FastQCFastQC Report
Thu 26 May 2016
SRR1545069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545069_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3592801
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG113600.3161878434124239No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110550.3076986451517911No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG87530.24362607336170303No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG85600.23825422003612226No Hit
ATTGATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA39700.11049874457282771No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39170.109023572416062No Hit
GGATATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA38810.10802156868693813No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37900.10548872592720832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC453.8562575E-1045.00000425
TCGCCTC207.0352905E-445.00000419
GTGCACC253.892467E-545.00000418
CAGGTAC253.892467E-545.00000410
ACACGGC253.892467E-545.00000415
ATTAGCC502.1827873E-1145.00000429
CACGGGT900.045.0000044
CGTGCGC253.892467E-545.00000418
GACACGT207.0352905E-445.0000048
ATAATCG1600.045.0000041
GGCGTGT800.045.0000047
GACGTAA800.045.00000411
CGCATCA207.0352905E-445.00000433
CGCATAT850.045.00000435
CCGGATG406.8212103E-945.0000041
ATAGCCG453.8562575E-1045.0000042
CGACACT207.0352905E-445.0000048
TCCAGCG351.2129021E-745.0000046
CGCTCAC453.8562575E-1045.0000045
GTGCTAT253.892467E-545.00000423