##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545065_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3191622
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.856181590426434	31.0	31.0	33.0	28.0	34.0
2	31.136231671545065	31.0	31.0	34.0	28.0	34.0
3	31.213918502880354	31.0	31.0	34.0	28.0	34.0
4	35.05744038611088	35.0	35.0	37.0	32.0	37.0
5	34.908620444400995	35.0	35.0	37.0	32.0	37.0
6	34.96094681638365	36.0	35.0	37.0	32.0	37.0
7	35.53503391065734	37.0	35.0	37.0	35.0	37.0
8	35.46650574535455	37.0	35.0	37.0	35.0	37.0
9	37.16912309791072	39.0	37.0	39.0	34.0	39.0
10	36.490789636116055	38.0	35.0	39.0	32.0	39.0
11	36.19863097822988	38.0	35.0	39.0	31.0	39.0
12	35.37220385120794	37.0	35.0	39.0	30.0	39.0
13	34.88970091069682	37.0	34.0	39.0	27.0	39.0
14	35.861040561820914	38.0	34.0	40.0	28.0	41.0
15	36.24800931939935	38.0	35.0	40.0	30.0	41.0
16	36.343155298465796	38.0	35.0	40.0	31.0	41.0
17	36.21395892120057	38.0	34.0	40.0	31.0	41.0
18	36.141244169892296	37.0	35.0	40.0	30.0	41.0
19	36.04564544297539	37.0	34.0	40.0	30.0	41.0
20	35.89993332543766	37.0	34.0	40.0	30.0	41.0
21	35.72213501473546	37.0	34.0	40.0	30.0	41.0
22	35.60367957107702	36.0	34.0	40.0	29.0	41.0
23	35.56899093940323	36.0	34.0	40.0	29.0	41.0
24	35.48959745232988	36.0	34.0	40.0	29.0	41.0
25	35.35523379648342	36.0	34.0	40.0	29.0	41.0
26	35.17223217536412	36.0	34.0	40.0	29.0	41.0
27	35.08916594759655	36.0	34.0	40.0	27.0	41.0
28	35.11345140495961	36.0	34.0	40.0	27.0	41.0
29	35.14368305519889	36.0	34.0	40.0	29.0	41.0
30	35.01056516091191	36.0	34.0	40.0	27.0	41.0
31	34.828159788345864	36.0	33.0	40.0	27.0	41.0
32	34.60391863447489	36.0	33.0	40.0	25.0	41.0
33	34.35703382167437	36.0	33.0	40.0	24.0	41.0
34	34.177082060469566	36.0	33.0	40.0	23.0	41.0
35	33.989266899400995	35.0	33.0	40.0	23.0	41.0
36	33.81313764599943	35.0	33.0	40.0	23.0	41.0
37	33.76413184268062	35.0	33.0	40.0	23.0	41.0
38	33.72589485847635	35.0	33.0	40.0	23.0	41.0
39	33.67434896739025	35.0	33.0	40.0	23.0	41.0
40	33.516929009763686	35.0	32.0	40.0	22.0	41.0
41	33.4737412513136	35.0	32.0	40.0	22.0	41.0
42	33.43499731484493	35.0	32.0	40.0	22.0	41.0
43	33.30231148926784	35.0	32.0	39.0	22.0	41.0
44	33.23119999799475	35.0	32.0	39.0	22.0	41.0
45	33.17578710762114	35.0	32.0	39.0	22.0	41.0
46	33.137303853651844	35.0	32.0	39.0	22.0	41.0
47	33.02811642481472	35.0	32.0	39.0	21.0	41.0
48	33.00880806060367	35.0	32.0	39.0	21.0	41.0
49	33.03970833638821	35.0	32.0	39.0	21.0	41.0
50	32.92164610972101	35.0	32.0	39.0	21.0	40.0
51	32.73513530111022	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	4.0
13	11.0
14	56.0
15	144.0
16	455.0
17	1215.0
18	2925.0
19	5490.0
20	9117.0
21	13889.0
22	20614.0
23	29145.0
24	40190.0
25	54266.0
26	69529.0
27	80008.0
28	86659.0
29	95668.0
30	111685.0
31	132621.0
32	161863.0
33	202777.0
34	315036.0
35	413196.0
36	236298.0
37	280313.0
38	366338.0
39	461981.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.65028816069071	23.10301783857863	27.104713528105773	16.14198047262489
2	33.46395657129823	25.323926204293617	28.189209123135512	13.022908101272645
3	29.059738277277198	25.56443087558614	32.08804175431803	13.287789092818636
4	26.591432193411375	27.3204032307084	30.882322530675626	15.205842045204601
5	23.022024537993534	31.418444916095957	30.373521676439125	15.186008869471385
6	20.246351228309617	40.716820475607705	28.65329916888654	10.383529127196141
7	84.52811767809595	4.682039414441936	8.926370353381447	1.8634725540806523
8	85.79405706565501	3.5054903118226406	8.667035131353275	2.0334174911690672
9	81.69360281386706	5.127392905550845	10.217250037755097	2.961754242827001
10	47.768062759311725	23.855989211755027	16.145395664022868	12.23055236491038
11	42.23003225319289	20.933274679770975	22.58484870702107	14.251844360015065
