650185
13	17.51897679107968	54.902026799542114	18.921330258950622	8.65766615042758
14	9.562237141188055	54.49965149311995	27.303830663873757	8.634280701818241
15	8.856715445497345	22.1916533057197	61.14102790420883	7.810603344574122
16	9.561873165334212	15.719707654594195	58.64397155892678	16.074447621144813
17	10.692564155293937	15.538857152216522	31.777639871370933	41.990938821118604
18	16.157206630912103	21.604696744417975	45.90731900845698	16.33077761621294
19	36.44508423311198	17.963436805602317	28.78666829242548	16.804810668860227
20	35.950441047740895	20.68975244182301	28.60609077193819	14.753715738497908
21	17.299681339139962	35.02525992425662	30.648268293881387	17.026790442722028
22	18.49597897675468	25.014650028117135	21.665480712009565	34.82389028311862
23	12.715405460001783	35.075625083031994	26.58725320162261	25.621716255343618
24	10.677004187542199	20.725831548335083	56.23886461372153	12.358299650401193
25	9.899324278827343	24.317317789137867	50.863032246440774	14.920325685594019
26	9.120825424441342	44.88331844065465	29.869905930440577	16.125950204463436
27	9.934584439668273	51.79553838398361	27.471760023440044	10.798117152908077
28	8.412209933992978	34.76997635976829	45.3964334006082	11.421380305630525
29	9.494082662556167	26.673788460873503	43.49575149184603	20.336377384724297
30	14.745025814987434	44.117194765299274	29.096775719898744	12.041003699814553
31	35.71453919746964	29.33517990416516	25.216338148127253	9.733942750237949
32	39.417675031438414	21.391588881993567	29.535002647924337	9.655733438643681
33	38.23630040383121	26.48452101687574	20.35898938464422	14.920189194648827
34	19.512427045589796	26.307810739835517	24.45890439628235	29.720857818292334
35	19.72721829633822	23.591322451668557	36.46355600769445	20.217903244298775
36	39.67186666836522	26.491345564135276	22.93334510188594	10.903442665613564
37	16.394564384598727	46.672805726068134	26.564914183593057	10.367715705740082
38	16.429415072604083	46.436448905979574	20.524234422288426	16.609901599127912
39	20.429054736508782	41.359941181502016	25.177938695546935	13.033065386442264
40	29.30951960646931	23.386586033882512	24.387747116856268	22.91614724279191
41	13.696593367995968	23.27607386525978	26.936260548475214	36.09107221826904
42	15.396770078273006	20.752037809811696	26.27646331942339	37.57472879249191
43	16.68410717632992	20.783567218150747	29.244549916558537	33.2877756889608
44	11.800597648352008	31.55602407336297	36.20445069674078	20.43892758154424
45	11.374927887283958	54.15951605770473	21.5207548131257	12.944801241885614
46	17.882406681140772	44.328346257509274	22.660636193394932	15.12861086795502
47	17.553099527377352	30.041293060618358	24.91564859587215	27.489958816132138
48	17.95461039114665	26.75108783283317	41.08891566133503	14.205386114685151
49	22.147521233441374	27.254102462842617	39.054973093085	11.543403210631006
50	18.277002003687077	46.698966490563016	22.812687106337364	12.211344399412543
51	15.868573778815515	41.83147190015414	24.480469965622483	17.819484355407862
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2755.0
1	4112.5
2	5470.0
3	13161.0
4	20852.0
5	16208.5
6	11565.0
7	12374.5
8	13184.0
9	13913.0
10	14642.0
11	14411.0
12	14180.0
13	14077.5
14	13975.0
15	12565.5
16	11156.0
17	10815.5
18	10475.0
19	10519.5
20	10564.0
21	10366.0
22	10168.0
23	11396.0
24	12624.0
25	16461.5
26	23051.5
27	25804.0
28	33381.5
29	40959.0
30	48486.0
31	56013.0
32	61950.0
33	67887.0
34	68833.5
35	69780.0
36	74972.0
37	80164.0
38	107338.0
39	134512.0
40	190869.0
41	247226.0
42	307486.0
43	367746.0
44	341299.0
45	314852.0
46	257041.0
47	199230.0
48	170736.5
49	142243.0
50	116037.5
51	89832.0
52	72612.5
