##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545057_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3470100
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.923814587475864	31.0	31.0	33.0	30.0	34.0
2	31.203513155240483	31.0	31.0	34.0	28.0	34.0
3	31.298413878562577	31.0	31.0	34.0	28.0	34.0
4	35.13832973113167	35.0	35.0	37.0	32.0	37.0
5	35.01558859975217	35.0	35.0	37.0	32.0	37.0
6	35.07873288954209	37.0	35.0	37.0	32.0	37.0
7	35.58526353707386	37.0	35.0	37.0	35.0	37.0
8	35.51066626322008	37.0	35.0	37.0	35.0	37.0
9	37.24232327598628	39.0	37.0	39.0	34.0	39.0
10	36.57188063744561	39.0	35.0	39.0	32.0	39.0
11	36.279303478285925	38.0	35.0	39.0	32.0	39.0
12	35.46561626466096	37.0	35.0	39.0	30.0	39.0
13	34.98505374484885	37.0	34.0	39.0	29.0	39.0
14	35.97854442235094	38.0	34.0	40.0	29.0	41.0
15	36.32868591683237	38.0	35.0	40.0	30.0	41.0
16	36.41749488487363	38.0	35.0	40.0	31.0	41.0
17	36.288976398374686	38.0	34.0	40.0	31.0	41.0
18	36.254911674015155	38.0	35.0	40.0	30.0	41.0
19	36.183950894786896	37.0	35.0	40.0	30.0	41.0
20	36.05774041093917	37.0	34.0	40.0	30.0	41.0
21	35.88519552750641	37.0	34.0	40.0	30.0	41.0
22	35.76692141436846	37.0	34.0	40.0	30.0	41.0
23	35.74239560819573	37.0	34.0	40.0	30.0	41.0
24	35.6567822252961	36.0	34.0	40.0	30.0	41.0
25	35.5298665744503	36.0	34.0	40.0	29.0	41.0
26	35.38060113541397	36.0	34.0	40.0	29.0	41.0
27	35.30418892827296	36.0	34.0	40.0	29.0	41.0
28	35.3327497766635	36.0	34.0	40.0	29.0	41.0
29	35.34992305697242	36.0	34.0	40.0	29.0	41.0
30	35.23129938618484	36.0	34.0	40.0	29.0	41.0
31	35.06310480965966	36.0	34.0	40.0	27.0	41.0
32	34.855079104348576	36.0	34.0	40.0	27.0	41.0
33	34.60594709086193	36.0	33.0	40.0	25.0	41.0
34	34.41787383648886	36.0	33.0	40.0	24.0	41.0
35	34.26453790957033	36.0	33.0	40.0	24.0	41.0
36	34.0939163136509	36.0	33.0	40.0	23.0	41.0
37	34.05932941413792	36.0	33.0	40.0	23.0	41.0
38	34.05782225296101	35.0	33.0	40.0	23.0	41.0
39	34.009050747817064	36.0	33.0	40.0	23.0	41.0
40	33.80268724244258	35.0	33.0	40.0	23.0	41.0
41	33.77596726319126	35.0	33.0	40.0	23.0	41.0
42	33.74933517766058	35.0	33.0	40.0	23.0	41.0
43	33.630925621740005	35.0	32.0	40.0	23.0	41.0
44	33.555679663410274	35.0	32.0	40.0	23.0	41.0
45	33.499632575430105	35.0	32.0	39.0	23.0	41.0
46	33.42430391055013	35.0	32.0	39.0	23.0	41.0
47	33.353386069565715	35.0	32.0	39.0	23.0	41.0
48	33.34978646148526	35.0	32.0	39.0	23.0	41.0
49	33.35803319789055	35.0	32.0	39.0	23.0	41.0
50	33.27588081035129	35.0	32.0	39.0	23.0	41.0
51	33.10198466902971	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	6.0
12	3.0
13	9.0
14	47.0
15	141.0
16	419.0
17	1116.0
18	2514.0
19	4858.0
20	8185.0
21	13037.0
22	19858.0
23	27906.0
24	38988.0
25	53700.0
26	69749.0
27	80988.0
28	88571.0
29	99403.0
30	115535.0
31	139917.0
32	173234.0
33	218372.0
34	349817.0
35	442393.0
36	258639.0
37	314087.0
38	417839.0
39	530583.0
40	185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.42736520561367	23.145183135932683	27.627503530157632	14.799948128296014
