##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545060_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2794222
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.974765426655434	31.0	31.0	33.0	30.0	34.0
2	31.25712380762874	31.0	31.0	34.0	30.0	34.0
3	31.322239607303928	31.0	31.0	34.0	28.0	34.0
4	35.18139217284811	37.0	35.0	37.0	32.0	37.0
5	35.07158056875939	37.0	35.0	37.0	32.0	37.0
6	35.121162885411394	37.0	35.0	37.0	32.0	37.0
7	35.613166384059674	37.0	35.0	37.0	35.0	37.0
8	35.53670860797746	37.0	35.0	37.0	35.0	37.0
9	37.25896331787524	39.0	37.0	39.0	34.0	39.0
10	36.625759871620794	39.0	35.0	39.0	32.0	39.0
11	36.3438492002425	38.0	35.0	39.0	32.0	39.0
12	35.70099727222819	37.0	35.0	39.0	31.0	39.0
13	35.29625598825004	37.0	35.0	39.0	30.0	39.0
14	36.291254596091505	38.0	35.0	40.0	30.0	41.0
15	36.59733729102412	38.0	35.0	40.0	31.0	41.0
16	36.66643774188307	38.0	35.0	40.0	31.0	41.0
17	36.57292262390032	38.0	35.0	40.0	31.0	41.0
18	36.48329195031748	38.0	35.0	40.0	31.0	41.0
19	36.37992435819344	38.0	35.0	40.0	31.0	41.0
20	36.231124799675904	38.0	35.0	40.0	30.0	41.0
21	36.06776125876899	37.0	34.0	40.0	30.0	41.0
22	35.97281712047217	37.0	34.0	40.0	30.0	41.0
23	35.930380979034595	37.0	34.0	40.0	30.0	41.0
24	35.840446822049216	37.0	34.0	40.0	30.0	41.0
25	35.6985411323796	37.0	34.0	40.0	30.0	41.0
26	35.551937891835365	37.0	34.0	40.0	29.0	41.0
27	35.48696989716637	37.0	34.0	40.0	29.0	41.0
28	35.4817548498294	37.0	34.0	40.0	29.0	41.0
29	35.481208007094644	37.0	34.0	40.0	29.0	41.0
30	35.33125034446082	37.0	34.0	40.0	29.0	41.0
31	35.1473100562518	37.0	34.0	40.0	27.0	41.0
32	34.909260609930065	37.0	34.0	40.0	26.0	41.0
33	34.642952492679534	36.0	33.0	40.0	25.0	41.0
34	34.4305728034494	36.0	33.0	40.0	24.0	41.0
35	34.219758129454284	36.0	33.0	40.0	23.0	41.0
36	34.07167862825502	36.0	33.0	40.0	23.0	41.0
37	34.00897995935899	36.0	33.0	40.0	23.0	41.0
38	33.94295585676442	36.0	33.0	40.0	23.0	41.0
39	33.86349330869201	36.0	33.0	40.0	23.0	41.0
40	33.70580505056506	36.0	33.0	40.0	22.0	41.0
41	33.6352859579518	36.0	33.0	40.0	22.0	41.0
42	33.60679287472506	35.0	33.0	40.0	22.0	41.0
43	33.49236531671428	35.0	32.0	40.0	21.0	41.0
44	33.39428363243865	35.0	32.0	40.0	21.0	41.0
45	33.338902206052346	35.0	32.0	40.0	21.0	41.0
46	33.297843550011414	35.0	32.0	39.0	22.0	41.0
47	33.20036095915071	35.0	32.0	39.0	21.0	41.0
48	33.14717227192399	35.0	32.0	39.0	20.0	41.0
49	33.156673306559036	35.0	32.0	39.0	20.0	41.0
50	33.08863182667662	35.0	32.0	39.0	20.0	41.0
51	32.89045966999043	35.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	6.0
13	14.0
14	58.0
15	123.0
16	347.0
17	952.0
18	2138.0
19	4169.0
20	7225.0
21	11164.0
22	16379.0
23	23489.0
24	33012.0
25	46094.0
26	58842.0
27	67506.0
28	72253.0
29	78573.0
30	90723.0
31	108912.0
32	132496.0
33	166840.0
34	259340.0
35	329693.0
36	219193.0
37	265675.0
38	351855.0
39	447008.0
40	138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.40166887240885	22.52015051058935	26.200137283293884	16.878043333707915
2	35.364441336443555	24.444943887779853	27.793926180525386	12.3966885952512
3	29.04715516519446	24.374691774669298	32.989039525134366	13.58911353500187
4	25.863406701400248	27.218417147957464	31.95451184623126	14.963664304411031
5	22.670245957550975	30.744371778620312	32.072970580004025	14.512411683824691
6	20.82046451570419	39.26481145735736	29.875686326999073	10.039037699939374
7	83.91570175884378	4.372487225424465	9.857233963514709	1.8545770522170393
8	85.06582512055235	3.451300576690041	9.729434525961073	1.7534397767965466
9	80.6617727582132	4.983999123906404	11.407325545357526	2.94690257252287
10	45.097562040525055	24.37036141008123	18.182914600199986	12.349161949193729
11	39.00609901432313	22.64311854963564	24.218082886757028	14.132699549284203
12	34.475106129720544	21.99449435298985	28.511406752935166	15.018992764354444
