FastQCFastQC Report
Thu 26 May 2016
SRR1545062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545062_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1932543
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86490.4475450222841096No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC53290.2757506560009273No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT21940.11352916856183794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC302.1662727E-645.0000041
ATCCGTT253.8917955E-545.00000439
CGTTACA253.8917955E-545.00000426
CTACGTC253.8917955E-545.00000442
GTATCGC302.1662727E-645.00000437
ACGTCGT253.8917955E-545.00000419
TAGTCGA253.8917955E-545.00000410
GCGTAAG1400.045.0000041
ACGTTAG551.8189894E-1245.01
TATGCGA453.8562575E-1045.022
AATGCGC207.0344826E-445.026
TCGCTAA207.0344826E-445.013
ACTACGT207.0344826E-445.010
CATCGTA207.0344826E-445.040
CGGTATG551.8189894E-1245.06
GTATGCG1450.043.4482731
ACGTTGT1350.043.33333628
CGTTTTT84400.043.000591
TAGTACG850.042.3529431
AATACGG1500.042.02