##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545063_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3885047
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.971501245673476	31.0	31.0	33.0	30.0	34.0
2	31.242091022322253	31.0	31.0	34.0	28.0	34.0
3	31.30948402940814	31.0	31.0	34.0	28.0	34.0
4	35.12486721524862	35.0	35.0	37.0	32.0	37.0
5	34.99275658698595	35.0	35.0	37.0	32.0	37.0
6	35.045324805594376	37.0	35.0	37.0	32.0	37.0
7	35.67039188972489	37.0	35.0	37.0	35.0	37.0
8	35.64386351053153	37.0	35.0	37.0	35.0	37.0
9	37.387838036451036	39.0	37.0	39.0	35.0	39.0
10	36.65840541954834	39.0	35.0	39.0	32.0	39.0
11	36.228308692275796	38.0	35.0	39.0	31.0	39.0
12	35.44080779460326	37.0	35.0	39.0	30.0	39.0
13	34.958283644959764	37.0	34.0	39.0	28.0	39.0
14	35.95095400390266	38.0	34.0	40.0	29.0	41.0
15	36.365374472947174	38.0	35.0	40.0	31.0	41.0
16	36.47296802329547	38.0	35.0	40.0	31.0	41.0
17	36.3702403085471	38.0	35.0	40.0	31.0	41.0
18	36.30718650250563	38.0	35.0	40.0	31.0	41.0
19	36.22554990969221	37.0	35.0	40.0	31.0	41.0
20	36.09486140064715	37.0	34.0	40.0	31.0	41.0
21	35.92146632975096	37.0	34.0	40.0	30.0	41.0
22	35.82482940360824	37.0	34.0	40.0	30.0	41.0
23	35.79898081027076	36.0	34.0	40.0	30.0	41.0
24	35.733884557895955	36.0	34.0	40.0	30.0	41.0
25	35.62307817640301	36.0	34.0	40.0	30.0	41.0
26	35.49135029769267	36.0	34.0	40.0	29.0	41.0
27	35.3696681146972	36.0	34.0	40.0	29.0	41.0
28	35.44820667549196	36.0	34.0	40.0	29.0	41.0
29	35.536982435476325	36.0	34.0	40.0	30.0	41.0
30	35.431928365345385	36.0	34.0	40.0	29.0	41.0
31	35.219804290655944	36.0	34.0	40.0	29.0	41.0
32	34.98983718858485	36.0	34.0	40.0	27.0	41.0
33	34.75802429159802	36.0	33.0	40.0	26.0	41.0
34	34.61686795552281	36.0	34.0	40.0	25.0	41.0
35	34.42198459889932	36.0	33.0	40.0	24.0	41.0
36	34.23095061655625	36.0	33.0	40.0	23.0	41.0
37	34.20176564144526	36.0	33.0	40.0	23.0	41.0
38	34.18937248378205	36.0	33.0	40.0	23.0	41.0
39	34.14723013646939	36.0	33.0	40.0	23.0	41.0
40	34.02573096284292	36.0	33.0	40.0	23.0	41.0
41	34.012409373683255	36.0	33.0	40.0	23.0	41.0
42	33.98546117974892	36.0	33.0	40.0	23.0	41.0
43	33.878364148490355	35.0	33.0	40.0	23.0	41.0
44	33.821457243631805	35.0	33.0	40.0	23.0	41.0
45	33.76597657634515	35.0	33.0	40.0	23.0	41.0
46	33.72665324254765	35.0	33.0	40.0	23.0	41.0
47	33.648389067107814	35.0	33.0	40.0	23.0	41.0
48	33.62023599714495	35.0	33.0	40.0	23.0	41.0
49	33.66139843353246	36.0	33.0	40.0	23.0	41.0
50	33.53232715073975	35.0	33.0	39.0	23.0	41.0
51	33.3376134188338	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	5.0
13	6.0
14	24.0
15	120.0
16	410.0
17	1147.0
18	2696.0
19	5160.0
20	8772.0
21	14091.0
22	21019.0
23	30348.0
24	43051.0
25	59936.0
26	76103.0
27	86333.0
28	92808.0
29	104554.0
30	123314.0
31	151763.0
32	189112.0
33	240823.0
34	383941.0
35	519091.0
36	277420.0
37	333026.0
38	461087.0
39	658673.0
40	210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.58005553085973	22.855759531351875	29.700747507044316	15.863437430744082
2	31.333932382285205	25.738427360080845	30.766500379532086	12.16113987810186
3	27.379205451053746	25.506255136681744	34.31520905667293	12.799330355591579
4	24.348920360551624	28.45082697841236	33.3781290161998	13.822123644836214
5	22.925050842370762	31.870476727823373	32.2026477414559	13.001824688349975
6	20.08655236345918	40.904781846912016	28.702844521572068	10.30582126805673
7	87.66277473605854	3.824586935499107	7.5243877358497855	0.9882505925925735
8	89.59925066543597	2.610212952378697	6.854048355142164	0.9364880270431735
9	85.55196372141701	4.949206534695719	7.927265744790217	1.5715639990970507
10	47.63363223147622	32.521099487342106	12.605098471138188	7.240169810043483
11	40.479973601348966	20.7314866461075	27.351921353847196	11.436618398696336
12	36.48650840002708	23.742209553706815	27.178950473443436	12.59233157282267
