FastQCFastQC Report
Thu 26 May 2016
SRR1545070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545070_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2153206
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75010.34836425311837327No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG31690.1471758856328656No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG31460.14610771101325187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25210.11708122678461791No Hit
AGTGTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA22940.10653880771277806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC406.8193913E-945.00000425
AACGTAG650.045.0000041
CGGGTCA207.034659E-445.0000046
CTCACGA406.8193913E-945.00000424
CGAACAT650.045.00000443
GTCGACA600.045.0000048
CAAGTCG302.166389E-645.0000041
GGTCGAC600.045.0000047
ACGCATG406.8193913E-945.0000041
ACACCGA207.034659E-445.00000419
CTATACG207.034659E-445.0000041
TAAGTCG600.045.0000041
ATGTCGT650.045.00000411
TCGATGC207.034659E-445.00000436
TAGTTCG600.045.0000042
AGACGCG600.045.0000041
TACGCCT207.034659E-445.00000434
CTTGCCC207.034659E-445.00000441
ACGTGCA406.8193913E-945.00000433
CGAATCC406.8193913E-945.00000428