##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545070_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2153206
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.882236534730072	31.0	31.0	33.0	30.0	33.0
2	31.197490161182905	31.0	31.0	33.0	30.0	34.0
3	31.34713353018708	31.0	31.0	34.0	28.0	34.0
4	35.17554195929233	37.0	35.0	37.0	32.0	37.0
5	35.03622737443607	37.0	35.0	37.0	32.0	37.0
6	35.1187201781901	37.0	35.0	37.0	32.0	37.0
7	35.55484612247969	36.0	35.0	37.0	35.0	37.0
8	35.632807543727814	37.0	35.0	37.0	35.0	37.0
9	37.50985460750156	39.0	37.0	39.0	35.0	39.0
10	37.047185452762065	39.0	37.0	39.0	33.0	39.0
11	36.52396612307415	39.0	35.0	39.0	32.0	39.0
12	34.660032992663034	35.0	33.0	39.0	30.0	39.0
13	34.00997210670972	35.0	33.0	39.0	25.0	39.0
14	34.53965528611754	35.0	33.0	39.0	25.0	41.0
15	35.146470425960175	35.0	33.0	39.0	30.0	41.0
16	35.45539442115617	35.0	34.0	39.0	31.0	41.0
17	35.339589895253866	35.0	34.0	39.0	31.0	41.0
18	35.481161115099994	36.0	34.0	39.0	31.0	41.0
19	35.323288621711065	36.0	34.0	38.0	30.0	41.0
20	34.98675091932681	35.0	34.0	38.0	30.0	41.0
21	34.79339738046429	35.0	34.0	38.0	29.0	41.0
22	34.612557739482426	35.0	33.0	38.0	29.0	41.0
23	34.62933644063782	35.0	34.0	38.0	29.0	41.0
24	34.74611672083395	35.0	34.0	38.0	29.0	41.0
25	34.69087165835503	35.0	34.0	38.0	30.0	41.0
26	34.44749364436101	35.0	34.0	38.0	29.0	40.0
27	34.41116641881919	35.0	34.0	38.0	29.0	40.0
28	34.57167823236606	35.0	34.0	38.0	29.0	40.0
29	34.59103448532096	35.0	34.0	38.0	29.0	40.0
30	34.570702478072235	35.0	34.0	38.0	29.0	40.0
31	34.23929898021834	35.0	34.0	38.0	29.0	40.0
32	34.05124916055408	35.0	34.0	38.0	27.0	41.0
33	33.807244174500724	35.0	33.0	38.0	27.0	41.0
34	33.6537042902537	35.0	33.0	38.0	25.0	41.0
35	33.418144850051505	35.0	33.0	38.0	23.0	41.0
36	33.10925336451784	35.0	33.0	38.0	23.0	40.0
37	33.15828397282935	35.0	32.0	38.0	23.0	40.0
38	33.25788428975212	35.0	32.0	38.0	23.0	40.0
39	33.28779178583006	35.0	33.0	38.0	23.0	40.0
40	32.88054185247487	35.0	32.0	38.0	23.0	40.0
41	33.07596904337068	35.0	32.0	38.0	23.0	40.0
42	33.053974863529085	35.0	32.0	38.0	23.0	40.0
43	33.01031810240172	35.0	32.0	38.0	23.0	40.0
44	33.00625393018596	35.0	32.0	38.0	23.0	40.0
45	33.11418182932799	35.0	33.0	38.0	23.0	40.0
46	33.151967809861205	35.0	33.0	38.0	23.0	40.0
47	33.09742402724124	35.0	33.0	38.0	23.0	40.0
48	33.175257731958766	35.0	33.0	38.0	23.0	40.0
49	33.2806452332011	35.0	33.0	37.0	24.0	40.0
50	32.97473163273742	35.0	32.0	37.0	24.0	40.0
51	32.67596690702144	35.0	32.0	37.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	4.0
12	4.0
13	3.0
14	10.0
15	47.0
16	166.0
17	528.0
18	1372.0
19	2874.0
20	5055.0
21	8037.0
22	12171.0
23	17873.0
24	25016.0
25	34432.0
26	43736.0
27	50224.0
28	55842.0
29	64192.0
30	76470.0
31	94223.0
32	121221.0
33	163087.0
34	314235.0
35	468049.0
36	95724.0
37	111669.0
38	158463.0
39	228365.0
40	108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.726695912978133	25.192573306966448	31.394859572191418	13.685871207863995
2	30.34656228897746	26.131823894230276	31.517838980571295	12.003774836220964
3	27.406249100178986	25.835475100849614	34.018389322712274	12.739886476259121
4	25.99686235316082	28.113427140738047	33.367778094617975	12.521932411483155
5	21.965478453989075	33.670489493341556	31.714661764828815	12.64937028784055
6	19.601608020783893	41.291590307662155	29.3727121325131	9.734089539040854
7	87.41620634532879	4.7802207498957365	6.77194843410245	1.0316244706730335
8	90.461479301098	2.63286466784878	5.89595236126966	1.0097036697835693
9	87.64721071741394	4.190216820870832	6.788203265270484	1.3743691964447433
10	70.45099261287588	15.26992772637639	10.338258392369331	3.940821268378409
11	67.51374462081195	12.221125150124976	15.03047084208385	5.23465938697923