13	20.242535032394716	40.01174245768455	26.726832390959494	13.018890118961238
14	14.868726169850714	41.62291987715979	30.132428256337697	13.375925696651802
15	13.726629304613303	24.7199583428463	48.328655998241466	13.22475635429893
16	16.365490559058873	21.588670613251267	45.08583808638609	16.960000741303773
17	16.701574009271962	21.2402321001522	30.974837627446977	31.083356263128863
18	20.744227804708668	23.945038502751704	36.390988320089825	18.919745372449807
19	27.150971403949555	24.482071902862437	29.298847607249023	19.068109085938985
20	28.78603527833769	23.803907648993693	28.93370401953953	18.47635305312909
21	20.97511304239048	28.336542646717017	31.40034084529737	19.28800346559514
22	21.616778381316877	24.379061566050552	29.575884152752852	24.42827589987972
23	17.04625452407654	30.352374115422542	29.81796616617508	22.783405194325834
24	17.3604592170957	25.02147335669298	41.63795187033774	15.980115555873583
25	15.523184146806976	26.671466265401676	39.139964072506714	18.66538551528463
26	16.485000052766413	35.59030302593508	29.725946687388856	18.198750233909657
27	16.083975303258878	35.31429606900509	31.39287632813709	17.208852299598952
28	13.944001192263569	29.397353494050392	40.65876165719488	15.999883656491157
29	14.67853542054961	25.263349452400448	40.05151031634881	20.00660481070113
30	17.548565049534794	30.634378425795106	33.798947606039256	18.018108918630844
31	26.614684455554848	26.901527832224424	28.824670589570733	17.659117122649995
32	26.871541065011566	27.439693779766372	29.97474676625534	15.714018388966721
33	26.01178312643322	28.05747266377987	28.0903680187138	17.84037619107311
34	18.908111021565503	28.49028596050447	30.4043940781154	22.19720893981463
35	19.60331496633117	26.703100374332667	33.28201692283259	20.411567736503574
36	27.94751260409462	25.997754982114756	28.623720639673085	17.431011774117533
37	19.406637809014924	32.63456014817839	30.840502058276257	17.118299984530434
38	19.519454976992556	33.20667677894244	26.407659933071592	20.866208310993407
39	20.045960833935858	31.967644149478758	29.372566149135395	18.613828867449993
40	23.211533863039495	27.433413289465996	26.86832360071834	22.486729246776164
41	17.003552337976863	26.23818965381886	29.17403573238625	27.58422227581803
42	21.189705040891397	26.465780208064405	26.64420790790948	25.70030684313472
43	21.24126168872603	27.83714070897984	27.540490501144514	23.381107101149613
44	18.922756919028263	31.114089482057743	30.25731735034351	19.70583624857048
45	17.194412319850958	37.89650421217555	24.899647288694318	20.00943617927917
46	21.17982099058261	33.059136736312325	27.725507567862113	18.03553470524295
47	20.826852287758683	28.03410100315389	28.812289786970403	22.32675692211703
48	22.162177188589997	25.53065638588156	32.79968041570668	19.507486009821758
49	21.4629063689577	25.41961525819379	32.65852382223432	20.458954550614187
50	19.234542078898915	33.98610106904756	27.72218714471151	19.057169707342023
51	18.19597034476031	33.569426573217775	26.816483816025904	21.418119265996012
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3440.0
1	4223.5
2	5007.0
3	20049.0
4	35091.0
5	27045.0
6	18999.0
7	20167.5
8	21336.0
9	22951.5
10	24567.0
11	25152.0
12	25737.0
13	25468.0
14	25199.0
15	23656.0
16	22113.0
17	21188.0
18	20263.0
19	20302.5
20	20342.0
21	21049.5
22	21757.0
23	23958.0
24	26159.0
25	29780.0
26	36046.0
27	38691.0
28	50168.5
29	61646.0
30	72868.5
31	84091.0
32	99047.0
33	114003.0
34	129389.5
35	144776.0
36	148637.5
37	152499.0
38	180583.0
39	208667.0
40	263802.5
41	318938.0
42	361103.0
43	403268.0
44	418687.0
45	434106.0
46	399766.5
47	365427.0
48	332731.0
49	300035.0
50	277895.0
51	255755.0
52	229028.0
53	202301.0
54	181962.5
55	161624.0
56	139181.0
57	116738.0
58	103387.0
59	90036.0
60	74009.5
61	57983.0
62	46316.0
