##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545058_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	563864
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98539186754253	31.0	31.0	33.0	30.0	34.0
2	31.26197274520097	31.0	31.0	34.0	28.0	34.0
3	31.36128037966602	31.0	31.0	34.0	28.0	34.0
4	35.15183803186584	37.0	35.0	37.0	32.0	37.0
5	35.00356291588042	35.0	35.0	37.0	32.0	37.0
6	35.05508775165643	37.0	35.0	37.0	32.0	37.0
7	35.61318509427805	37.0	35.0	37.0	35.0	37.0
8	35.57157577004384	37.0	35.0	37.0	35.0	37.0
9	37.33104613878524	39.0	37.0	39.0	35.0	39.0
10	36.74439935871061	39.0	37.0	39.0	32.0	39.0
11	36.31131797738462	38.0	35.0	39.0	32.0	39.0
12	34.74508746790006	35.0	33.0	39.0	30.0	39.0
13	33.87663869301817	35.0	33.0	39.0	24.0	39.0
14	34.68495062639218	35.0	33.0	40.0	25.0	41.0
15	35.33864548898316	35.0	33.0	40.0	30.0	41.0
16	35.65609969779947	35.0	34.0	40.0	31.0	41.0
17	35.62033930167558	35.0	34.0	40.0	31.0	41.0
18	35.563412454066935	36.0	34.0	39.0	30.0	41.0
19	35.45155924123547	36.0	34.0	39.0	30.0	41.0
20	35.2616162762652	35.0	34.0	39.0	30.0	41.0
21	34.9771647063831	35.0	34.0	39.0	29.0	41.0
22	34.847855156562574	35.0	33.0	39.0	29.0	41.0
23	34.92962487408311	35.0	34.0	39.0	29.0	41.0
24	34.8277421505895	35.0	34.0	39.0	29.0	41.0
25	34.74689286778372	35.0	34.0	39.0	29.0	41.0
26	34.585754720996555	35.0	34.0	39.0	29.0	41.0
27	34.42824156179504	35.0	33.0	39.0	27.0	41.0
28	34.55930685413504	35.0	34.0	39.0	29.0	41.0
29	34.69271490997829	35.0	34.0	39.0	29.0	41.0
30	34.641303576749	35.0	34.0	39.0	29.0	41.0
31	34.35895003050381	35.0	33.0	39.0	27.0	41.0
32	34.03499957436545	35.0	33.0	39.0	26.0	41.0
33	33.75029085027595	35.0	33.0	39.0	24.0	41.0
34	33.653950243321084	35.0	33.0	39.0	23.0	41.0
35	33.47586652100507	35.0	33.0	39.0	23.0	41.0
36	33.2063352155839	35.0	33.0	38.0	22.0	41.0
37	33.14681554417377	35.0	32.0	38.0	23.0	41.0
38	33.2425141523488	35.0	32.0	38.0	23.0	41.0
39	33.25645368386703	35.0	32.0	38.0	23.0	41.0
40	32.971661606344796	35.0	32.0	38.0	23.0	41.0
41	33.02702779393613	35.0	32.0	38.0	23.0	41.0
42	33.036776598612434	35.0	32.0	38.0	23.0	40.0
43	32.85826724174623	35.0	32.0	38.0	23.0	40.0
44	32.835240412581754	35.0	32.0	38.0	23.0	40.0
45	32.790761602088445	35.0	32.0	38.0	23.0	40.0
46	32.762967665962	35.0	32.0	38.0	23.0	40.0
47	32.68210596881517	35.0	32.0	38.0	23.0	40.0
48	32.77338507157754	35.0	32.0	38.0	23.0	40.0
49	32.856756239093116	35.0	32.0	38.0	23.0	40.0
50	32.72937445908943	35.0	32.0	37.0	23.0	40.0
51	32.491568888951946	35.0	32.0	37.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	1.0
12	1.0
13	2.0
14	7.0
15	28.0
16	76.0
17	224.0
18	487.0
19	946.0
20	1571.0
21	2535.0
22	3652.0
23	5245.0
24	7276.0
25	10185.0
26	13033.0
27	14561.0
28	15614.0
29	17410.0
30	20490.0
31	24908.0
32	30725.0
33	40243.0
34	72556.0
35	102219.0
36	31535.0
37	35379.0
38	47860.0
39	65069.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.34904870678036	23.491834910545805	29.5594327710228	15.599683611651036
2	29.84443057191096	26.176524835776004	30.709000751954374	13.270043840358667
3	28.432742647163145	24.50519983542131	33.45611707787693	13.60594043953861
4	25.632776697927163	27.052445270490754	31.9906927911695	15.324085240412582
5	23.22687740306173	31.823808577954964	31.04791226253139	13.90140175645191
6	20.455287090504093	40.07455698537236	29.218215739965665	10.251940184157881
7	85.98828795596101	4.857731651603933	7.651490430316531	1.502489962118525
8	88.56373167997957	3.2566363520281487	7.017826993743172	1.1618049742491097
9	85.75649447384475	3.5783451328689186	8.46959550529915	2.195564887987174
10	64.63349318275328	16.675474937218905	11.963700466779223	6.727331413248584
