FastQCFastQC Report
Thu 26 May 2016
SRR1545059_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545059_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2242113
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC102760.45831766730757995No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92070.41063942807521303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGT302.166429E-645.00000440
ATATCCG453.8562575E-1045.0000041
CACTACG302.166429E-645.0000041
ACTCGGT302.166429E-645.00000440
ACGTATA600.045.00000413
CGGTCTA3000.045.00000431
TACCGTA302.166429E-645.00000438
CTCCGTA253.891997E-545.022
GTCGACA406.8193913E-945.030
ATCGTCC207.034725E-445.025
ATGTACG950.045.01
CCGTATT253.891997E-545.024
TCCGTCA351.2126111E-745.029
CCGAATA207.034725E-445.045
GGTTCAC253.891997E-545.08
TTCGATC406.8193913E-945.014
TGCGTAT502.1827873E-1145.013
CGTACAG551.8189894E-1244.99999611
TGTACGT551.8189894E-1244.99999610
TAGTGCG1900.043.815791