##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545056_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5066703
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95158212352293	31.0	31.0	33.0	30.0	34.0
2	31.23191925794743	31.0	31.0	34.0	28.0	34.0
3	31.308203184595584	31.0	31.0	34.0	28.0	34.0
4	35.14418903180234	35.0	35.0	37.0	32.0	37.0
5	35.00731955277426	35.0	35.0	37.0	32.0	37.0
6	35.06747188457662	37.0	35.0	37.0	32.0	37.0
7	35.59716249403211	37.0	35.0	37.0	35.0	37.0
8	35.52111738146088	37.0	35.0	37.0	35.0	37.0
9	37.21921336221997	39.0	37.0	39.0	34.0	39.0
10	36.602660349343545	39.0	35.0	39.0	32.0	39.0
11	36.313732026526914	38.0	35.0	39.0	32.0	39.0
12	35.567910532746836	37.0	35.0	39.0	30.0	39.0
13	35.09055750850208	37.0	34.0	39.0	30.0	39.0
14	36.09857949044971	38.0	35.0	40.0	30.0	41.0
15	36.44341359657355	38.0	35.0	40.0	31.0	41.0
16	36.53606734004342	38.0	35.0	40.0	31.0	41.0
17	36.4270181220411	38.0	35.0	40.0	31.0	41.0
18	36.363779957104256	38.0	35.0	40.0	31.0	41.0
19	36.27039615308022	37.0	35.0	40.0	31.0	41.0
20	36.12617652939199	37.0	34.0	40.0	30.0	41.0
21	35.95310224420101	37.0	34.0	40.0	30.0	41.0
22	35.85058488725311	37.0	34.0	40.0	30.0	41.0
23	35.82186009324012	37.0	34.0	40.0	30.0	41.0
24	35.724604935398816	37.0	34.0	40.0	30.0	41.0
25	35.580676428044036	36.0	34.0	40.0	29.0	41.0
26	35.44081900991631	36.0	34.0	40.0	29.0	41.0
27	35.346057189458314	36.0	34.0	40.0	29.0	41.0
28	35.350033739889625	36.0	34.0	40.0	29.0	41.0
29	35.37723407115041	36.0	34.0	40.0	29.0	41.0
30	35.278378464259696	36.0	34.0	40.0	29.0	41.0
31	35.103843465859356	36.0	34.0	40.0	28.0	41.0
32	34.88346386200257	36.0	34.0	40.0	27.0	41.0
33	34.650523624534536	36.0	33.0	40.0	25.0	41.0
34	34.498912803848974	36.0	33.0	40.0	25.0	41.0
35	34.322363477788215	36.0	33.0	40.0	24.0	41.0
36	34.14460922615752	36.0	33.0	40.0	23.0	41.0
37	34.099119091843356	36.0	33.0	40.0	23.0	41.0
38	34.064949534243475	36.0	33.0	40.0	23.0	41.0
39	34.01579172886194	36.0	33.0	40.0	23.0	41.0
40	33.84504144016336	35.0	33.0	40.0	23.0	41.0
41	33.809558405140386	35.0	33.0	40.0	23.0	41.0
42	33.76646252997265	35.0	33.0	40.0	23.0	41.0
43	33.647178056420515	35.0	33.0	40.0	23.0	41.0
44	33.57215293653486	35.0	32.0	40.0	23.0	41.0
45	33.51347335732921	35.0	32.0	39.0	23.0	41.0
46	33.460429790339006	35.0	32.0	39.0	23.0	41.0
47	33.381724367897625	35.0	32.0	39.0	23.0	41.0
48	33.33520713568567	35.0	32.0	39.0	23.0	41.0
49	33.35168471489251	35.0	32.0	39.0	23.0	41.0
50	33.24559185726892	35.0	32.0	39.0	22.0	41.0
51	33.07788259939452	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	5.0
12	13.0
13	13.0
14	50.0
15	170.0
16	574.0
17	1485.0
18	3413.0
19	6843.0
20	11834.0
21	18939.0
22	27799.0
23	40092.0
24	56083.0
25	78369.0
26	101303.0
27	118360.0
28	128804.0
29	142748.0
30	167144.0
31	202137.0
32	247535.0
33	315350.0
34	492667.0
35	656102.0
36	385351.0
37	463947.0
38	609016.0
39	790227.0
40	328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.26650486519537	23.180715348817564	27.963865259124127	15.588914526862933
2	33.08405880510462	25.287608134915345	29.013739309369424	12.614593750610602
3	29.83022687534675	24.798927428744097	32.43509635358536	12.935749342323795
4	27.281231996428446	26.955536963583615	30.91742302637435	14.845808013613587
5	23.135103833794876	32.048158338864546	30.83940384901187	13.977333978328707
6	21.13778920927475	40.18035002248997	28.303711506279328	10.37814926195595
7	84.90807533024928	4.558684414697289	8.793232995894963	1.7400072591584703
8	85.69847097806996	4.131345373904884	8.388571424060183	1.7816122239649728
9	81.00249807419144	5.559670657624889	10.2373081666717	3.200523101511969