63	34649.0
64	26773.5
65	18898.0
66	14048.5
67	9199.0
68	6542.5
69	3886.0
70	2941.0
71	1996.0
72	1568.5
73	1141.0
74	901.0
75	488.0
76	315.0
77	221.0
78	127.0
79	110.5
80	94.0
81	74.5
82	55.0
83	34.0
84	13.0
85	8.0
86	3.0
87	3.0
88	3.0
89	2.0
90	1.0
91	2.0
92	3.0
93	5.0
94	7.0
95	4.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3885047.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.670995927927258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.16774125980756	12.719793427614976
2	7.756623608469574	2.4310803396558223
3	2.6104904322154088	1.2272695479942222
4	1.2946565484767798	0.8115422999697589
5	0.7525550845956273	0.5896643833119515
6	0.5077791070760028	0.4774442591564748
7	0.3592079021060986	0.39404018998287754
8	0.2761655449035548	0.3462231303693936
9	0.21779661354019453	0.3071780859514261
>10	2.4148508023682314	8.899491452418818
>50	0.941439364918921	10.696968952443774
>100	1.6622562911656882	55.999321525929936
>500	0.034295474800801744	3.4707214717786234
>1k	0.0038106083112001933	0.7978320885888023
>5k	0.0	0.0
>10k+	3.3135724445219075E-4	0.8314288448331939
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19574	0.5038291686046527	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	12449	0.32043370389084097	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019124607758928013	0.0
2	0.0	0.0	0.0	0.07034663930706629	0.0
3	0.0	0.0	0.0	0.10110559795029507	0.0
4	0.0	0.0	0.0	0.1450175506242267	0.0
5	0.0	0.0	0.0	0.2439867522838205	0.0
6	0.0	0.0	0.0	0.345169569377153	0.0
7	0.0	0.0	0.0	0.4095960743846857	0.0
8	0.0	0.0	0.0	0.5834678447905521	0.0
9	0.0	0.0	0.0	0.6673278341291624	0.0
10	0.0	0.0	0.0	0.8123453847533891	0.0
11	0.0	0.0	0.0	0.95427416965612	0.0
12	0.0	0.0	0.0	1.085083397961466	0.0
13	0.0	0.0	0.0	1.1395228938028292	0.0
14	0.0	0.0	0.0	1.1608868567098416	0.0
15	0.0	0.0	0.0	1.1956097313623233	0.0
16	0.0	0.0	0.0	1.2772303655528492	0.0
17	0.0	0.0	0.0	1.3798803463638922	0.0
18	2.5739714345798133E-5	0.0	0.0	1.5368153847302233	0.0
19	2.5739714345798133E-5	0.0	0.0	1.6078055168959346	0.0
20	2.5739714345798133E-5	0.0	0.0	1.6849989202189832	0.0
21	2.5739714345798133E-5	0.0	0.0	1.7769411798621741	0.0
22	5.1479428691596266E-5	0.0	0.0	1.8735423278019545	0.0
23	7.72191430373944E-5	0.0	0.0	1.987466303496457	0.0
24	7.72191430373944E-5	0.0	0.0	2.0722014431228244	0.0
25	7.72191430373944E-5	0.0	0.0	2.1418016307138625	0.0
26	7.72191430373944E-5	0.0	0.0	2.210217791444994	0.0
27	7.72191430373944E-5	0.0	0.0	2.2811564441820136	0.0
28	7.72191430373944E-5	0.0	0.0	2.3597140523653897	0.0
29	7.72191430373944E-5	0.0	0.0	2.447048903140683	0.0
30	7.72191430373944E-5	0.0	0.0	2.5679483414228965	0.0
31	7.72191430373944E-5	0.0	0.0	2.6712675548069305	0.0
32	7.72191430373944E-5	0.0	0.0	2.766144141885542	0.0
33	7.72191430373944E-5	0.0	0.0	2.862951207540089	0.0
34	7.72191430373944E-5	0.0	0.0	2.970568953219871	0.0
35	7.72191430373944E-5	0.0	0.0	3.098340895232413	0.0
36	7.72191430373944E-5	0.0	0.0	3.212084692926495	0.0
37	7.72191430373944E-5	0.0	0.0	3.3289172563420726	0.0
38	7.72191430373944E-5	0.0	0.0	3.4410137123180236	0.0
39	7.72191430373944E-5	0.0	0.0	3.5635862320327143	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCGAT	20	7.035361E-4	45.000004	38
CGTCCCG	20	7.035361E-4	45.000004	43
CGTTATC	25	3.8925275E-5	45.0	20
GTATCGC	25	3.8925275E-5	45.0	9
TCGACGA	25	3.8925275E-5	45.0	39
CGACGAT	30	2.166842E-6	44.999996	34
CGGTCCG	225	0.0	44.0	29
CGACGGT	385	0.0	43.831165	28
CGTTTTT	20505	0.0	43.32114	1
TCACGAC	395	0.0	43.29114	25
GGTCGAA	95	0.0	42.63158	9
CTACGCG	80	0.0	42.187504	1
CTCGTAG	140	0.0	41.785713	1
CGTTAGG	690	0.0	41.739132	2
CGTAGGT	60	3.6379788E-12	41.249996	24
CGCGAGG	575	0.0	41.08696	2
GCGTAAG	230	0.0	41.08696	1
GCGATAT	630	0.0	41.07143	9
ACGGTCC	55	6.184564E-11	40.909092	26
GGCGTAT	50	1.0822987E-9	40.5	45
>>END_MODULE