##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545061_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2894496
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.053997656241364	31.0	31.0	33.0	30.0	34.0
2	31.346935010447414	31.0	31.0	34.0	30.0	34.0
3	31.426596892861486	31.0	31.0	34.0	30.0	34.0
4	35.23751699777785	37.0	35.0	37.0	33.0	37.0
5	35.109146462803885	37.0	35.0	37.0	32.0	37.0
6	35.17165751826916	37.0	35.0	37.0	32.0	37.0
7	35.663768061866385	37.0	35.0	37.0	35.0	37.0
8	35.60750265331166	37.0	35.0	37.0	35.0	37.0
9	37.33046409461267	39.0	37.0	39.0	35.0	39.0
10	36.736368265839715	39.0	37.0	39.0	32.0	39.0
11	36.42944125678529	38.0	35.0	39.0	32.0	39.0
12	35.33024954948979	37.0	35.0	39.0	30.0	39.0
13	34.710299133251524	37.0	33.0	39.0	27.0	39.0
14	35.63354311078682	37.0	34.0	40.0	27.0	41.0
15	36.09417287154655	37.0	34.0	40.0	30.0	41.0
16	36.26122060628172	37.0	35.0	40.0	31.0	41.0
17	36.21302845123987	37.0	34.0	40.0	31.0	41.0
18	36.17418956529911	37.0	35.0	40.0	31.0	41.0
19	36.06895259140106	37.0	35.0	40.0	31.0	41.0
20	35.88962258023504	36.0	34.0	40.0	31.0	41.0
21	35.71354425779134	36.0	34.0	40.0	30.0	41.0
22	35.60934787956176	35.0	34.0	40.0	30.0	41.0
23	35.623039036847864	35.0	34.0	40.0	30.0	41.0
24	35.52225257868727	35.0	34.0	40.0	30.0	41.0
25	35.418243452400695	35.0	34.0	40.0	30.0	41.0
26	35.29933086796458	35.0	34.0	40.0	29.0	41.0
27	35.184343664665626	35.0	34.0	40.0	29.0	41.0
28	35.2604857633246	36.0	34.0	40.0	29.0	41.0
29	35.33136787889843	36.0	34.0	40.0	30.0	41.0
30	35.24197338673123	36.0	34.0	40.0	29.0	41.0
31	35.02419523122506	35.0	34.0	40.0	29.0	41.0
32	34.76514426000243	35.0	34.0	40.0	27.0	41.0
33	34.534803641117485	35.0	33.0	40.0	27.0	41.0
34	34.412801399621905	35.0	34.0	40.0	26.0	41.0
35	34.24030124760925	35.0	33.0	40.0	25.0	41.0
36	34.00835827722685	35.0	33.0	40.0	23.0	41.0
37	33.953266129923826	35.0	33.0	40.0	23.0	41.0
38	33.97165689639923	35.0	33.0	40.0	23.0	41.0
39	33.92410215802682	35.0	33.0	40.0	23.0	41.0
40	33.75584315887809	35.0	33.0	40.0	23.0	41.0
41	33.77033410997977	35.0	33.0	39.0	23.0	41.0
42	33.7419644041657	35.0	33.0	39.0	23.0	41.0
43	33.608734301239316	35.0	33.0	39.0	23.0	41.0
44	33.5181969503499	35.0	33.0	39.0	23.0	41.0
45	33.46766276408743	35.0	33.0	39.0	23.0	41.0
46	33.438455606779215	35.0	33.0	39.0	23.0	41.0
47	33.39190657026301	35.0	32.0	39.0	23.0	41.0
48	33.401914530198006	35.0	33.0	39.0	23.0	41.0
49	33.435745635855085	35.0	33.0	39.0	24.0	41.0
50	33.333607992548615	35.0	32.0	39.0	23.0	41.0
51	33.13450735464827	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	7.0
13	14.0
14	31.0
15	90.0
16	256.0
17	755.0
18	1781.0
19	3385.0
20	5801.0
21	9683.0
22	14679.0
23	21412.0
24	30897.0
25	43798.0
26	56800.0
27	65793.0
28	70821.0
29	79438.0
30	93991.0
31	115441.0
32	145477.0
33	191300.0
34	326949.0
35	450956.0
36	191972.0
37	232252.0
38	312080.0
39	428403.0
40	232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.452866405757682	24.917256752125414	29.201940510541384	15.427936331575514
2	31.93664803820769	25.58614694924436	29.42239339767614	13.054811614871811
3	29.59064375974263	24.521954772091583	33.09733369816369	12.7900677700021
4	27.46004831238323	26.26771638309398	31.775791018540016	14.496444285982776
5	23.32478607674704	32.07525593402099	30.773060318618512	13.826897670613466
6	20.63509502172399	41.57870662111815	28.091349927586702	9.694848429571158
7	85.32013863553448	5.028682022707925	7.987608205366323	1.663571136391275
8	87.20823936187855	3.8073640454158517	7.459122417166926	1.5252741755386776
9	82.83193343504361	5.518819165754591	9.1323670856688	2.5168803135329947
10	54.0279205775375	21.29178965871779	15.04631548981239	9.633974273932317
11	50.222560335201706	18.84690806275082	19.14105253557096	11.789479066476513
12	44.68629426331907	20.51721612329055	22.9218834643406	11.874606149049782