13	22.726683849744223	31.78115411016018	28.8556886317551	16.636473408340496
14	17.767163811608384	35.025384525638984	31.676402232893448	15.531049429859186
15	17.09760355476408	23.28004002545252	43.23733046264756	16.38502595713583
16	19.515128003429936	19.3067694692834	42.126287746642895	19.051814780643774
17	19.51451960509938	19.26536259466857	31.90737886968179	29.312738930550257
18	22.232628617196486	22.540048714812208	34.89561674054531	20.331705927445995
19	28.235193910863206	23.574504817441134	28.346244500258035	19.844056771437632
20	29.133046694213988	23.153206867600357	27.833185766914724	19.88056067127093
21	24.030624624671912	25.89908747408044	30.95698910108073	19.113298800166916
22	23.434644777687673	22.593802496723598	29.493039565217078	24.478513160371655
23	20.60072535396257	27.148308187395276	28.648296377310032	23.602670081332118
24	20.834600829855322	23.48449765265609	37.01248504950573	18.66841646798286
25	20.944398834451952	23.461450092369184	34.77816007461111	20.81599099856776
26	18.384795481532965	29.337039075635364	30.345226685639147	21.93293875719252
27	19.269442442296995	29.046833071960638	31.167530711589848	20.51619377415252
28	17.981248447689556	26.899795363432112	36.636423304948565	18.482532883929768
29	19.212861397555383	24.245747116728737	35.897720367243544	20.643671118472334
30	20.320504240536362	26.47835426104297	34.092244639116004	19.108896859304664
31	27.3235626947322	25.28897131294507	28.03542452961862	19.352041462704108
32	28.10485351557607	24.926902729990672	29.009541833111328	17.95870192132193
33	26.77768623967602	25.63507838675667	27.45157686110839	20.135658512458924
34	21.12265954530456	27.57440174760631	29.320540744436197	21.98239796265293
35	20.728524791516207	25.978107680778407	31.45229691842667	21.841070609278717
36	27.890912032043268	25.669757091598306	26.86715658240469	19.572174293953736
37	22.08228265327522	30.525849413539795	28.35887771265132	19.03299022053366
38	22.517072730799487	31.135357176344613	25.411939351991357	20.935630740864543
39	21.734099867512317	28.966023458408102	27.057263166634577	22.242613507445004
40	23.92261602692986	25.810833928012876	27.62794080069515	22.638609244362115
41	20.005425481583067	24.903783593429583	29.13605289772967	25.95473802725768
42	22.558157512180493	26.587830172405774	25.655942870681	25.19806944473274
43	22.05250692321512	26.37617912964682	27.131451974825193	24.439861972312865
44	20.88302217934008	27.838231894244625	29.451203232957152	21.827542693458142
45	20.03520121164317	32.50575652185116	26.393751104958735	21.065291161546934
46	22.235634820712168	30.011860188632113	27.238744809825416	20.5137601808303
47	22.002224590601603	28.15735471268926	27.914460626249454	21.925960070459684
48	22.93847804505154	26.459708641618313	29.859474300896636	20.742339012433515
49	22.09827995055511	25.990776681308787	30.72404411675236	21.186899251383746
50	20.81452368494701	29.995397645570037	28.43485592769651	20.755222741786444
51	19.966273259605	31.40605864530449	27.273924548586333	21.353743546504177
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1786.0
1	3488.0
2	5190.0
3	23623.0
4	42056.0
5	30706.5
6	19357.0
7	19493.0
8	19629.0
9	20038.0
10	20447.0
11	20199.0
12	19951.0
13	19580.0
14	19209.0
15	17836.0
16	16463.0
17	15695.5
18	14928.0
19	14327.5
20	13727.0
21	14017.0
22	14307.0
23	15457.0
24	16607.0
25	16301.5
26	19826.0
27	23656.0
28	26664.0
29	29672.0
30	34727.5
31	39783.0
32	45341.0
33	50899.0
34	60775.0
35	70651.0
36	77718.5
37	84786.0
38	98258.5
39	111731.0
40	136797.5
41	161864.0
42	186157.0
43	210450.0
44	219154.0
45	227858.0
46	221725.0
47	215592.0
48	211194.0
49	206796.0
50	197812.0
51	188828.0
52	179289.0
53	169750.0
54	161507.5
55	153265.0
56	149178.0
57	145091.0
58	137434.5
59	129778.0
60	117565.0
61	105352.0
62	92736.0
63	80120.0
64	68867.5
65	57615.0
66	46009.5
67	34404.0
68	27636.0
69	20868.0