12	59.64844979997269	19.36725979771559	15.508409320798847	5.47588108151287
13	16.67462379354321	60.971082190928314	15.648990389214967	6.705303626313507
14	7.745287724444387	59.972153152090414	24.34472131324174	7.937837810223453
15	6.733029724048698	20.749849294493885	65.44320422662764	7.073916754829774
16	6.877976375692804	13.565074591098112	63.926767805774276	15.63018122743481
17	7.76576881171611	14.301789982008223	29.909028676308726	48.02341252996694
18	15.01050062093455	20.69462931089733	48.37581726968994	15.919052798478175
19	36.904736472032866	16.97009018180332	27.79000244286891	18.3351709032949
20	36.777716577048366	19.133979749266906	27.61867652235782	16.469627151326904
21	15.013890914292455	37.13411536100122	31.14430296032985	16.70769076437647
22	18.578900486065894	23.501234902745022	19.353791509033506	38.56607310215558
23	11.218759375554406	36.482110861663955	24.013354969287658	28.285774793493978
24	8.733070593338493	19.028648443298042	61.1174221138154	11.12085884954807
25	7.184170952523818	23.9601784501808	53.63151505243808	15.224135544857296
26	7.092958128483759	46.267751436694866	29.586300614061077	17.0529898207603
27	7.755319277393803	56.09421485914492	26.16558750068502	9.98487836277625
28	6.494594572001007	35.575973687608155	47.44576227262974	10.483669467761096
29	7.292706782351526	24.563093359390603	45.05579122480617	23.0884086334517
30	12.930485982298023	46.617648288180504	28.781036277996623	11.670829451524844
31	36.950993077299614	29.134648519463536	24.655792339423165	9.258566063813682
32	41.12727718574071	19.975840676646822	29.541344395287773	9.355537742324701
33	40.77584773588779	26.664099951421278	18.46321253052425	14.096839782166684
34	19.510255869619535	25.173764145186293	23.275432076633635	32.040547908560534
35	18.284084291052505	22.94504102254963	37.893169534173694	20.87770515222417
36	43.65625026123836	23.840171353785937	21.46236820815101	11.041210176824698
37	15.090706602155112	48.408605586274604	27.059928311550312	9.44075950001997
38	15.482308706180458	46.48138636061761	20.85866377857019	17.177641154631747
39	19.79759484229563	41.921348909486596	25.589005417967442	12.692050830250334
40	30.916688881602596	22.23461201575697	22.970770098169893	23.877929004470545
41	11.520356157283604	22.472768513556066	26.596758508010844	39.41011682114949
42	15.447430482731331	17.58168981509433	25.801339955396745	41.169539746777595
43	16.40432917240617	18.213259669534636	29.01886767917236	36.36354347888683
44	9.722060963976507	30.868249484721854	37.669410172552	21.74027937874964
45	9.283691388561985	58.44633537153435	19.41309842160945	12.856874818294209
46	17.83131757946058	46.26988778593409	21.640474715377906	14.258319919227421
47	16.6098366807449	29.43350520108155	24.004345148583088	29.952312969590462
48	17.532321570718267	25.602241494775697	43.00340979915531	13.862027135350727
49	22.65175742590351	25.451953970033518	40.34393365056572	11.55235495349725
50	18.32885474032675	48.35236387043321	21.844867606722254	11.47391378251779
51	15.80085695469918	43.28099587313058	21.994737150091538	18.923410022078706
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3404.0
1	3607.0
2	3810.0
3	8796.5
4	13783.0
5	11372.5
6	8962.0
7	9797.5
8	10633.0
9	11535.0
10	12437.0
11	12822.0
12	13207.0
13	12594.5
14	11982.0
15	10781.0
16	9580.0
17	8953.0
18	8326.0
19	7600.5
20	6875.0
21	6964.0
22	7053.0
23	7504.0
24	7955.0
25	9759.5
26	16567.5
27	21571.0
28	25947.5
29	30324.0
30	41221.5
31	52119.0
32	56644.0
33	61169.0
34	62944.5
35	64720.0
36	67294.0
37	69868.0
38	94440.0
39	119012.0
40	195898.0
41	272784.0
42	331887.0
43	390990.0
44	362662.5
45	334335.0
46	286978.5
47	239622.0
48	199110.5
49	158599.0
50	128311.0
51	98023.0
52	72717.0
53	47411.0