11	61.4204843721181	15.373033213682733	15.547898074713052	7.658584339486117
12	53.86156945646468	19.253933572634534	17.911056566831718	8.973440404069066
13	20.493062156832146	51.74350552615524	17.53756224905296	10.225870067959649
14	11.868996779365236	53.357724557694766	25.100910857937375	9.672367805002624
15	9.563298951520224	20.238745513102447	59.85521331384873	10.342742221528596
16	10.779372330916676	13.478072726756807	58.571925145070445	17.170629797256076
17	12.149206191563923	13.198040662287362	29.004157030773374	45.64859611537534
18	20.078600513599024	20.70836230012911	38.79321964161571	20.41981754465616
19	33.55348098122952	20.21285274463346	26.517741866833138	19.715924407303888
20	37.13200346182767	19.044131208944002	23.613495452804223	20.210369876424103
21	20.82310628094718	29.964494984606215	28.467502802094124	20.744895932352485
22	21.605387114623383	24.236872721081678	24.173736929472355	29.98400323482258
23	17.221883291006343	32.75683498148489	22.978413234396946	27.042868493111815
24	18.163599733269013	21.10562121362598	45.88375920434714	14.847019848757856
25	13.348254188953366	21.54650766851581	44.034022388377345	21.07121575415348
26	12.820999389923813	38.970567370855385	29.464551735879574	18.743881503341232
27	15.316991331242994	39.62338436218663	29.929202786487526	15.130421520082857
28	11.5621852077806	29.295894045372645	45.36359831448718	13.778322432359577
29	12.197090078458634	21.796035923556033	45.051998354213076	20.954875643772258
30	15.77845012272463	32.89303803754097	34.013698338606474	17.314813501127933
31	32.89215129889477	25.873082871046922	24.182781663663576	17.051984166394735
32	34.8412028432388	25.406126299958853	26.250833534327427	13.501837322474925
33	32.73041016982818	27.37575018089468	23.875438048891223	16.01840160038591
34	19.261559524991842	27.250720031780713	27.908857454989146	25.5788629882383
35	18.51581232353901	24.702587858065066	34.08038108480059	22.701218733595336
36	35.35409247619993	22.10426627697459	26.234162847778897	16.30747839904658
37	19.13830285317027	35.42201665649873	29.3636408779422	16.076039612388804
38	19.426847608643218	36.00460394705106	21.943943929741923	22.624604514563796
39	19.762389512364685	34.42922406821503	27.307826000595885	18.500560418824396
40	25.83601719563583	24.981378488429833	24.868407984904163	24.31419633103018
41	14.853227019281245	23.83411602797838	27.62811599960274	33.684540953137635
42	23.564370131804832	23.75005320431877	21.885064483634352	30.800512180242045
43	23.06886057630918	25.723756083027112	22.973270150248997	28.23411319041471
44	18.668686066143607	32.122994197182294	28.615765503738487	20.5925542329356
45	15.13414582239689	44.05370798632294	19.672828909098648	21.13931728218152
46	21.652029567413418	37.224756324220024	24.14411985868933	16.979094249677225
47	21.44914376516323	28.568945703219217	24.11184257196771	25.870067959649845
48	24.635905111870947	21.495076827036307	32.23153100747698	21.637487053615764
49	21.086290311138857	21.549522579912885	35.39984819034377	21.964338918604486
50	18.327823730544953	38.06715803810848	24.35605039513074	19.248967836215826
51	16.651710341500785	39.915653419973616	20.706056779648993	22.726579458876607
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	450.0
1	581.0
2	712.0
3	2347.5
4	3983.0
5	3127.0
6	2271.0
7	2547.5
8	2824.0
9	3202.5
10	3581.0
11	3582.0
12	3583.0
13	3554.0
14	3525.0
15	3306.5
16	3088.0
17	2850.5
18	2613.0
19	2500.0
20	2387.0
21	2192.0
22	1997.0
23	2008.5
24	2020.0
25	2006.5
26	2290.5
27	2588.0
28	3375.0
29	4162.0
30	4068.0
31	3974.0
32	5844.0
33	7714.0
34	9335.5
35	10957.0
36	11868.5
37	12780.0
38	16985.0