70	17698.0
71	14528.0
72	11886.5
73	9245.0
74	7737.0
75	4455.0
76	2681.0
77	2022.5
78	1364.0
79	1147.0
80	930.0
81	658.5
82	387.0
83	299.5
84	212.0
85	158.0
86	104.0
87	74.5
88	45.0
89	31.5
90	18.0
91	15.0
92	12.0
93	7.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2794222.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.643629967202614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.69167935195065	14.670985513355264
2	6.190924718165354	2.3084261920056597
3	2.5097396772066505	1.4037197356754196
4	1.4527652096122472	1.083392679889451
5	1.0315749826155425	0.9616151129653823
6	0.7254510292692007	0.8115024329412751
7	0.6031332419949486	0.7871215089271163
8	0.5036494789422509	0.7511883614858984
9	0.4363355848647394	0.732139126716702
>10	5.312267929973659	22.90781589822963
>50	1.4022096657418184	18.612514442170408
>100	1.1317539014881675	32.69624686757232
>500	0.005805837391896438	0.7425393241320641
>1k	0.0025158628698217903	0.7148654824600869
>5k	0.0	0.0
>10k+	1.9352791306321464E-4	0.8159273214733965
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22614	0.8093129321864906	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3764	0.13470654801229107	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008624941039044142	0.0
2	0.0	0.0	0.0	0.028666297810267045	0.0
3	0.0	0.0	0.0	0.04562987479162357	0.0
4	0.0	0.0	0.0	0.06423970607918769	0.0
5	0.0	0.0	0.0	0.10697074176640224	0.0
6	0.0	0.0	0.0	0.17056626137794348	0.0
7	0.0	0.0	0.0	0.21250995804914571	0.0
8	0.0	0.0	0.0	0.32270163215378017	0.0
9	0.0	0.0	0.0	0.37831639719392374	0.0
10	0.0	0.0	0.0	0.4597701972141083	0.0
11	3.578813709146947E-5	0.0	0.0	0.5256919457365951	0.0
12	3.578813709146947E-5	0.0	0.0	0.5843844905666049	0.0
13	3.578813709146947E-5	0.0	0.0	0.608326754280798	0.0
14	7.157627418293893E-5	0.0	0.0	0.6207810259886294	0.0
15	7.157627418293893E-5	0.0	0.0	0.6328774163255461	0.0
16	7.157627418293893E-5	0.0	0.0	0.6661245956835212	0.0
17	7.157627418293893E-5	0.0	0.0	0.7030579531619177	0.0
18	7.157627418293893E-5	0.0	0.0	0.761249464072647	0.0
19	7.157627418293893E-5	0.0	0.0	0.786480100722133	0.0
20	7.157627418293893E-5	0.0	0.0	0.8147527290243939	0.0
21	7.157627418293893E-5	0.0	0.0	0.8488588236725643	0.0
22	7.157627418293893E-5	0.0	0.0	0.8860426981106011	0.0
23	7.157627418293893E-5	0.0	0.0	0.9312789033942185	0.0
24	7.157627418293893E-5	0.0	0.0	0.9684627778322553	0.0
25	7.157627418293893E-5	0.0	0.0	0.997486957013437	0.0
26	7.157627418293893E-5	0.0	0.0	1.0288015769684729	0.0
27	7.157627418293893E-5	0.0	0.0	1.0621203326006308	0.0
28	7.157627418293893E-5	0.0	0.0	1.0991610544903019	0.0
29	1.0736441127440841E-4	0.0	0.0	1.1398521663633026	0.0
30	1.0736441127440841E-4	0.0	0.0	1.1927828211215858	0.0
31	1.4315254836587787E-4	0.0	0.0	1.2397010688485024	0.0
32	1.4315254836587787E-4	0.0	0.0	1.2844720283499307	0.0
33	1.4315254836587787E-4	0.0	0.0	1.3340385982216159	0.0
34	1.4315254836587787E-4	0.0	0.0	1.3834620155449353	0.0
35	1.4315254836587787E-4	0.0	0.0	1.4453039164389945	0.0
36	1.7894068545734734E-4	0.0	0.0	1.4973756559070825	0.0
37	1.7894068545734734E-4	0.0	0.0	1.5528114802617687	0.0
38	1.7894068545734734E-4	0.0	0.0	1.6220973136708536	0.0
39	1.7894068545734734E-4	0.0	0.0	1.7198347160676568	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	595	0.0	43.865547	31
CGTTTTT	17605	0.0	43.287422	1
TCACGAC	625	0.0	41.760002	25
CGACGGT	635	0.0	41.45669	28
CACGACG	650	0.0	40.5	26
ACGACGG	655	0.0	40.190838	27
TACGCGG	140	0.0	40.17857	2
CATATGC	2120	0.0	39.693398	33
ACGGGTA	230	0.0	39.130432	5
TCGTTAG	105	0.0	38.57143	1
ACGTATC	35	6.251501E-6	38.571426	45
CGTAAGG	310	0.0	38.46774	2
ACCCGCT	1550	0.0	38.322582	34
GCCAGTA	2225	0.0	38.224716	25
CTCACGA	685	0.0	38.102192	24
ACTAACC	710	0.0	38.02817	14
ATATGCT	2205	0.0	37.959183	34
ACGGTCT	690	0.0	37.826088	30
GGTACCT	2340	0.0	37.596157	8
CGAAAGG	515	0.0	37.572815	2
>>END_MODULE