13	21.245425801244846	42.267012979116224	22.89172277315291	13.59583844648602
14	14.61573966590384	44.1967789901938	27.44097763479376	13.746503709108598
15	14.22344339049009	22.307061402054103	49.734116060274395	13.735379147181408
16	14.103491592318662	16.709472046256067	49.1818610217461	20.00517533967917
17	14.565264557283895	18.063213768476448	30.171677556299958	37.1998441179397
18	20.58202878843156	22.730589366853504	36.23439106497297	20.452990779741963
19	30.602564315169207	22.831850519054093	26.11508186572032	20.450503300056383
20	31.386362254430477	22.322919085049694	25.653550739057852	20.637167921461973
21	21.269644179850307	28.2725559130156	29.31878295910583	21.139016948028257
22	21.74050335533371	24.935878301438315	25.557920964478793	27.76569737874918
23	18.653610162183675	30.547425182138788	25.425359026234617	25.373605629442913
24	18.782440880899472	22.88163466109471	41.32059605540999	17.015328402595824
25	16.67834400185732	23.90481797176434	38.36861408687385	21.048223939504492
26	15.287428277669065	34.52079394823831	28.732670558190442	21.459107215902183
27	16.624759543630393	34.913297513625864	30.000939714547886	18.461003228195857
28	14.767786861685073	29.222324024631575	40.262311642510475	15.747577471172875
29	15.465801300122717	24.512453981625818	39.414702939648215	20.607041778603254
30	17.666460758626027	30.441396360540836	34.02146694968658	17.87067593114656
31	30.485272738328195	26.712284280233934	25.297979337335413	17.50446364410246
32	31.134574032923172	26.454415552828543	26.460461510397664	15.95054890385062
33	28.59181702099433	27.1882220600754	24.445810255049583	19.77415066388069
34	18.90950963483798	28.363728953157995	28.699193227421976	24.027568184582048
35	20.6666721943993	24.633718616297966	32.389887565918215	22.309721623384522
36	31.21306783633489	25.09721899771152	26.018484737930198	17.671228428023394
37	19.396952008225266	34.09678230683338	29.13070185621262	17.375563828728733
38	20.957603672625563	34.40775181586017	23.436273534321693	21.198370977192575
39	21.12391932826993	32.113328192541985	26.722717875581793	20.040034603606294
40	23.723404696361648	26.258353785944085	25.924340541496687	24.09390097619758
41	17.155318231567776	24.523716736868874	28.300090931201836	30.02087410036151
42	21.939674471825146	25.813820437133096	23.44777812786751	28.798726963174243
43	22.447189424341925	25.985041955490694	25.372258244613228	26.195510375554154
44	19.07748361027274	30.930117022099875	28.42850706997004	21.563892297657347
45	16.74509137342045	38.84123522713453	23.474725824461323	20.938947574983693
46	21.52713287563707	34.11782223917393	25.916705360795113	18.438339524393886
47	22.191462693332447	28.62861099134357	26.80960692293235	22.37031939239163
48	22.897285054116505	25.111314715929822	30.991923982620804	20.99947624733287
49	20.672096282047033	25.57243126264469	33.02664781709838	20.7288246382099
50	19.400614131095708	34.00906409958763	27.173055343659136	19.417266425657523
51	18.421134456568605	35.31150155329287	24.250992228007917	22.01637176213061
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2336.0
1	3462.5
2	4589.0
3	15190.0
4	25791.0
5	19819.5
6	13848.0
7	15012.5
8	16177.0
9	17056.0
10	17935.0
11	18055.5
12	18176.0
13	17593.5
14	17011.0
15	15745.0
16	14479.0
17	13725.0
18	12971.0
19	12367.5
20	11764.0
21	12255.5
22	12747.0
23	13215.5
24	13684.0
25	15357.5
26	19370.5
27	21710.0
28	27913.0
29	34116.0
30	40450.5
31	46785.0
32	53032.0
33	59279.0
34	63307.0
35	67335.0
36	70019.5
37	72704.0
38	91558.5
39	110413.0
40	159880.0
41	209347.0
42	257858.0
43	306369.0
44	308338.0
45	310307.0
46	284830.0
47	259353.0
48	244315.0
49	229277.0
50	218093.0
51	206909.0
52	184859.0
53	162809.0
54	154565.0
55	146321.0
56	134961.5
57	123602.0
58	112089.5
59	100577.0
60	90964.5
61	81352.0
62	69437.5
63	57523.0
64	47949.0
65	38375.0
66	29939.5
67	21504.0
68	16983.5
69	12463.0
70	9640.0
71	6817.0
72	5896.0