2	32.91951240598254	25.315091784098442	29.445549119621912	12.319846690297108
3	29.521224172214055	24.118094579406936	32.71946053427855	13.641220714100458
4	26.30195095242212	27.465231549523068	31.517276159188494	14.715541338866315
5	22.719546987118527	32.109132301662775	30.869917293449756	14.30140341776894
6	20.522319241520417	40.864787758277856	28.37212184086914	10.240771159332585
7	86.13547160024207	4.149909224518026	8.105069018183913	1.6095501570559927
8	87.06460908907523	3.43759545834414	7.91498804069047	1.5828074118901474
9	83.04616581654707	4.789227976139016	9.386386559465146	2.7782196478487653
10	47.912509725944496	24.885478804645402	15.947436673294717	11.254574796115385
11	43.24221203999885	21.54188640096827	21.53289530561079	13.683006253422093
12	39.31284401025907	20.84415434713697	25.743897870378373	14.099103772225586
13	22.81208034350595	35.45710498256535	25.096423734186335	16.63439093974237
14	16.905045964093254	37.42866199821331	29.35537880752716	16.31091323016628
15	16.830321892740844	22.370335148842972	45.38508976686551	15.414253191550674
16	19.396126912769084	17.959799429411255	43.34987464338204	19.294199014437623
17	17.889484452897612	18.716377049652746	30.596639866286274	32.79749863116337
18	22.393014610529956	23.07970951845768	33.876026627474715	20.65124924353765
19	28.377136105587734	24.751188726549668	26.571338001786692	20.300337166075906
20	31.70090199129708	22.352093599608082	26.19656494049163	19.750439468603208
21	23.3024984870753	27.164289213567333	29.15140774041094	20.381804558946428
22	22.985130111524164	23.470361084694964	27.527477594305637	26.017031209475235
23	21.484741073744274	27.468833751188726	26.981239733725253	24.06518544134175
24	21.161234546554855	23.05204460966543	37.62568802051814	18.16103282326158
25	20.455865825192358	23.699979827670674	34.307166940433994	21.536987406702977
26	18.176911328203797	30.57254834154635	28.473098757960862	22.777441572288986
27	18.285121466240167	31.54848563441976	29.13699893374831	21.02939396559177
28	16.9839197717645	28.76649087922538	35.942854672776	18.306734676234115
29	18.594997262326736	24.146018846719112	35.90599694533299	21.352986945621165
30	20.20215555747673	27.49961096222011	33.29927091438287	18.99896256592029
31	28.768335206478202	26.421659318175266	26.02553240540618	18.784473069940347
32	30.529149015878502	25.065934699288206	26.741678914152327	17.66323737068096
33	28.45926630356474	26.225958906083395	25.13679721045503	20.177977579896833
34	20.538111293622663	27.065415982248346	29.21333679144693	23.183135932682056
35	20.669778968905796	25.71724157805251	31.415953430736863	22.197026022304833
36	28.643036223739948	27.95247975562664	25.55450851560474	17.849975505028674
37	21.867294890637158	32.299904901876026	27.723869629117313	18.1089305783695
38	21.990893634189216	31.897985648828563	24.6459468026858	21.465173914296418
39	22.432869369758794	30.112561597648487	26.13996714792081	21.31460188467191