54	34162.0
55	20913.0
56	16363.0
57	11813.0
58	10168.0
59	8523.0
60	7787.0
61	7051.0
62	6387.5
63	5724.0
64	4622.5
65	3521.0
66	2706.5
67	1892.0
68	1549.5
69	1207.0
70	1050.5
71	894.0
72	771.5
73	649.0
74	536.5
75	333.5
76	243.0
77	190.5
78	138.0
79	101.0
80	64.0
81	39.0
82	14.0
83	11.0
84	8.0
85	6.5
86	5.0
87	3.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2153206.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.278421960467519
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.69473633436071	11.521934953706722
2	8.118453087638164	2.318373977031162
3	2.6378718322663794	1.129939412961929
4	1.316629605309089	0.7519757228098791
5	0.7813419083049965	0.557816473108783
6	0.5610121607472753	0.4806221013661938
7	0.44248202143920173	0.44225615084206904
8	0.3664376085481947	0.41857206376285955
9	0.2929605047654334	0.3764712334313172
>10	2.908682453047415	8.959315924037483
>50	0.44407175408610766	4.509497536368036
>100	1.2630430879391865	50.39814558994866
>500	0.1513955031784497	13.749273022070064
>1k	0.020555854957418816	4.036348332765645
>5k	3.262834120225209E-4	0.3494575057892531
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7501	0.34836425311837327	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3169	0.1471758856328656	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3146	0.14610771101325187	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2521	0.11708122678461791	No Hit
AGTGTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2294	0.10653880771277806	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02289609075954646	0.0
2	0.0	0.0	0.0	0.09683235138672287	0.0
3	0.0	0.0	0.0	0.13667990893579157	0.0
4	0.0	0.0	0.0	0.19645124525939459	0.0
5	0.0	0.0	0.0	0.3488751192407972	0.0
6	0.0	0.0	0.0	0.47097212250012305	0.0
7	0.0	0.0	0.0	0.5624171584140115	0.0
8	0.0	0.0	0.0	0.742381360631542	0.0
9	0.0	0.0	0.0	0.7991803849701329	0.0
10	0.0	0.0	0.0	0.90395438244181	0.0
11	0.0	0.0	0.0	1.0422133321196392	0.0
12	0.0	0.0	0.0	1.1455476159735762	0.0
13	0.0	0.0	0.0	1.1925008568618145	0.0
14	0.0	0.0	0.0	1.2090807846532101	0.0
15	0.0	0.0	0.0	1.2404293876201349	0.0
16	0.0	0.0	0.0	1.313947666874419	0.0
17	0.0	0.0	0.0	1.4058571265359654	0.0
18	0.0	0.0	0.0	1.5417010727259723	0.0
19	0.0	0.0	0.0	1.5962708630758042	0.0
20	0.0	0.0	0.0	1.6514908466723575	0.0
21	0.0	0.0	0.0	1.7472550234394666	0.0
22	0.0	0.0	0.0	1.8407899662178167	0.0
23	0.0	0.0	0.0	1.9678098612023187	0.0
24	0.0	0.0	0.0	2.0523814256508666	0.0
25	0.0	0.0	0.0	2.1211625826790375	0.0
26	0.0	0.0	0.0	2.204805299632269	0.0
27	0.0	0.0	0.0	2.273818668534269	0.0
28	0.0	0.0	0.0	2.3487766614062937	0.0
29	0.0	0.0	0.0	2.4341377462258604	0.0
30	0.0	0.0	0.0	2.5693779415439115	0.0
31	0.0	0.0	0.0	2.700113226509679	0.0
32	0.0	0.0	0.0	2.8002894288795406	0.0
33	0.0	0.0	0.0	2.897771973512985	0.0
34	0.0	0.0	0.0	3.0041250117266998	0.0
35	0.0	0.0	0.0	3.1807918053358573	0.0
36	0.0	0.0	0.0	3.3031210204690122	0.0
37	0.0	0.0	0.0	3.4282832204628817	0.0
38	0.0	0.0	0.0	3.542206365763424	0.0
39	0.0	0.0	0.0	3.649859790470582	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	40	6.8193913E-9	45.000004	25
AACGTAG	65	0.0	45.000004	1
CGGGTCA	20	7.034659E-4	45.000004	6
CTCACGA	40	6.8193913E-9	45.000004	24
CGAACAT	65	0.0	45.000004	43
GTCGACA	60	0.0	45.000004	8
CAAGTCG	30	2.166389E-6	45.000004	1
GGTCGAC	60	0.0	45.000004	7
ACGCATG	40	6.8193913E-9	45.000004	1
ACACCGA	20	7.034659E-4	45.000004	19
CTATACG	20	7.034659E-4	45.000004	1
TAAGTCG	60	0.0	45.000004	1
ATGTCGT	65	0.0	45.000004	11
TCGATGC	20	7.034659E-4	45.000004	36
TAGTTCG	60	0.0	45.000004	2
AGACGCG	60	0.0	45.000004	1
TACGCCT	20	7.034659E-4	45.000004	34
CTTGCCC	20	7.034659E-4	45.000004	41
ACGTGCA	40	6.8193913E-9	45.000004	33
CGAATCC	40	6.8193913E-9	45.000004	28
>>END_MODULE