FastQCFastQC Report
Thu 26 May 2016
SRR1545056_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545056_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5066703
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308500.6088772126568303No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC87470.17263691990629804No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG56670.11184788214347674No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA207.035567E-445.012
CGTTTTT250000.043.0921
AGTACGG5050.041.4356462
CGGTCTA10500.039.8571431
TCACGAC10800.039.58333225
ACTCGAC403.4613367E-739.37510
CGCATCG4300.039.24418321
TCGTAAG1950.039.230771
AACGTAG2200.038.8636361
GCGTAAG3150.038.571431
ACGATAT700.038.57142635
TACGATA700.038.57142634
GCGCGAC28300.038.4805649
CGAGGGA16900.038.476334
GCGATAT5750.038.3478249
CACGTTA1350.038.33333225
CGTAAGG6050.038.3057822
GACGGTC11050.038.07692329
TAGGGCG18750.038.045
CGACGGT11200.037.56696328
Produced by FastQC (version 0.11.5)