##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545059_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2242113
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93972070096378	31.0	31.0	33.0	28.0	34.0
2	31.220678440381906	31.0	31.0	34.0	28.0	34.0
3	31.299313192510816	31.0	31.0	34.0	28.0	34.0
4	35.11630412918528	35.0	35.0	37.0	32.0	37.0
5	34.970703528323504	35.0	35.0	37.0	32.0	37.0
6	35.035532107436154	37.0	35.0	37.0	32.0	37.0
7	35.58888601957172	37.0	35.0	37.0	35.0	37.0
8	35.536265121338666	37.0	35.0	37.0	35.0	37.0
9	37.280982269849915	39.0	37.0	39.0	35.0	39.0
10	36.66185914804473	39.0	35.0	39.0	32.0	39.0
11	36.26576626601781	38.0	35.0	39.0	32.0	39.0
12	34.87363348769665	35.0	34.0	39.0	30.0	39.0
13	34.126615384684	35.0	33.0	39.0	25.0	39.0
14	34.962916231251505	36.0	33.0	40.0	25.0	41.0
15	35.544670139283795	36.0	33.0	40.0	30.0	41.0
16	35.79567176141435	36.0	34.0	40.0	31.0	41.0
17	35.743851893281025	36.0	34.0	40.0	31.0	41.0
18	35.704790525722835	36.0	34.0	40.0	30.0	41.0
19	35.55622843273287	36.0	34.0	40.0	30.0	41.0
20	35.38266358564444	35.0	34.0	40.0	30.0	41.0
21	35.131515672938875	35.0	34.0	40.0	29.0	41.0
22	35.011369632128265	35.0	34.0	40.0	29.0	41.0
23	35.04443130207978	35.0	34.0	39.0	29.0	41.0
24	34.94464507364258	35.0	34.0	39.0	29.0	41.0
25	34.83181043952736	35.0	34.0	39.0	29.0	41.0
26	34.65866974590487	35.0	34.0	39.0	29.0	41.0
27	34.534140785946114	35.0	33.0	39.0	27.0	41.0
28	34.636247147222285	35.0	34.0	39.0	27.0	41.0
29	34.740623688458165	35.0	34.0	39.0	29.0	41.0
30	34.66303393272328	35.0	34.0	39.0	29.0	41.0
31	34.39864226290111	35.0	33.0	39.0	27.0	41.0
32	34.10129864105868	35.0	33.0	39.0	25.0	41.0
33	33.853115342536256	35.0	33.0	39.0	24.0	41.0
34	33.69295749143777	35.0	33.0	39.0	23.0	41.0
35	33.49426946813118	35.0	33.0	39.0	23.0	41.0
36	33.24222061956735	35.0	33.0	39.0	22.0	41.0
37	33.133530290400174	35.0	32.0	39.0	22.0	41.0
38	33.17201184775254	35.0	32.0	39.0	23.0	41.0
39	33.1840843882534	35.0	32.0	39.0	23.0	41.0
40	32.90216193385436	35.0	32.0	39.0	21.0	41.0
41	32.98081318827374	35.0	32.0	38.0	22.0	41.0
42	32.968634943912285	35.0	32.0	38.0	22.0	41.0
43	32.82729059596907	35.0	32.0	38.0	22.0	40.0
44	32.78256002262152	35.0	32.0	38.0	22.0	40.0
45	32.75810229011651	35.0	32.0	38.0	22.0	40.0
46	32.731120599184784	35.0	32.0	38.0	22.0	40.0
47	32.65317537519295	35.0	32.0	38.0	22.0	40.0
48	32.67662066987703	35.0	32.0	38.0	22.0	40.0
49	32.751185600368935	35.0	32.0	38.0	22.0	40.0
50	32.63418703696023	35.0	32.0	38.0	21.0	40.0
51	32.39261179075274	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	3.0
13	11.0
14	24.0
15	110.0
16	318.0
17	908.0
18	2087.0
19	3732.0
20	6596.0
21	10414.0
22	15200.0
23	21690.0
24	29929.0
25	41502.0
26	52148.0
27	59436.0
28	63702.0
29	69719.0
30	80975.0
31	98420.0
32	121553.0
33	157266.0
34	274383.0
35	372282.0
36	134073.0
37	154148.0
38	203580.0
39	267806.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.32911365305852	23.824579760252938	29.20178420980566	16.64452237688288
2	30.719950332565755	25.371468788593614	30.85290527283861	13.055675606002017
3	28.371317591932254	23.71802848473739	34.512488888829424	13.398165034500936
4	25.563653571430162	27.143814785427857	32.849414815399584	14.443116827742402
5	22.326305587630955	32.07603720240684	31.853925292793008	13.743731917169205
6	20.21994431145977	39.70651791412833	29.202096415301103	10.871441359110804
7	84.99879354876404	5.043991984346909	8.407337185949148	1.549877280939899
8	87.4352898359717	3.265803284669417	7.8925103239667225	1.406396555392168
9	84.68110215676018	4.197469083850814	8.86422762813471	2.257201131254312
10	59.271901104003234	18.52993136385187	13.209013105048676	8.989154427096226
11	55.73113397942031	16.36706089300584	18.037003487335383	9.864801640238472