39	21190.0
40	32669.0
41	44148.0
42	58250.0
43	72352.0
44	73051.0
45	73750.0
46	68316.5
47	62883.0
48	53751.0
49	44619.0
50	41461.5
51	38304.0
52	35660.5
53	33017.0
54	28985.5
55	24954.0
56	22690.0
57	20426.0
58	18715.5
59	17005.0
60	15235.0
61	13465.0
62	11662.5
63	9860.0
64	7240.0
65	4620.0
66	3576.5
67	2533.0
68	1950.5
69	1368.0
70	1111.5
71	855.0
72	732.0
73	609.0
74	458.0
75	247.5
76	188.0
77	160.0
78	132.0
79	92.5
80	53.0
81	33.0
82	13.0
83	11.0
84	9.0
85	4.5
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	563864.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.942805558331237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.44872881551434	14.255330929933619
2	5.664478153566015	2.032732601977003
3	1.9520715670054354	1.0507692168817646
4	1.0707475227736674	0.7684885841277104
5	0.7428114264950051	0.6664060496053267
6	0.6358378213673507	0.6845228637256394
7	0.4908747570566786	0.6165369223552748
8	0.40589643648678936	0.5826336669361605
9	0.3919000075054977	0.6328607069681728
>10	5.723539650289639	25.544216987171893
>50	2.660321224352687	34.084787032157784
>100	0.8107931277527354	18.260758006334328
>500	0.0	0.0
>1k	0.0019994898341621097	0.8199564318253227
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2402	0.42598924563369894	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2169	0.3846672247208547	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01471986152689301	0.0
2	0.0	0.0	0.0	0.045046323226877405	0.0
3	0.0	0.0	0.0	0.06242640069236553	0.0
4	0.0	0.0	0.0	0.09594512151866408	0.0
5	0.0	0.0	0.0	0.15961295631570732	0.0
6	0.0	0.0	0.0	0.22026587971567613	0.0
7	0.0	0.0	0.0	0.2559127732928508	0.0
8	0.0	0.0	0.0	0.363208149482854	0.0
9	0.0	0.0	0.0	0.40931855908516945	0.0
10	0.0	0.0	0.0	0.4804349985102791	0.0
11	0.0	0.0	0.0	0.5460536583289588	0.0
12	0.0	0.0	0.0	0.6111402749599194	0.0
13	0.0	0.0	0.0	0.6414667366599038	0.0
14	0.0	0.0	0.0	0.6499794276634082	0.0
15	0.0	0.0	0.0	0.6664727664826979	0.0
16	0.0	0.0	0.0	0.7028290509768312	0.0
17	0.0	0.0	0.0	0.7475206787452293	0.0
18	0.0	0.0	0.0	0.8347757615311494	0.0
19	0.0	0.0	0.0	0.8596044436247038	0.0
20	0.0	0.0	0.0	0.8878027325738121	0.0
21	0.0	0.0	0.0	0.9246910602556645	0.0
22	0.0	0.0	0.0	0.9599832583743598	0.0
23	0.0	0.0	0.0	1.0044975384135182	0.0
24	0.0	0.0	0.0	1.0387256501567754	0.0
25	0.0	0.0	0.0	1.064086375438049	0.0
26	0.0	0.0	0.0	1.0910432302824795	0.0
27	0.0	0.0	0.0	1.116935998751472	0.0
28	0.0	0.0	0.0	1.1465530695344976	0.0
29	0.0	0.0	0.0	1.1724458380034901	0.0
30	0.0	0.0	0.0	1.2213938112736404	0.0
31	0.0	0.0	0.0	1.2687456549806335	0.0
32	0.0	0.0	0.0	1.3031511144531305	0.0
33	0.0	0.0	0.0	1.342167614885859	0.0
34	0.0	0.0	0.0	1.3817161585063065	0.0
35	0.0	0.0	0.0	1.4434331682817134	0.0
36	0.0	0.0	0.0	1.4895435778840287	0.0
37	0.0	0.0	0.0	1.5381368556956996	0.0
38	0.0	0.0	0.0	1.582651135734858	0.0
39	0.0	0.0	0.0	1.6321311521927273	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.030225E-4	45.000004	1
CAGCGCG	40	6.8048394E-9	45.000004	1
AGTCCGG	20	7.030225E-4	45.000004	2
ACACGAA	20	7.030225E-4	45.000004	25
AATCATC	20	7.030225E-4	45.000004	19
CGTATGT	20	7.030225E-4	45.000004	40
TACCATC	20	7.030225E-4	45.000004	12
GTACAGT	20	7.030225E-4	45.000004	8
GGTCGCG	20	7.030225E-4	45.000004	28
CGACGGT	20	7.030225E-4	45.000004	28
TTAGCGA	20	7.030225E-4	45.000004	2
TCCGACA	20	7.030225E-4	45.000004	17
TTAGACT	20	7.030225E-4	45.000004	17
GGCGTAA	20	7.030225E-4	45.000004	45
GACCGAC	20	7.030225E-4	45.000004	9
GGTCCGT	20	7.030225E-4	45.000004	9
CCAATCG	20	7.030225E-4	45.000004	32
CGACCTT	20	7.030225E-4	45.000004	20
TAAACGG	20	7.030225E-4	45.000004	2
GGATCGA	40	6.8048394E-9	45.000004	8
>>END_MODULE