FastQCFastQC Report
Thu 26 May 2016
SRR1545061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545061_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2894496
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140140.48416028213547363No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC84750.29279708799044807No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG42590.14714133306800217No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC31900.11020916940289432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTA302.1666492E-645.00000426
TACGATG302.1666492E-645.0000041
TCCGTCG302.1666492E-645.00000433
CGAACTA207.0350646E-445.016
ACGTTCC207.0350646E-445.033
TAGACGT253.8922797E-545.021
TACGCAT207.0350646E-445.045
ATCGGGT351.212793E-745.012
CGTCCTA253.8922797E-545.041
TCGAATC207.0350646E-445.043
AATCGTC207.0350646E-445.021
CCCGATA253.8922797E-545.040
TCGTAAG1200.043.1250041
CGTTTTT136750.042.9926871
TCGTACA1750.042.4285734
TATAGCG850.042.352941
ATGACGG4050.042.2222252
GTACGTA1600.042.187511
ACGGGAT6150.042.073175
CGTAAGG5100.041.9117662