FastQCFastQC Report
Thu 26 May 2016
SRR1545063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545063_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3885047
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195740.5038291686046527No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC124490.32043370389084097No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGAT207.035361E-445.00000438
CGTCCCG207.035361E-445.00000443
CGTTATC253.8925275E-545.020
GTATCGC253.8925275E-545.09
TCGACGA253.8925275E-545.039
CGACGAT302.166842E-644.99999634
CGGTCCG2250.044.029
CGACGGT3850.043.83116528
CGTTTTT205050.043.321141
TCACGAC3950.043.2911425
GGTCGAA950.042.631589
CTACGCG800.042.1875041
CTCGTAG1400.041.7857131
CGTTAGG6900.041.7391322
CGTAGGT603.6379788E-1241.24999624
CGCGAGG5750.041.086962
GCGTAAG2300.041.086961
GCGATAT6300.041.071439
ACGGTCC556.184564E-1140.90909226
GGCGTAT501.0822987E-940.545
Produced by FastQC (version 0.11.5)