##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545066_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3299742
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.918416045860557	31.0	31.0	33.0	30.0	33.0
2	31.23323187085536	31.0	31.0	33.0	30.0	34.0
3	31.368333342424954	31.0	31.0	34.0	30.0	34.0
4	35.23608967004087	37.0	35.0	37.0	33.0	37.0
5	35.11423196116545	37.0	35.0	37.0	32.0	37.0
6	35.18413318374588	37.0	35.0	37.0	32.0	37.0
7	35.55793604469683	36.0	35.0	37.0	35.0	37.0
8	35.627112968226	37.0	35.0	37.0	35.0	37.0
9	37.4858716226905	39.0	37.0	39.0	35.0	39.0
10	37.03826450674023	39.0	37.0	39.0	33.0	39.0
11	36.559005219195924	39.0	35.0	39.0	32.0	39.0
12	35.044077385444076	35.0	35.0	39.0	31.0	39.0
13	34.49873111291731	35.0	33.0	39.0	27.0	39.0
14	35.11032832263856	35.0	33.0	40.0	27.0	41.0
15	35.6113853749778	35.0	34.0	40.0	30.0	41.0
16	35.83478738640778	35.0	34.0	40.0	31.0	41.0
17	35.68480111475382	35.0	34.0	40.0	31.0	41.0
18	35.79043058517908	36.0	35.0	39.0	31.0	41.0
19	35.64402368427592	36.0	34.0	39.0	31.0	41.0
20	35.33744668522569	35.0	34.0	39.0	30.0	41.0
21	35.18182057869979	35.0	34.0	39.0	30.0	41.0
22	35.014192624756724	35.0	34.0	39.0	30.0	41.0
23	34.99084443571649	35.0	34.0	39.0	29.0	41.0
24	35.091038026609354	35.0	34.0	39.0	30.0	41.0
25	34.98985647968841	35.0	34.0	39.0	30.0	41.0
26	34.73503019326966	35.0	34.0	39.0	29.0	41.0
27	34.723914778791794	35.0	34.0	39.0	29.0	41.0
28	34.83089496087876	35.0	34.0	39.0	29.0	41.0
29	34.82900147950961	36.0	34.0	39.0	29.0	41.0
30	34.784618918691216	35.0	34.0	39.0	29.0	41.0
31	34.493784059481015	35.0	34.0	39.0	29.0	41.0
32	34.324120188790516	35.0	34.0	39.0	27.0	41.0
33	34.07769880190633	35.0	33.0	39.0	27.0	41.0
34	33.881940163806746	35.0	33.0	39.0	25.0	41.0
35	33.66406373589208	35.0	33.0	39.0	24.0	41.0
36	33.36210982555606	35.0	33.0	38.0	23.0	41.0
37	33.4449705461821	35.0	33.0	39.0	23.0	41.0
38	33.48497124926737	35.0	33.0	38.0	23.0	41.0
39	33.47941414813643	35.0	33.0	38.0	23.0	41.0
40	33.157390183838615	35.0	32.0	38.0	23.0	41.0
41	33.25320100783637	35.0	32.0	38.0	23.0	41.0
42	33.20807263113298	35.0	32.0	38.0	23.0	41.0
43	33.171913743559344	35.0	33.0	38.0	23.0	41.0
44	33.16324639926394	35.0	33.0	38.0	23.0	41.0
45	33.23679911944631	35.0	33.0	38.0	23.0	41.0
46	33.24300233169745	35.0	33.0	38.0	23.0	41.0
47	33.17058970064932	35.0	33.0	38.0	23.0	41.0
48	33.222918943359815	35.0	33.0	38.0	23.0	41.0
49	33.27442418225425	35.0	33.0	38.0	24.0	40.0
50	33.04530475412926	35.0	32.0	38.0	23.0	40.0
51	32.746353805843	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	1.0
11	5.0
12	8.0
13	9.0
14	29.0
15	91.0
16	256.0
17	768.0
18	1887.0
19	3994.0
20	7161.0
21	11778.0
22	18137.0
23	26834.0
24	37641.0
25	51460.0
26	66345.0
27	76428.0
28	84082.0
29	95074.0
30	112321.0
31	137911.0
32	175753.0
33	235220.0
34	439745.0
35	649917.0
36	174309.0
37	206533.0
38	288574.0
39	397286.0
40	179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.50694327011021	24.71444737194605	30.906961817014782	14.871647540928956
2	30.743403575188605	26.2489309770279	31.309326607959047	11.698338839824448
3	27.113968304188628	26.375728769097705	34.69968258124423	11.810620345469433
4	25.46611219907496	28.401099237455536	33.07370697466651	13.059081588803004
5	22.783417612649718	32.44962787999789	31.96189277828388	12.805061729068514
6	20.920241643134524	40.55277655040909	29.72120244552453	8.805779360931854
7	85.17571979869942	5.269411972208736	8.132241854060105	1.4226263750317447
8	87.72207039217005	3.6252834312500792	7.381516494319859	1.271129682260007
9	84.21282633611962	5.333416976236324	8.612218773467744	1.8415379141763204
10	62.84754989935577	18.697492106958666	12.507614231658112	5.947343762027455
11	60.1694314282753	14.729121246448965	18.11135537263216	6.990091952643571
