FastQCFastQC Report
Thu 26 May 2016
SRR1545057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545057_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3470100
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167020.48131177775856604No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC71940.2073139102619521No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT35370.10192789833146018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC302.1667747E-644.99999630
CGGTCTA9000.044.031
CGTTTTT168100.042.764721
CGACGGT9450.042.1428628
CGCATCG3050.042.04918321
TACGGGA9600.041.7187464
ACGGTCT10050.041.64179230
ACGGGAT10550.041.374415
CACGACG10000.040.50000426
CTATACG1000.040.51
ACATACG14300.040.4370617
TCACGAC10050.040.29850825
AGTACGG2700.040.0000042
CATATGC29450.039.5755533
TACGGGT2050.039.5121964
ATACGAA14650.039.47099319
CTCACGT9600.039.37499645
TAGGGTA19200.039.257815
GTTGATC30000.039.22516
TACGAAA14800.039.22297320
Produced by FastQC (version 0.11.5)