FastQCFastQC Report
Thu 26 May 2016
SRR1545058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545058_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences563864
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24020.42598924563369894No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21690.3846672247208547No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG207.030225E-445.0000041
CAGCGCG406.8048394E-945.0000041
AGTCCGG207.030225E-445.0000042
ACACGAA207.030225E-445.00000425
AATCATC207.030225E-445.00000419
CGTATGT207.030225E-445.00000440
TACCATC207.030225E-445.00000412
GTACAGT207.030225E-445.0000048
GGTCGCG207.030225E-445.00000428
CGACGGT207.030225E-445.00000428
TTAGCGA207.030225E-445.0000042
TCCGACA207.030225E-445.00000417
TTAGACT207.030225E-445.00000417
GGCGTAA207.030225E-445.00000445
GACCGAC207.030225E-445.0000049
GGTCCGT207.030225E-445.0000049
CCAATCG207.030225E-445.00000432
CGACCTT207.030225E-445.00000420
TAAACGG207.030225E-445.0000042
GGATCGA406.8048394E-945.0000048