12	53.437299037318674	21.019097856741528	18.06771559715881	7.47588750878099
13	17.01730014043522	55.005209498197125	18.24163828565991	9.735852075707738
14	9.265330440985991	54.117958313104474	26.379819998048333	10.236891247861196
15	8.65076724180254	22.343110461363345	59.864710634952665	9.141411661881444
16	8.997339792020103	15.686408210096426	58.336015361200964	16.980236636682505
17	9.5596867876337	16.827224673929052	31.15961793376573	42.453470604671516
18	16.070377623462683	21.493953163610975	46.42378101075781	16.011888202168535
19	36.09827677436599	18.55451123148416	27.503241162490887	17.843970831658957
20	35.16053679348264	20.08338833763367	29.341021207112554	15.415053661771132
21	15.883666056315917	36.41808965670649	30.58457297570537	17.113671311272213
22	18.777831721389127	24.724690597022434	21.307999231455067	35.18947845013338
23	12.404272818905236	35.47941020843447	26.405306839140756	25.71101013351953
24	10.70259432404109	19.76257537710524	57.275053625404645	12.259776673449014
25	9.624510037451413	25.043473095775365	50.55464942410649	14.77736744266673
26	8.67501156150996	45.00891281803244	28.856316645361975	17.459758975095628
27	9.165140789795082	51.59012431881038	27.222825299674945	12.021909591719595
28	8.132726740454252	34.33710878001977	45.82464325998821	11.70552121953777
29	9.30054531536102	25.686462759815765	43.269958681618135	21.743033243205076
30	14.095889921090802	44.59775946119424	28.62290445737879	12.683446160336173
31	34.83375366922626	29.350749240395157	25.177180518961784	10.638316571416796
32	39.09017735325974	21.4400398576616	28.544625610123457	10.925157178955203
33	37.3124626107132	26.470311921356277	20.575972303289166	15.641253164641356
34	18.07850431942861	26.050006333828524	24.993257048581373	30.878232298161496
35	19.30329704564781	23.162568467474124	36.94043352480285	20.593700962075218
36	38.98298715475331	26.450704327792902	22.604979419603108	11.96132909785068
37	15.40632570667646	46.330258547486444	27.624977952821766	10.638437793015333
38	15.350230411953419	45.33560502608992	22.44854294669098	16.865621615265677
39	19.387061170236947	41.149489869207954	26.116890350821365	13.34655860973373
40	29.37438745210989	23.840863922088452	24.22337867627227	22.561369949529386
41	13.253339200458703	22.61037378073801	27.461540932594126	36.67474608620916
42	14.73000010303836	20.10117760721899	26.143316659302457	39.0255056304402
43	15.418660004327611	21.65566277605946	29.304109230358012	33.62156798925492
44	10.783994627458753	31.618229546431202	36.51385471955081	21.083921106559238
45	10.723414133589838	54.26209079376508	20.90490711091958	14.109587961725492
46	17.45397064376548	44.827716833619114	22.052451373471015	15.665861149144389
47	16.581750936891428	29.068060472606643	25.515934276073704	28.83425431442822
48	17.22343746874756	26.197502713848536	42.09450314600354	14.48455667140037
49	21.199445289965094	27.199278004159112	39.39198882821748	12.209287877658314
50	17.60567947433466	46.518424773815646	23.402284178581233	12.473611573268457
51	15.067450727966007	41.375446928881104	24.323507716663908	19.23359462648898
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5777.0
1	7570.5
2	9364.0
3	21218.5
4	33073.0
5	26095.0
6	19117.0
7	19310.5
8	19504.0
9	20682.5
10	21861.0
11	21675.0
12	21489.0
13	20656.5
14	19824.0
15	19646.0
16	19468.0
17	17315.0
18	15162.0
19	14501.0
20	13840.0
21	14890.5
22	15941.0
23	18200.0
24	20459.0
25	22052.5
26	31810.5
27	39975.0
28	52119.0
29	64263.0
30	69225.0
31	74187.0
32	89171.0
33	104155.0
34	109812.5
35	115470.0
36	120010.5
37	124551.0
38	162791.0
39	201031.0
40	288901.5
41	376772.0
42	455559.5
43	534347.0
44	496291.5
45	458236.0
46	393536.0
47	328836.0
48	266700.5
49	204565.0
50	173138.0
51	141711.0
52	108811.5
53	75912.0
54	60120.5
55	44329.0
56	38158.5
57	31988.0
58	31663.5
