FastQCFastQC Report
Thu 26 May 2016
SRR1545064_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545064_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4361558
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238510.5468458747997849No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC95050.21792671334417654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA207.035457E-445.00000413
CGCACGA253.8926046E-545.00000440
CGTTTTT221550.043.314151
CGCATCG1250.043.221
CGACGGT6100.041.6803328
TGCGTAG4250.041.2941171
ACGGGAT16200.040.6944435
CGGTCTA6250.040.3231
TAATGCG2350.040.2127651
GCGATAT3250.039.461549
CGTTAGG7300.039.4520572
TCACGAC6450.039.41860225
CTATGCG1500.039.01
CGGGATC8700.038.7931066
AGGGATC45200.038.628326
GCGATCG356.252307E-638.5714269
TAACGGG9100.038.324183
TACGGGA12600.038.2142874
GTACGGG18400.038.1521763
TAGGGAC31800.038.0660365
Produced by FastQC (version 0.11.5)