40	24.130976052563327	25.155643929569756	27.325725483415464	23.387654534451457
41	18.429036627186537	26.30120169447566	28.170830811792168	27.098930866545633
42	22.33560992478603	26.069767441860463	24.624506498371804	26.9701161349817
43	22.1283248321374	26.333448603786636	26.32137402380335	25.216852540272615
44	20.39944093830149	28.8015330970289	28.237514769026827	22.561511195642776
45	19.73176565516844	34.296014524077115	24.16985101293911	21.802368807815338
46	21.57372986369269	31.89472925852281	26.281576899801156	20.24996397798334
47	21.8478430016426	28.383043716319417	26.51309760525633	23.25601567678165
48	22.504884585458633	25.17051381804559	30.4766721420132	21.84792945448258
49	21.857266361199965	25.383792974265873	30.997752226160628	21.761188438373534
50	20.426788853347166	31.510302296763783	27.286764070199705	20.776144779689346
51	18.641018990807183	31.697213336791446	27.127806115097545	22.533961557303826
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1875.0
1	3988.5
2	6102.0
3	21280.0
4	36458.0
5	27612.5
6	18767.0
7	19100.0
8	19433.0
9	19846.5
10	20260.0
11	20204.5
12	20149.0
13	19335.5
14	18522.0
15	17612.5
16	16703.0
17	16281.0
18	15859.0
19	14578.5
20	13298.0
21	14215.5
22	15133.0
23	16722.5
24	18312.0
25	17815.5
26	22859.5
27	28400.0
28	31923.5
29	35447.0
30	40462.0
31	45477.0
32	54689.5
33	63902.0
34	79016.5
35	94131.0
36	100941.5
37	107752.0
38	118766.0
39	129780.0
40	169714.0
41	209648.0
42	247602.0
43	285556.0
44	289205.5
45	292855.0
46	284636.5
47	276418.0
48	268259.5
49	260101.0
50	248498.5
51	236896.0
52	230490.0
53	224084.0
54	208456.0
55	192828.0
56	188724.0
57	184620.0
58	179202.5
59	173785.0
60	159866.0
61	145947.0
62	120136.0
63	94325.0
64	77770.0
65	61215.0
66	48704.0
67	36193.0
68	28342.5
69	20492.0
70	16894.5
71	13297.0
72	11043.0
73	8789.0
74	7122.5
75	3854.5
76	2253.0
77	1661.0
78	1069.0
79	825.5
80	582.0
81	469.5
82	357.0
83	253.0
84	149.0
85	98.5
86	48.0
87	43.5
88	39.0
89	26.0
90	13.0
91	7.0
92	1.0
93	2.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3470100.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.311872478275237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.80895880553416	12.373364723325357
2	6.347250839186391	1.9437659087449501
3	2.2957275966747748	1.054556646154243
4	1.2702913225265204	0.7780215496314272
5	0.8330005834654878	0.6377399354176208
6	0.6028761005608997	0.5538697183193001
7	0.4562382680550257	0.4890103526117396
8	0.36981231223015515	0.4530015172611393
9	0.29779696655223803	0.4103846258638558
>10	3.758537747759195	13.792933027551141
>50	1.2377388122549344	13.562692701028526
>100	1.6982497197110387	50.35021077253901
>500	0.019347858063704575	1.9866306256182564
>1k	0.003793697659549917	0.9197751264923824
>5k	1.8968488297749586E-4	0.2089447473786219
>10k+	1.8968488297749586E-4	0.48509802206251634
