FastQCFastQC Report
Thu 26 May 2016
SRR1545066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545066_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3299742
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165300.5009482559545564No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG67630.204955417726598No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG56760.1720134483241417No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42080.12752512166102684No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG39280.11903960976343No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT33160.10049270518725405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA650.045.00000414
TCTAGCG900.045.0000041
CGTAGAT302.166742E-645.00000417
ATCGATA453.8562575E-1045.00000415
ATAGCCG551.8189894E-1245.01
ACCGAAC406.8212103E-945.011
CCGTACT207.035208E-445.043
TACGCGA351.2128476E-745.021
CGGATTA207.035208E-445.038
GTTCACG253.8923987E-545.044
CGTCGGT207.035208E-445.039
TTACGAG1400.045.01
ACCAACG207.035208E-445.038
TGGTCGA800.045.010
TATTGCG3000.044.251
GTTTACG850.042.352941
CTACGGG4250.042.352943
GTATGCG850.042.352941
CGAGTAC800.042.187540
ATGCTCG1600.042.18751