12	37.379113190722464	21.274668491444164	26.711308544683547	14.634909773149829
13	21.180484405734763	36.455319583584775	27.45867148427978	14.905524526400683
14	15.583142364603328	38.84911809731854	31.34111746315823	14.2266220749199
15	14.812249069595335	22.082564915268787	47.856857735659176	15.248328279476706
16	17.517707297418053	17.86966000359692	45.336791136293705	19.27584156269132
17	17.38482815320862	17.634325117448117	31.216039994711153	33.76480673463211
18	21.996339165477615	21.921330282846778	35.57222628494227	20.510104266733343
19	28.822648797382648	22.803577616647587	27.539664784864875	20.834108801104893
20	30.51279255500808	22.025509286500718	26.814610251464615	20.647087907026584
21	23.22934858827267	26.200815760763653	29.743340533434097	20.826495117529582
22	22.548472218827918	23.29097869359216	27.647885620540276	26.512663467039644
23	19.757164225588117	27.54853174968715	27.96327384633895	24.731030178385787
24	20.28420032196795	23.07463101833488	38.58367312921141	18.057495530485753
25	19.407060109248526	23.249589080411152	35.80865779218216	21.534693018158166
26	17.667286414243293	31.37223017011413	28.970473320462137	21.990010095180445
27	18.175805280199224	30.50524153549512	30.566370328315823	20.752582855989836
28	16.314870620643674	27.54442725360334	37.89186188088689	18.24884024486609
29	17.69589255870526	24.27793767557687	37.25804622226567	20.7681235434522
30	19.607303120482314	27.89653661993807	33.22486184140854	19.271298418171074
31	28.693905481288194	26.08927372978379	26.01266691356307	19.20415387536494
32	29.394051049905034	25.730709965027188	27.29251772296343	17.582721262104346
33	27.52017626147457	26.14329015152797	25.92202334737635	20.41451023962111
34	20.55929555567671	28.082554889018812	28.43789145456448	22.92025810074
35	20.630419266441955	25.812956546859244	31.089051272362454	22.467572914336348
36	29.32123540945638	25.71391599631786	26.44401498673715	18.52083360748861
37	21.41854517859571	31.433076974654266	28.337096310277342	18.811281536472677
38	22.268896504661267	32.37639043721343	23.8125630165477	21.542150041577603
39	21.156170749543648	30.357886992883245	26.37207664316138	22.113865614411733
40	23.766504930721748	25.266024610683846	27.043271414973326	23.92419904362108
41	18.746267571786383	24.560677924892108	28.618645942407966	28.074408560913543
42	22.487594082256606	26.06950321811292	24.65871585043592	26.784186849194548
43	22.36458452786702	25.93533946062535	26.70125096267666	24.99882504883097
44	20.25709811500234	28.567449403469457	29.04234899997556	22.13310348155264
45	18.738967208522812	34.169271925058794	25.14035183364446	21.95140903277393
46	22.393973973108345	30.631133636752722	26.943948876151374	20.03094351398756
47	21.781589423810214	28.44002203268432	26.756238677387234	23.02214986611823
48	22.683231284907798	25.774825464920344	29.896992814311968	21.644950435859887
49	21.91616049770305	25.409149329087217	31.30367568590516	21.371014487304574
50	20.28532827509022	31.727566735659803	27.40662898049957	20.58047600875041
51	18.857088966049236	33.38863436835565	25.98246283551122	21.77181383008389
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2749.0
1	4395.5
2	6042.0
3	20941.0
4	35840.0
5	26842.0
6	17844.0
7	18449.0
8	19054.0
9	20134.5
10	21215.0
11	21310.5
12	21406.0
13	20634.5
14	19863.0
15	18598.5
16	17334.0
17	16369.5
18	15405.0
19	15297.0
20	15189.0
21	14798.0
22	14407.0
23	14561.0
24	14715.0
25	17076.5
26	21317.5
27	23197.0
28	27509.0
29	31821.0
30	36301.0
31	40781.0
32	50717.5
33	60654.0
34	67244.0
35	73834.0
36	80323.5
37	86813.0
38	100415.0
39	114017.0
40	157639.5
41	201262.0
42	244875.5
43	288489.0
44	288574.5
45	288660.0
46	282262.0
47	275864.0
48	260509.5
49	245155.0
50	230252.5
51	215350.0
52	203177.0
53	191004.0
54	176818.5