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16702	0.48131177775856604	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7194	0.2073139102619521	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3537	0.10192789833146018	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.88176133252644E-5	0.0	0.0	0.005100717558571799	0.0
2	2.88176133252644E-5	0.0	0.0	0.02414915996657157	0.0
3	2.88176133252644E-5	0.0	0.0	0.03645428085645947	0.0
4	2.88176133252644E-5	0.0	0.0	0.054177113051497076	0.0
5	2.88176133252644E-5	0.0	0.0	0.09541511771995044	0.0
6	2.88176133252644E-5	0.0	0.0	0.140053600760785	0.0
7	2.88176133252644E-5	0.0	0.0	0.1697645600991326	0.0
8	2.88176133252644E-5	0.0	0.0	0.254603613728711	0.0
9	2.88176133252644E-5	0.0	0.0	0.29169188207832625	0.0
10	2.88176133252644E-5	0.0	0.0	0.3519783291547794	0.0
11	2.88176133252644E-5	0.0	0.0	0.40716405867266076	0.0
12	2.88176133252644E-5	0.0	0.0	0.4577101524451745	0.0
13	8.64528399757932E-5	0.0	0.0	0.47765194086625745	0.0
14	8.64528399757932E-5	0.0	0.0	0.4864413129304631	0.0
15	8.64528399757932E-5	0.0	0.0	0.49886170427365206	0.0
16	8.64528399757932E-5	0.0	0.0	0.5269876948791101	0.0
17	8.64528399757932E-5	0.0	0.0	0.5595804155499842	0.0
18	8.64528399757932E-5	0.0	0.0	0.6158612143742255	0.0
19	8.64528399757932E-5	0.0	0.0	0.638367770381257	0.0
20	8.64528399757932E-5	0.0	0.0	0.6645053456672718	0.0
21	8.64528399757932E-5	0.0	0.0	0.6969539782715195	0.0
22	8.64528399757932E-5	0.0	0.0	0.7304112273421515	0.0
23	1.152704533010576E-4	0.0	0.0	0.7735800121033976	0.0
24	1.152704533010576E-4	0.0	0.0	0.8060862799342958	0.0
25	1.152704533010576E-4	0.0	0.0	0.832367943286937	0.0
26	1.152704533010576E-4	0.0	0.0	0.8584478833463013	0.0
27	1.152704533010576E-4	0.0	0.0	0.8878418489380709	0.0
28	1.152704533010576E-4	0.0	0.0	0.9170052736232385	0.0
29	1.152704533010576E-4	0.0	0.0	0.9516440448402064	0.0
30	1.152704533010576E-4	0.0	0.0	0.9979827670672315	0.0
31	1.152704533010576E-4	0.0	0.0	1.0379816143626985	0.0
32	1.152704533010576E-4	0.0	0.0	1.075415694072217	0.0
33	1.44088066626322E-4	0.0	0.0	1.119996541886401	0.0
34	1.44088066626322E-4	0.0	0.0	1.162733062447768	0.0
35	1.44088066626322E-4	0.0	0.0	1.22126163511138	0.0
36	1.44088066626322E-4	0.0	0.0	1.266159476672142	0.0
37	1.44088066626322E-4	0.0	0.0	1.3116913057260597	0.0
38	1.44088066626322E-4	0.0	0.0	1.36676176479064	0.0
39	1.44088066626322E-4	0.0	0.0	1.421486412495317	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAC	30	2.1667747E-6	44.999996	30
CGGTCTA	900	0.0	44.0	31
CGTTTTT	16810	0.0	42.76472	1
CGACGGT	945	0.0	42.14286	28
CGCATCG	305	0.0	42.049183	21
TACGGGA	960	0.0	41.718746	4
ACGGTCT	1005	0.0	41.641792	30
ACGGGAT	1055	0.0	41.37441	5
CACGACG	1000	0.0	40.500004	26
CTATACG	100	0.0	40.5	1
ACATACG	1430	0.0	40.43706	17
TCACGAC	1005	0.0	40.298508	25
AGTACGG	270	0.0	40.000004	2
CATATGC	2945	0.0	39.57555	33
TACGGGT	205	0.0	39.512196	4
ATACGAA	1465	0.0	39.470993	19
CTCACGT	960	0.0	39.374996	45
TAGGGTA	1920	0.0	39.25781	5
GTTGATC	3000	0.0	39.225	16
TACGAAA	1480	0.0	39.222973	20
>>END_MODULE