##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545067_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3167247
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.05000620412617	31.0	31.0	33.0	30.0	33.0
2	31.37628830337514	31.0	31.0	33.0	30.0	34.0
3	31.54994605725414	31.0	31.0	34.0	30.0	34.0
4	35.359579470751726	37.0	35.0	37.0	33.0	37.0
5	35.21544325403102	37.0	35.0	37.0	33.0	37.0
6	35.28536454529754	37.0	35.0	37.0	32.0	37.0
7	35.682325375949524	37.0	35.0	37.0	35.0	37.0
8	35.772908143886475	37.0	35.0	37.0	35.0	37.0
9	37.688424679224575	39.0	37.0	39.0	35.0	39.0
10	37.29030100904666	39.0	37.0	39.0	34.0	39.0
11	36.73183114547113	39.0	35.0	39.0	33.0	39.0
12	34.56606384030042	35.0	34.0	38.0	30.0	39.0
13	33.82607671583555	35.0	33.0	37.0	25.0	39.0
14	34.27843676227336	35.0	33.0	38.0	25.0	41.0
15	34.95034741527895	35.0	33.0	39.0	30.0	41.0
16	35.30276230429771	35.0	34.0	38.0	32.0	41.0
17	35.2250503355122	35.0	34.0	38.0	31.0	41.0
18	35.40333734628212	36.0	35.0	38.0	31.0	41.0
19	35.24432669760205	36.0	34.0	37.0	31.0	41.0
20	34.879860964427465	35.0	34.0	37.0	31.0	41.0
21	34.68248860919278	35.0	34.0	37.0	30.0	41.0
22	34.502884681870405	35.0	34.0	37.0	29.0	41.0
23	34.57032558559531	35.0	34.0	37.0	30.0	41.0
24	34.705112357830004	35.0	34.0	37.0	31.0	41.0
25	34.660722387612964	35.0	34.0	37.0	30.0	40.0
26	34.42935962998781	35.0	34.0	36.0	30.0	40.0
27	34.37436265627531	35.0	34.0	37.0	29.0	40.0
28	34.59998478173632	35.0	34.0	37.0	30.0	40.0
29	34.65338289056711	35.0	34.0	36.0	30.0	40.0
30	34.61474744470513	35.0	34.0	36.0	30.0	40.0
31	34.245180751611734	35.0	34.0	36.0	29.0	40.0
32	34.046226265270754	35.0	34.0	36.0	29.0	40.0
33	33.80876925607633	35.0	33.0	36.0	27.0	40.0
34	33.6871825910641	35.0	34.0	36.0	27.0	40.0
35	33.43898123512312	35.0	33.0	36.0	26.0	40.0
36	33.09254914441469	35.0	33.0	36.0	23.0	40.0
37	33.107507560982775	35.0	33.0	36.0	23.0	40.0
38	33.2476358806244	35.0	33.0	36.0	24.0	40.0
39	33.27685147385095	35.0	33.0	36.0	24.0	40.0
40	32.83170242169304	35.0	32.0	36.0	23.0	40.0
41	33.10204540410015	35.0	33.0	36.0	23.0	40.0
42	33.09776092612922	35.0	33.0	36.0	23.0	40.0
43	33.07398696723053	35.0	33.0	36.0	23.0	40.0
44	33.037380096973806	35.0	33.0	35.0	23.0	40.0
45	33.121146219413895	35.0	33.0	35.0	23.0	40.0
46	33.19403223051439	35.0	33.0	35.0	23.0	40.0
47	33.17278033573005	35.0	33.0	35.0	24.0	40.0
48	33.24211184034589	35.0	33.0	36.0	23.0	40.0
49	33.35824297883935	35.0	33.0	36.0	24.0	40.0
50	33.008853745855625	35.0	33.0	36.0	24.0	40.0
51	32.696268399654336	35.0	32.0	35.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	3.0
12	7.0
13	10.0
14	18.0
15	73.0
16	189.0
17	551.0
18	1413.0
19	3119.0
20	5815.0
21	9637.0
22	15301.0
23	22709.0
24	32875.0
25	46257.0
26	58980.0
27	68316.0
28	75883.0
29	87324.0
30	107095.0
31	135205.0
32	179127.0
33	248934.0
34	515199.0
35	804606.0
36	115147.0
37	136397.0
38	193074.0
39	303719.0
40	260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.818482739110653	25.30516249600994	32.18107081639038	12.695283948489019
2	28.571974336071676	26.447637332989814	32.93601667315495	12.044371657783557
3	27.257804648642814	25.40136591809859	35.87317313742818	11.467656295830416
4	25.834612835689796	28.616460920161895	32.976335599970575	12.572590644177737
5	22.074280913361036	33.97688907748591	31.68691927089993	12.261910738253126
6	20.35720611622649	41.708619504572894	28.92995083743074	9.004223541769871
7	87.9954736716145	4.968226349255363	6.010330106871993	1.0259698722581472
8	91.09538978172526	2.651324636190357	5.410471617780362	0.8428139643040156
9	89.18268767797396	3.8866877133359035	5.978820092023136	0.9518045166669983
10	77.19243241843785	10.543446722027047	8.45778684137991	3.806334018155199
11	76.07988893824826	9.248662955557304	10.82555291709172	3.8458951891027127
12	64.65889777462888	19.5698346229391	11.85101761877113	3.920249983660889