55	162633.0
56	162264.5
57	161896.0
58	154934.0
59	147972.0
60	131982.0
61	115992.0
62	103478.5
63	90965.0
64	73745.0
65	56525.0
66	43799.0
67	31073.0
68	25080.0
69	19087.0
70	15468.0
71	11849.0
72	10011.0
73	8173.0
74	6442.0
75	3226.5
76	1742.0
77	1310.5
78	879.0
79	655.5
80	432.0
81	292.5
82	153.0
83	116.5
84	80.0
85	64.0
86	48.0
87	27.5
88	7.0
89	4.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3191622.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.255270696037385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.37892332618092	14.042153509453229
2	6.465394314849243	2.2312425811868972
3	2.302488329063701	1.1919017817738289
4	1.3119246406606788	0.9055045922960636
5	0.8290723220387711	0.715293367168564
6	0.6040314973239482	0.6253636197154504
7	0.46586784707259066	0.562707306687239
8	0.3704657573638911	0.5113989541541157
9	0.28825826742627225	0.44765769913299575
>10	3.3868616302126444	13.81636160152873
>50	1.0868576664251748	13.501706266257784
>100	1.4902625612685687	48.00562109794142
>500	0.01574671261459557	1.7169467931819595
>1k	0.0034789248799687884	0.8994990192276906
>5k	1.831013094720415E-4	0.2587283972060259
>10k+	1.831013094720415E-4	0.5679134130880835
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17975	0.5631932603547664	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8189	0.25657800328485014	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02146244135427065	0.0
2	0.0	0.0	0.0	0.07792276153003082	0.0
3	0.0	0.0	0.0	0.11229399972803797	0.0
4	0.0	0.0	0.0	0.1603886675803087	0.0
5	0.0	0.0	0.0	0.26629093294882666	0.0
6	0.0	0.0	0.0	0.38450668656877285	0.0
7	0.0	0.0	0.0	0.45672701842511426	0.0
8	0.0	0.0	0.0	0.6549961116949313	0.0
9	0.0	0.0	0.0	0.7255245138678703	0.0
10	0.0	0.0	0.0	0.8241264159728189	0.0
11	0.0	0.0	0.0	0.9330992203964004	0.0
12	0.0	0.0	0.0	1.0417273724770666	0.0
13	0.0	0.0	0.0	1.088976075487636	0.0
14	0.0	0.0	0.0	1.1088405832520267	0.0
15	0.0	0.0	0.0	1.1402039464573186	0.0
16	0.0	0.0	0.0	1.2085077744168953	0.0
17	0.0	0.0	0.0	1.284049301577693	0.0
18	0.0	0.0	0.0	1.4190590239069665	0.0
19	0.0	0.0	0.0	1.464177148797696	0.0
20	0.0	0.0	0.0	1.5180682424171785	0.0
21	0.0	0.0	0.0	1.5842728242880892	0.0
22	0.0	0.0	0.0	1.6491614608496872	0.0
23	0.0	0.0	0.0	1.727115554410892	0.0
24	0.0	0.0	0.0	1.7858004488000145	0.0
25	0.0	0.0	0.0	1.83355046430937	0.0
26	0.0	0.0	0.0	1.887284897772982	0.0
27	3.1332031174117735E-5	0.0	0.0	1.9388260890544056	0.0
28	3.1332031174117735E-5	0.0	0.0	1.9964456943836082	0.0
29	3.1332031174117735E-5	0.0	0.0	2.054221959868681	0.0
30	3.1332031174117735E-5	0.0	0.0	2.13114209640114	0.0
31	3.1332031174117735E-5	0.0	0.0	2.206965611842505	0.0
32	3.1332031174117735E-5	0.0	0.0	2.2741728187109875	0.0
33	3.1332031174117735E-5	0.0	0.0	2.3458918380685434	0.0
34	6.266406234823547E-5	0.0	0.0	2.4167962246155716	0.0
35	9.39960935223532E-5	0.0	0.0	2.504463247840753	0.0
36	9.39960935223532E-5	0.0	0.0	2.5817907007784755	0.0
37	9.39960935223532E-5	0.0	0.0	2.6586795052797605	0.0
38	9.39960935223532E-5	0.0	0.0	2.738638848836109	0.0
39	9.39960935223532E-5	0.0	0.0	2.8222640400398293	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	20	7.0351723E-4	45.000004	9
TATATCG	25	3.8923703E-5	45.0	1
CGGTCTA	585	0.0	43.46154	31
CGTTTTT	16255	0.0	43.43587	1
CGACGGT	585	0.0	42.69231	28
TATAGCG	55	6.184564E-11	40.90909	1
CGCATCG	205	0.0	40.609756	21
ACGTAAG	135	0.0	40.0	1
CTCACGA	625	0.0	39.96	24
TGGGCGA	1205	0.0	39.771786	6
GCGCGAC	1815	0.0	39.545456	9
ACCCGCT	1835	0.0	39.48229	34
CTATCGT	40	3.460791E-7	39.375004	19
GTACGTC	40	3.460791E-7	39.375004	19
AGGGTAC	1940	0.0	39.20103	6
TAGTAGG	1905	0.0	39.094486	2
CATATGC	3025	0.0	39.049587	33
CGTAAGG	490	0.0	39.030613	2
GACGGTC	645	0.0	38.720932	29
CTACGTT	35	6.251781E-6	38.571426	30
>>END_MODULE