FastQCFastQC Report
Thu 26 May 2016
SRR1545065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545065_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3191622
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179750.5631932603547664No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC81890.25657800328485014No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG207.0351723E-445.0000049
TATATCG253.8923703E-545.01
CGGTCTA5850.043.4615431
CGTTTTT162550.043.435871
CGACGGT5850.042.6923128
TATAGCG556.184564E-1140.909091
CGCATCG2050.040.60975621
ACGTAAG1350.040.01
CTCACGA6250.039.9624
TGGGCGA12050.039.7717866
GCGCGAC18150.039.5454569
ACCCGCT18350.039.4822934
CTATCGT403.460791E-739.37500419
GTACGTC403.460791E-739.37500419
AGGGTAC19400.039.201036
TAGTAGG19050.039.0944862
CATATGC30250.039.04958733
CGTAAGG4900.039.0306132
GACGGTC6450.038.72093229
CTACGTT356.251781E-638.57142630
Produced by FastQC (version 0.11.5)