13	15.683873092310135	67.15617695746495	12.665289445376379	4.494660504848532
14	6.161249817270329	65.89457658338614	23.285995692789353	4.658177906554178
15	5.394779756678276	19.05860199725503	70.38206998064881	5.164548265417885
16	5.459252151789866	10.962738302380584	68.4547810764364	15.123228469393135
17	6.020970262186688	11.092914445889443	29.96179331766673	52.92432197425714
18	14.451035868058284	18.483275854393423	51.01730303951665	16.04838523803164
19	40.67680859749808	13.055975741708808	28.36561215465671	17.9016035061364
20	39.278370142903285	17.742064322738326	26.459666707396046	16.519898826962343
21	14.409769746407527	37.83392959248205	31.55709043216396	16.199210228946466
22	18.23703677041923	21.970531505752472	17.75583022100897	42.03660150281932
23	9.909899669965746	37.29724899889399	22.850949105011388	29.941902226128875
24	7.256712217266288	17.087205386886467	65.40490842678199	10.251173969065249
25	5.836772439913906	21.043764505894234	58.16420380222951	14.955259251962353
26	5.718278366038392	49.46611363117559	28.456195554056883	16.35941244872913
27	6.610725339703534	60.808803355090404	23.80837364436686	8.772097660839208
28	4.731964384211272	37.191952506388034	49.62966260604241	8.44642050335828
29	5.719509719324069	24.43585864948329	47.190414893439005	22.65421673775364
30	12.245650560249958	50.065309083882624	27.156991545023168	10.532048810844245
31	41.04173119431481	28.958145670356622	22.970611385850233	7.029511749478333
32	45.08413773854707	18.49931502026839	29.622129249786962	6.794417991397577
33	45.10289219628277	25.82084693741915	16.19387436470853	12.882386501589552
34	19.573844414407844	25.769098526259555	20.478320762479214	34.17873629685339
35	18.133508374938867	22.070934158276888	38.70828514479609	21.08727232198815
36	48.993715993732096	22.54582607545291	18.896757973091457	9.563699957723538
37	14.996872678385992	51.95666773068219	24.493069217525505	8.553390373406305
38	15.358069642184521	50.2770387026967	17.252711897745897	17.112179757372886
39	20.727780308892864	44.072628374105335	23.265631003833928	11.933960313167871
40	33.22623717064062	20.406744406104103	21.683910348640318	24.683108074614957
41	11.183024247872048	20.46667026600704	24.94885937219295	43.40144611392796
42	15.113756521041774	16.216543894429453	24.97222351145964	43.69747607306913
43	16.289130591962042	16.290298798925377	29.16782303369456	38.25274757541802
44	8.445473308523145	30.908861860158048	39.6288322319036	21.016832599415203
45	8.567093125354607	62.49891467258474	17.664899516835913	11.26909268522474
46	18.960851490268993	47.766467219007545	19.651167086116114	13.621514204607346
47	17.639198963642556	28.33116583581893	21.690161834552217	32.3394733659863
48	18.513838674407143	25.729411062667356	43.76677916183992	11.98997110108558
49	25.271063481945045	23.74519574886329	41.69318022876018	9.290560540431485
50	19.808338282426348	51.409094396490076	18.667757835116745	10.114809485966834
51	15.85023207852119	45.53351854149676	20.615979745185644	18.0002696347964
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5870.0
1	5760.0
2	5650.0
3	11654.5
4	17659.0
5	14508.5
6	11358.0
7	12698.0
8	14038.0
9	15792.0
10	17546.0
11	17776.0
12	18006.0
13	17177.0
14	16348.0
15	15230.5
16	14113.0
17	13014.0
18	11915.0
19	10639.0
20	9363.0
21	10232.5
22	11102.0
23	10959.5
24	10817.0
25	12214.0
26	15652.5
27	17694.0
28	30005.5
29	42317.0
30	43971.0
31	45625.0
32	57303.5
33	68982.0
34	73428.0
35	77874.0
36	81328.0
37	84782.0
38	130191.5
39	175601.0
40	289764.0
41	403927.0
42	522001.0
43	640075.0
44	605525.0
45	570975.0
46	475757.0
47	380539.0
48	298588.5
49	216638.0
50	173881.5
51	131125.0
52	98268.5
53	65412.0
54	45345.0
55	25278.0
56	18371.0
57	11464.0
58	9970.0
59	8476.0
60	7732.5
61	6989.0
62	6175.5
63	5362.0
64	4631.0
65	3900.0
66	3008.0
67	2116.0
68	1894.0