73	4975.0
74	4065.5
75	2242.5
76	1329.0
77	1077.5
78	826.0
79	525.0
80	224.0
81	161.0
82	98.0
83	86.0
84	74.0
85	46.0
86	18.0
87	11.5
88	5.0
89	3.0
90	1.0
91	2.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2894496.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.778436709379623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.0357758232681	12.123605209090226
2	7.101139226822362	2.098874732561744
3	2.4038452000948842	1.0657522244624473
4	1.1877844328216434	0.7021438825936411
5	0.6996893953164585	0.5170157722454191
6	0.48012154081009917	0.4257267482203125
7	0.33537052239553883	0.3469376427589836
8	0.26523094033892236	0.3135758932134405
9	0.22469629657261297	0.29885939979573184
>10	2.595201770924017	9.003166305580535
>50	0.8782575963032123	9.40151194880919
>100	1.7454402184178666	57.605887530125074
>500	0.04040044642211963	3.7666214413396424
>1k	0.006576816859414823	1.5496712807760247
>5k	2.3488631640767224E-4	0.29418865453882603
>10k+	2.3488631640767224E-4	0.48646133388874424
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14014	0.48416028213547363	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8475	0.29279708799044807	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4259	0.14714133306800217	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	3190	0.11020916940289432	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014786684797629708	0.0
2	0.0	0.0	0.0	0.054240876477286544	0.0
3	0.0	0.0	0.0	0.07552264712060407	0.0
4	0.0	0.0	0.0	0.10727256144074823	0.0
5	0.0	0.0	0.0	0.17353625639835052	0.0
6	0.0	0.0	0.0	0.2523064464417985	0.0
7	0.0	0.0	0.0	0.305890904668723	0.0
8	0.0	0.0	0.0	0.4674734392446906	0.0
9	0.0	0.0	0.0	0.5427887963914961	0.0
10	0.0	0.0	0.0	0.6603567598642389	0.0
11	0.0	0.0	0.0	0.7590267873923474	0.0
12	0.0	0.0	0.0	0.8563494300907654	0.0
13	0.0	0.0	0.0	0.897980166495307	0.0
14	0.0	0.0	0.0	0.9130086895957016	0.0
15	0.0	0.0	0.0	0.9362597149901054	0.0
16	0.0	0.0	0.0	0.9933335544426387	0.0
17	0.0	0.0	0.0	1.054795031673908	0.0
18	0.0	0.0	0.0	1.1685972272893104	0.0
19	0.0	0.0	0.0	1.2047347793881906	0.0
20	3.454832896642455E-5	0.0	0.0	1.2495439620576432	0.0
21	3.454832896642455E-5	0.0	0.0	1.302921130310769	0.0
22	3.454832896642455E-5	0.0	0.0	1.354812720418339	0.0
23	3.454832896642455E-5	0.0	0.0	1.4225619935214973	0.0
24	3.454832896642455E-5	0.0	0.0	1.4730371021414437	0.0
25	3.454832896642455E-5	0.0	0.0	1.5145987418880524	0.0
26	3.454832896642455E-5	0.0	0.0	1.5564022199374261	0.0
27	3.454832896642455E-5	0.0	0.0	1.6002095010668524	0.0
28	3.454832896642455E-5	0.0	0.0	1.6433258156169503	0.0
29	3.454832896642455E-5	0.0	0.0	1.6903806396692205	0.0
30	3.454832896642455E-5	0.0	0.0	1.754882369849535	0.0
31	3.454832896642455E-5	0.0	0.0	1.815894718804241	0.0
32	3.454832896642455E-5	0.0	0.0	1.8722084950195128	0.0
33	3.454832896642455E-5	0.0	0.0	1.9316316208417632	0.0
34	3.454832896642455E-5	0.0	0.0	1.9928858080992338	0.0
35	3.454832896642455E-5	0.0	0.0	2.081191336937415	0.0
36	3.454832896642455E-5	0.0	0.0	2.144414778945972	0.0
37	3.454832896642455E-5	0.0	0.0	2.212198600378097	0.0
38	3.454832896642455E-5	0.0	0.0	2.2735909809514334	0.0
39	3.454832896642455E-5	0.0	0.0	2.3378854211579494	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTA	30	2.1666492E-6	45.000004	26
TACGATG	30	2.1666492E-6	45.000004	1
TCCGTCG	30	2.1666492E-6	45.000004	33
CGAACTA	20	7.0350646E-4	45.0	16
ACGTTCC	20	7.0350646E-4	45.0	33
TAGACGT	25	3.8922797E-5	45.0	21
TACGCAT	20	7.0350646E-4	45.0	45
ATCGGGT	35	1.212793E-7	45.0	12
CGTCCTA	25	3.8922797E-5	45.0	41
TCGAATC	20	7.0350646E-4	45.0	43
AATCGTC	20	7.0350646E-4	45.0	21
CCCGATA	25	3.8922797E-5	45.0	40
TCGTAAG	120	0.0	43.125004	1
CGTTTTT	13675	0.0	42.992687	1
TCGTACA	175	0.0	42.42857	34
TATAGCG	85	0.0	42.35294	1
ATGACGG	405	0.0	42.222225	2
GTACGTA	160	0.0	42.1875	11
ACGGGAT	615	0.0	42.07317	5
CGTAAGG	510	0.0	41.911766	2
>>END_MODULE