FastQCFastQC Report
Thu 26 May 2016
SRR1545068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545068_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences286937
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15270.5321725674973949No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10480.3652369683937589No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6920.2411679218783217No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6180.2153782886138769No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA5130.1787848900629755No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA5000.17425427881381628No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3990.13905491449342539No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3120.10873466997982137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGAGG351.2076634E-745.0000042
GCGTTAG253.883464E-545.0000041
AGTATCG253.883464E-545.0000041
TGTTACG253.883464E-545.0000041
GCGATTC351.2076634E-745.0000049
TATCGGA253.883464E-545.00000435
GTACGAG351.2076634E-745.0000041
AGGTCAA207.024429E-445.033
GCCCCGT207.024429E-445.06
AGCGTGG207.024429E-445.02
AAATCGA207.024429E-445.044
GTCTGAT207.024429E-445.033
GCGAAAG207.024429E-445.01
AGCCCCG207.024429E-445.05
GGCACCG453.8198777E-1045.08
GTTTACG207.024429E-445.01
CAGCACA950.045.045
CGGCAGG207.024429E-445.02
ATAGCGG453.8198777E-1045.02
ATTGTGT207.024429E-445.021