12	48.64875231533826	19.657840617310544	21.68204724739565	10.011359819955551
13	20.145996209825284	47.2687594247034	21.880743744851397	10.704500620619923
14	12.70069795768545	49.05470866098185	27.724204801452917	10.520388579879784
15	10.563651341390912	20.816702815602962	57.255633413659346	11.364012429346781
16	12.254377901559824	14.558722062625746	55.08803525959664	18.09886477621779
17	13.057682641329851	14.547928672640495	30.687168755544437	41.707219930485216
18	20.600433608832383	21.349280790040467	37.993936969278536	20.056348631848618
19	30.6928776560325	21.363597642045697	27.968973909878763	19.97455079204304
20	33.411830715044246	19.749450629829987	25.383555601345698	21.455163053780073
21	20.84520271725823	26.880491750415793	30.867355927199032	21.40694960512695
22	21.650915899421662	23.171891871640725	26.317719044490623	28.859473184446994
23	17.066713408289413	30.305386035404997	25.180934234804404	27.446966321501193
24	18.32436634549641	21.298569697423815	44.41328336261375	15.963780594466023
25	14.930558807696134	22.107761740822163	41.565166430059506	21.3965130214222
26	13.867186890223643	35.89074234884683	30.20320563682562	20.038865124103914
27	15.205433446039518	37.32238294858466	29.832260907456494	17.639922697919328
28	12.763049855203551	28.91428754928944	43.03659093007355	15.286071665433454
29	13.01682832221213	23.201239188212192	42.88004217450235	20.901890315073327
30	16.479008863514018	30.521744443745696	35.11705253035864	17.882194162381644
31	30.470854947988794	25.38154856601786	26.610969206279968	17.536627279713375
32	32.14548062474996	26.01702055159575	27.785843086410004	14.051655737244287
33	30.723964403221427	26.776170514153392	24.20002916891343	18.299835913711753
34	19.17664274726564	28.523540071352336	28.244829765493535	24.054987415888498
35	19.563286952976945	25.916936389914337	32.0879010112336	22.431875645875117
36	33.69018421462255	23.140582120526485	26.161750099125246	17.00748356572572
37	19.467439865876518	33.93196507044917	29.64382259056524	16.956772473109073
38	20.740078666864694	33.469142723850226	23.44667730841398	22.344101300871095
39	20.81710422266853	32.67828160311278	27.205408469599885	19.299205704618814
40	23.41260230862584	24.939197979762838	26.414279744152058	25.23391996745927
41	16.042991588737944	24.395559010629704	27.90037790245184	31.661071498180508
42	23.473616182592046	24.726942843647933	23.77716912573095	28.022271848029067
43	23.70504965628405	25.52810674573494	25.544564435423194	25.22227916255782
44	18.332349886022694	32.547958109158635	28.503871125139547	20.615820879679127
45	15.506042737364261	41.74963527708015	21.74957283598106	20.99474914957453
46	22.249324632612183	34.514629726512446	25.55723105838109	17.678814582494283
47	23.42272668683514	27.95876033009933	25.440867610151674	23.177645372913855
48	24.396183421620588	24.17326869787562	30.998616037639493	20.431931842864298
49	22.24160869679628	23.275454894557054	33.12295143019107	21.35998497845559
50	19.57055688094222	35.867193134333554	25.10346267114994	19.458787313574295
51	17.954001426333107	36.70912215396815	23.515986928401915	21.82088949129683
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2236.0
1	2612.5
2	2989.0
3	10367.0
4	17745.0
5	14157.0
6	10569.0
7	11847.5
8	13126.0
9	14255.0
10	15384.0
11	15845.0
12	16306.0
13	15856.5
14	15407.0
15	14363.0
16	13319.0
17	12364.0
18	11409.0
19	10850.5
20	10292.0
21	10174.0
22	10056.0
23	9791.5
24	9527.0
25	9781.0
26	11510.5
27	12986.0
28	15388.0
29	17790.0
30	21750.5
31	25711.0
32	27654.0
33	29597.0
34	37615.0
35	45633.0
36	49455.5
37	53278.0
38	70719.0
39	88160.0
40	132005.5
41	175851.0
42	222539.0
43	269227.0
44	262991.5
45	256756.0
46	243816.5
47	230877.0