59	31339.0
60	28886.0
61	26433.0
62	23468.0
63	20503.0
64	16745.5
65	12988.0
66	10817.5
67	8647.0
68	7564.5
69	6482.0
70	5928.0
71	5374.0
72	4746.0
73	4118.0
74	3181.5
75	1821.5
76	1398.0
77	1023.5
78	649.0
79	511.5
80	374.0
81	270.5
82	167.0
83	125.5
84	84.0
85	71.0
86	58.0
87	35.5
88	13.0
89	9.0
90	5.0
91	5.5
92	6.0
93	4.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3299742.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.660260949921305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.60708049398009	11.817208344530597
2	7.75465263774657	2.273704624907206
3	2.6216880077140514	1.153038909671019
4	1.3037802349470826	0.7645503386269576
5	0.8654469615281025	0.6343839147159245
6	0.606209895922964	0.5332317158793174
7	0.4740066716958734	0.4864343049345627
8	0.39659817667150904	0.46513862098138514
9	0.31966256379958086	0.42177029411004513
>10	3.325338023795795	10.497536719588929
>50	0.4880496016380515	4.984696321347594
>100	0.9909026615302484	39.23830843285795
>500	0.2162799662704595	19.840587264428496
>1k	0.02968141571657938	6.007907225798854
>5k	4.151246953367745E-4	0.3785085924346859
>10k+	2.0756234766838725E-4	0.5029943751865389
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16530	0.5009482559545564	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	6763	0.204955417726598	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5676	0.1720134483241417	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4208	0.12752512166102684	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3928	0.11903960976343	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3316	0.10049270518725405	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023365463118025592	0.0
2	0.0	0.0	0.0	0.08661283215475635	0.0
3	0.0	0.0	0.0	0.12464610869577077	0.0
4	0.0	0.0	0.0	0.18483263236943978	0.0
5	0.0	0.0	0.0	0.32050990653208644	0.0
6	0.0	0.0	0.0	0.4685214783458828	0.0
7	0.0	0.0	0.0	0.5772269468340252	0.0
8	0.0	0.0	0.0	0.787243366299547	0.0
9	0.0	0.0	0.0	0.8688255021150139	0.0
10	0.0	0.0	0.0	0.9875014470828325	0.0
11	0.0	0.0	0.0	1.131330873747099	0.0
12	0.0	0.0	0.0	1.2418243608136637	0.0
13	0.0	0.0	0.0	1.2907069704237484	0.0
14	0.0	0.0	0.0	1.3111328097772492	0.0
15	0.0	0.0	0.0	1.3421049282034778	0.0
16	0.0	0.0	0.0	1.411595209564869	0.0
17	0.0	0.0	0.0	1.4920560455938676	0.0
18	0.0	0.0	0.0	1.6204903292439228	0.0
19	0.0	0.0	0.0	1.6761916537717192	0.0
20	0.0	0.0	0.0	1.7383177230219817	0.0
21	0.0	0.0	0.0	1.8324462942860382	0.0
22	0.0	0.0	0.0	1.9252717333658207	0.0
23	0.0	0.0	0.0	2.0451901997186446	0.0
24	0.0	0.0	0.0	2.1276511921235053	0.0
25	0.0	0.0	0.0	2.1968081140889195	0.0
26	0.0	0.0	0.0	2.272056421380823	0.0
27	0.0	0.0	0.0	2.338849522174764	0.0
28	0.0	0.0	0.0	2.412521948685685	0.0
29	0.0	0.0	0.0	2.4947708032931057	0.0
30	0.0	0.0	0.0	2.611476897284697	0.0
31	0.0	0.0	0.0	2.7254858107088373	0.0
32	0.0	0.0	0.0	2.8243117189162064	0.0
33	0.0	0.0	0.0	2.9236528189173576	0.0
34	0.0	0.0	0.0	3.0259941534823027	0.0
35	0.0	0.0	0.0	3.1882795685238423	0.0
36	0.0	0.0	0.0	3.303470392533719	0.0
37	0.0	0.0	0.0	3.424267715475937	0.0
38	0.0	0.0	0.0	3.539973731279597	0.0
39	0.0	0.0	0.0	3.657679903459119	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	65	0.0	45.000004	14
TCTAGCG	90	0.0	45.000004	1
CGTAGAT	30	2.166742E-6	45.000004	17
ATCGATA	45	3.8562575E-10	45.000004	15
ATAGCCG	55	1.8189894E-12	45.0	1
ACCGAAC	40	6.8212103E-9	45.0	11
CCGTACT	20	7.035208E-4	45.0	43
TACGCGA	35	1.2128476E-7	45.0	21
CGGATTA	20	7.035208E-4	45.0	38
GTTCACG	25	3.8923987E-5	45.0	44
CGTCGGT	20	7.035208E-4	45.0	39
TTACGAG	140	0.0	45.0	1
ACCAACG	20	7.035208E-4	45.0	38
TGGTCGA	80	0.0	45.0	10
TATTGCG	300	0.0	44.25	1
GTTTACG	85	0.0	42.35294	1
CTACGGG	425	0.0	42.35294	3
GTATGCG	85	0.0	42.35294	1
CGAGTAC	80	0.0	42.1875	40
ATGCTCG	160	0.0	42.1875	1
>>END_MODULE