69	1672.0
70	1295.0
71	918.0
72	881.5
73	845.0
74	688.5
75	453.5
76	375.0
77	282.0
78	189.0
79	137.5
80	86.0
81	73.0
82	60.0
83	36.5
84	13.0
85	9.0
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3167247.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.780290369165455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.13302908619771	8.638573217099925
2	8.862191086433414	1.9107398643756415
3	2.9714735724745167	0.9610004380673011
4	1.4426299411690429	0.6220787864421744
5	0.8349367556930132	0.450043033312982
6	0.5662855388477755	0.3662833524383007
7	0.3858845632110099	0.2911963348275288
8	0.3014276799602864	0.259958233222061
9	0.2567941839728532	0.24914842815062263
>10	2.3984844500851343	5.7133308528478315
>50	0.5233908094072607	3.937377389632651
>100	0.713046080077668	20.250094486629227
>500	0.5095706625872222	40.00363471359037
>1k	0.10056155026245239	16.028606670551067
>5k	0.0	0.0
>10k+	2.940396206504456E-4	0.3179341988123015
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10030	0.316678806547137	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4792	0.15129858833239088	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4442	0.14024798192247084	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4201	0.13263885008021162	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3985	0.1258190472672324	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3529	0.11142168577316515	No Hit
TTTGTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	3478	0.10981145455343394	No Hit
TTGTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	3301	0.10422300502613152	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018217713995782456	0.0
2	0.0	0.0	0.0	0.06270429808600339	0.0
3	0.0	0.0	0.0	0.08543697412926747	0.0
4	0.0	0.0	0.0	0.13737482425589162	0.0
5	0.0	0.0	0.0	0.23430442905147594	0.0
6	0.0	0.0	0.0	0.32798199824642665	0.0
7	0.0	0.0	0.0	0.3969693554054988	0.0
8	0.0	0.0	0.0	0.5390485806758993	0.0
9	0.0	0.0	0.0	0.5886184437146834	0.0
10	0.0	0.0	0.0	0.6666672981298901	0.0
11	0.0	0.0	0.0	0.7670383774931352	0.0
12	0.0	0.0	0.0	0.8608738124939419	0.0
13	0.0	0.0	0.0	0.8961726066833436	0.0
14	0.0	0.0	0.0	0.9098122123093021	0.0
15	0.0	0.0	0.0	0.9290718406237342	0.0
16	0.0	0.0	0.0	0.9799993495928798	0.0
17	0.0	0.0	0.0	1.0370836249904096	0.0
18	0.0	0.0	0.0	1.1311084989582436	0.0
19	0.0	0.0	0.0	1.1667545979205285	0.0
20	0.0	0.0	0.0	1.2033794648791205	0.0
21	0.0	0.0	0.0	1.2696199570162985	0.0
22	0.0	0.0	0.0	1.3280303051830185	0.0
23	0.0	0.0	0.0	1.413151547700574	0.0
24	0.0	0.0	0.0	1.4662260316293614	0.0
25	0.0	0.0	0.0	1.5099864330126447	0.0
26	0.0	0.0	0.0	1.5645764286776498	0.0
27	0.0	0.0	0.0	1.6044217580757043	0.0
28	0.0	0.0	0.0	1.6523182435724149	0.0
29	0.0	0.0	0.0	1.704729691116607	0.0
30	0.0	0.0	0.0	1.8009015400440824	0.0
31	0.0	0.0	0.0	1.8930951706639867	0.0
32	0.0	0.0	0.0	1.9629350031746813	0.0
33	0.0	0.0	0.0	2.0253235696489726	0.0
34	0.0	0.0	0.0	2.1000888153023745	0.0
35	0.0	0.0	0.0	2.244346588693588	0.0
36	0.0	0.0	0.0	2.3316779524931275	0.0
37	0.0	0.0	0.0	2.4199249379666314	0.0
38	0.0	0.0	0.0	2.495763671099854	0.0
39	0.0	0.0	0.0	2.5709393678484815	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAG	60	0.0	45.000004	1
GTACCTA	30	2.1667147E-6	45.000004	17
CGTATAA	35	1.2128294E-7	45.000004	28
GATCGGT	95	0.0	45.000004	9
TAGACGC	65	0.0	45.000004	44
ATGTGCG	120	0.0	45.000004	1
ATTACGA	35	1.2128294E-7	45.000004	12
TCGATTG	70	0.0	45.000004	1
TGCGGGT	95	0.0	45.000004	4
TAGCCGT	30	2.1667147E-6	45.000004	44
ACGGGCC	70	0.0	45.000004	5
TACGCGT	35	1.2128294E-7	45.000004	24
TGTCTGC	35	1.2128294E-7	45.000004	39
GTATGCG	155	0.0	45.000004	1
CTACGCT	35	1.2128294E-7	45.000004	41
CTACGAT	35	1.2128294E-7	45.000004	14
TGACACG	130	0.0	45.000004	1
ACTACGA	35	1.2128294E-7	45.000004	13
ATCCATG	30	2.1667147E-6	45.000004	1
TATTACG	95	0.0	45.000004	1
>>END_MODULE