53	55393.0
54	42659.0
55	29925.0
56	26223.5
57	22522.0
58	21929.5
59	21337.0
60	20161.5
61	18986.0
62	16630.0
63	14274.0
64	11247.0
65	8220.0
66	6876.0
67	5532.0
68	4937.5
69	4343.0
70	3866.0
71	3389.0
72	2968.0
73	2547.0
74	2028.5
75	1168.5
76	827.0
77	689.5
78	552.0
79	404.0
80	256.0
81	169.0
82	82.0
83	68.5
84	55.0
85	36.5
86	18.0
87	14.5
88	11.0
89	6.0
90	1.0
91	4.5
92	8.0
93	4.5
94	1.0
95	0.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2197948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.390570716366533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.83730873894662	12.441323166675124
2	6.723550989080236	2.0695857392507104
3	2.341498790888138	1.0811100817035189
4	1.3024727061343937	0.801831931595947
5	0.8919535938555472	0.6863837430975537
6	0.6682398491629562	0.617075559242195
7	0.5435994227165463	0.585641374968652
8	0.4534531864954736	0.5583122666656264
9	0.4018947191166072	0.5566850185588567
>10	3.7538973660553996	11.84264176841017
>50	0.5625137969846125	6.243195760843536
>100	1.4365452444374858	52.31513660318134
>500	0.07268764661024767	7.517008414187763
>1k	0.010087265243871105	2.20521744184175
>5k	0.0	0.0
>10k+	2.966842718785619E-4	0.4788511297772943
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10487	0.4771268474049431	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3457	0.15728306584141208	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3355	0.15264237370492842	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010646293724874292	0.0
2	0.0	0.0	0.0	0.040947283557208815	0.0
3	0.0	0.0	0.0	0.05714420905317141	0.0
4	0.0	0.0	0.0	0.08735420492204547	0.0
5	0.0	0.0	0.0	0.16306118252115154	0.0
6	0.0	0.0	0.0	0.23394548005685303	0.0
7	0.0	0.0	0.0	0.28840536718794074	0.0
8	0.0	0.0	0.0	0.40069191809815335	0.0
9	0.0	0.0	0.0	0.44627989379184585	0.0
10	0.0	0.0	0.0	0.5214864045919194	0.0
11	0.0	0.0	0.0	0.5943270723420209	0.0
12	0.0	0.0	0.0	0.6524722149932574	0.0
13	0.0	0.0	0.0	0.6788149674150616	0.0
14	0.0	0.0	0.0	0.6881418486697592	0.0
15	0.0	0.0	0.0	0.7035198284945776	0.0
16	0.0	0.0	0.0	0.7390529712258889	0.0
17	0.0	0.0	0.0	0.7815926491436558	0.0
18	0.0	0.0	0.0	0.8468808179265388	0.0
19	0.0	0.0	0.0	0.8772728017223338	0.0
20	0.0	0.0	0.0	0.9086202221344636	0.0
21	0.0	0.0	0.0	0.9589853809098304	0.0
22	0.0	0.0	0.0	1.0103969702649926	0.0
23	0.0	0.0	0.0	1.0727733322171407	0.0
24	0.0	0.0	0.0	1.1178608411118007	0.0
25	0.0	0.0	0.0	1.1546678993315584	0.0
26	0.0	0.0	0.0	1.199709911244488	0.0
27	0.0	0.0	0.0	1.2394742732767108	0.0
28	0.0	0.0	0.0	1.2836973395184963	0.0
29	0.0	0.0	0.0	1.3320151341160027	0.0
30	0.0	0.0	0.0	1.408131584550681	0.0
31	0.0	0.0	0.0	1.4805627794652103	0.0
32	0.0	0.0	0.0	1.5448500146500281	0.0
33	0.0	0.0	0.0	1.6027676723926134	0.0
34	0.0	0.0	0.0	1.6691932657187523	0.0
35	0.0	0.0	0.0	1.7700600742146766	0.0
36	0.0	0.0	0.0	1.8484513737358663	0.0
37	0.0	0.0	0.0	1.9296179891426002	0.0
38	0.0	0.0	0.0	2.0053249667417066	0.0
39	0.0	0.0	0.0	2.0832612964455937	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAT	30	2.166409E-6	45.000004	24
ACCGGGT	75	0.0	45.000004	4
GTCGATG	30	2.166409E-6	45.000004	1
CGACACT	30	2.166409E-6	45.000004	34
ACTCGTT	35	1.2126111E-7	45.000004	38
TCGGTAT	30	2.166409E-6	45.000004	1
TATCCGT	35	1.2126111E-7	45.000004	15
ACGTTAT	45	3.8562575E-10	45.0	17
CGGCCTT	20	7.0346944E-4	45.0	44
CGCATGA	135	0.0	45.0	38
TACGGGT	95	0.0	45.0	4
ATGTACG	40	6.8193913E-9	45.0	1
ACGATTT	20	7.0346944E-4	45.0	31
TACGCTA	20	7.0346944E-4	45.0	16
GGACGTC	45	3.8562575E-10	45.0	8
ACCGTAA	20	7.0346944E-4	45.0	18
GCTACCT	45	3.8562575E-10	45.0	1
CGGTAGT	85	0.0	45.0	12
CTTAACG	25	3.8919727E-5	44.999996	1
CAAGTCG	25	3.8919727E-5	44.999996	1
>>END_MODULE