48	209998.5
49	189120.0
50	175491.0
51	161862.0
52	146556.5
53	131251.0
54	116358.0
55	101465.0
56	90711.0
57	79957.0
58	75523.5
59	71090.0
60	62538.0
61	53986.0
62	46233.0
63	38480.0
64	29724.5
65	20969.0
66	16612.5
67	12256.0
68	9730.5
69	7205.0
70	5536.5
71	3868.0
72	3379.5
73	2891.0
74	2373.0
75	1226.0
76	597.0
77	594.5
78	592.0
79	397.0
80	202.0
81	152.5
82	103.0
83	75.0
84	47.0
85	32.0
86	17.0
87	15.5
88	14.0
89	13.5
90	13.0
91	8.5
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2242113.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.179496670965214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.06568434845079	12.608952790387558
2	6.712195688562415	2.037755042188005
3	2.3228525693622672	1.057791985313326
4	1.152125638018926	0.6995474914736786
5	0.6809033123837156	0.5167884781788898
6	0.4346312435985057	0.3958490109180593
7	0.3179190987676684	0.33780963309660583
8	0.24888399211899442	0.3022346983861447
9	0.19824882588893228	0.2708385653343429
>10	2.2130827344649564	7.901147069949317
>50	0.8169538177969007	8.966037206379363
>100	1.7986584636322849	60.18206877377107
>500	0.03549400026900674	3.4891012391613505
>1k	0.001774700013450337	0.3593220678798148
>5k	2.9578333557505617E-4	0.41337976745838495
>10k+	2.9578333557505617E-4	0.4613761801240756
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10276	0.45831766730757995	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9207	0.41063942807521303	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012934227668275417	0.0
2	0.0	0.0	0.0	0.04089891990278813	0.0
3	0.0	0.0	0.0	0.05534957426320618	0.0
4	0.0	0.0	0.0	0.08478609240479851	0.0
5	0.0	0.0	0.0	0.12934227668275416	0.0
6	0.0	0.0	0.0	0.1874124988348045	0.0
7	0.0	0.0	0.0	0.22764240696164734	0.0
8	0.0	0.0	0.0	0.34900114311812114	0.0
9	0.0	0.0	0.0	0.40359250403525604	0.0
10	0.0	0.0	0.0	0.49127764746915076	0.0
11	0.0	0.0	0.0	0.5615684847284682	0.0
12	0.0	0.0	0.0	0.6360071949986463	0.0
13	0.0	0.0	0.0	0.6666479343369403	0.0
14	0.0	0.0	0.0	0.6773967235371277	0.0
15	0.0	0.0	0.0	0.6938544132253815	0.0
16	0.0	0.0	0.0	0.7323002899497036	0.0
17	0.0	0.0	0.0	0.7758752569562729	0.0
18	0.0	0.0	0.0	0.8624007799785292	0.0
19	0.0	0.0	0.0	0.8913020886993653	0.0
20	0.0	0.0	0.0	0.9276071277406625	0.0
21	0.0	0.0	0.0	0.9670788225214341	0.0
22	0.0	0.0	0.0	1.0032500592075422	0.0
23	0.0	0.0	0.0	1.0490104646821994	0.0
24	0.0	0.0	0.0	1.0838882786014798	0.0
25	0.0	0.0	0.0	1.1069468844790606	0.0
26	0.0	0.0	0.0	1.1356251892745817	0.0
27	0.0	0.0	0.0	1.165240110556426	0.0
28	0.0	0.0	0.0	1.1967728655959802	0.0
29	0.0	0.0	0.0	1.2329441022820884	0.0
30	0.0	0.0	0.0	1.2813805548605266	0.0
31	0.0	0.0	0.0	1.332047046692116	0.0
32	0.0	0.0	0.0	1.373347373660471	0.0
33	0.0	0.0	0.0	1.4150045069093307	0.0
34	0.0	0.0	0.0	1.461656928085248	0.0
35	0.0	0.0	0.0	1.5254360507253648	0.0
36	0.0	0.0	0.0	1.5769499574731514	0.0
37	0.0	4.4600785063018676E-5	0.0	1.628107057940434	0.0
38	0.0	4.4600785063018676E-5	0.0	1.676231305023431	0.0
39	0.0	4.4600785063018676E-5	0.0	1.725961180368697	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGT	30	2.166429E-6	45.000004	40
ATATCCG	45	3.8562575E-10	45.000004	1
CACTACG	30	2.166429E-6	45.000004	1
ACTCGGT	30	2.166429E-6	45.000004	40
ACGTATA	60	0.0	45.000004	13
CGGTCTA	300	0.0	45.000004	31
TACCGTA	30	2.166429E-6	45.000004	38
CTCCGTA	25	3.891997E-5	45.0	22
GTCGACA	40	6.8193913E-9	45.0	30
ATCGTCC	20	7.034725E-4	45.0	25
ATGTACG	95	0.0	45.0	1
CCGTATT	25	3.891997E-5	45.0	24
TCCGTCA	35	1.2126111E-7	45.0	29
CCGAATA	20	7.034725E-4	45.0	45
GGTTCAC	25	3.891997E-5	45.0	8
TTCGATC	40	6.8193913E-9	45.0	14
TGCGTAT	50	2.1827873E-11	45.0	13
CGTACAG	55	1.8189894E-12	44.999996	11
TGTACGT	55	1.8189894E-12	44.999996	10
TAGTGCG	190	0.0	43.81579	1
>>END_MODULE