10	47.02786012916091	24.542054270795035	16.02900347622507	12.40108212381898
11	41.58199128703616	22.227037977161874	22.174913350950312	14.01605738485165
12	37.65124973774859	20.81783755629647	26.98851699024	14.542395715714934
13	21.472207863772557	35.68438884221159	26.91397147217826	15.929431821837595
14	16.106884496683545	38.61126259028801	29.911147347693362	15.370705565335092
15	15.51582952464354	23.100347504086976	45.97103876031415	15.41278421095533
16	17.761648946070057	18.18509985684971	44.68908084803866	19.364170349041576
17	17.67849822655877	18.439367770323226	30.886416669775198	32.9957173333428
18	21.49034589159854	22.848467731382716	35.100893026490795	20.560293350527946
19	29.13810025967577	22.827487618674315	27.48144108703431	20.55297103461561
20	30.308407656813515	22.635232418399102	26.876965158605117	20.17939476618227
21	23.548844287892937	26.732788560924135	29.990646777598766	19.727720373584162
22	22.798731245940406	23.248550388684713	27.5879995334244	26.36471883195048
23	20.97186276756305	28.786925146392043	26.336633507036034	23.904578579008877
24	20.098316400231077	23.78517154054619	37.35965972349277	18.756852335729963
25	19.759062254093045	23.888078697330396	35.36698322360714	20.985875824969412
26	18.296908265592045	31.23723652244862	29.020449787564022	21.44540542439531
27	19.411202906505473	31.696706911772804	29.26932958178129	19.622760599940435
28	17.503729742990657	28.221073940982922	36.99907020403604	17.27612611199038
29	18.01522607502354	25.419271664433456	35.65059961083174	20.914902649711262
30	20.053356196327275	28.48783518591873	32.33737994905168	19.121428668702308
31	28.764721358248153	26.122371885622663	25.98488602943571	19.128020726693475
32	29.076936224602072	25.903728716682227	27.795155942631727	17.22417911608397
33	27.280047794394108	26.463836542224794	25.85089356925006	20.405222094131034
34	20.517898917698552	28.238047503475137	28.500506147686178	22.743547431140133
35	21.200394023490226	24.51308079435483	31.60240495643814	22.684120225716804
36	29.26546118846911	25.923129893344843	26.185114856742146	18.6262940614439
37	21.311649804616533	31.691160898911974	28.52726911366228	18.469920182809215
38	21.748482198384238	32.300531529083116	24.259404981898484	21.691581290634165
39	22.09417050890885	29.928594590999314	26.430007837443796	21.547227062648037
40	23.9519466603825	25.497961889615397	27.738353718384516	22.811737731617583
41	18.71822761270988	25.379344319175605	28.22863309730213	27.67379497081238
42	22.192202700651688	26.475007514748743	25.173451058804908	26.15933872579466
43	22.692745163866917	25.86030797542307	26.7707817095259	24.67616515118411
44	20.699930507077287	28.531867764895637	29.102218938035247	21.665982789991837
45	19.461551229665524	34.50993673795366	24.623882631367973	21.404629401012848
46	21.97758581862801	31.33258452291362	27.20441675780088	19.48541290065749
47	22.390832855211762	28.101410325412797	27.03511534029131	22.47264147908413
48	22.626291693039832	26.512388825632765	30.14421804475218	20.717101436575224
49	21.84550781839788	25.54675101343023	31.5529053114027	21.054835856769184
50	20.27225594237515	31.39329461387415	28.186436031478458	20.148013412272242
51	19.70133240491894	32.251387144657976	26.073720918711835	21.973559531711253
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3882.0
1	6425.5
2	8969.0
3	32997.5
4	57026.0
5	42556.5
6	28087.0
7	28826.0
8	29565.0
9	30973.0
10	32381.0
11	31971.0
12	31561.0
13	30551.5
14	29542.0
15	27481.0
16	25420.0
17	24360.5
18	23301.0
19	23680.0
20	24059.0
21	23628.0
22	23197.0
23	23825.0
24	24453.0
25	27830.0
26	37561.5
27	43916.0
28	51754.0
29	59592.0
30	67512.5
31	75433.0
32	88165.5
33	100898.0
34	108927.5
35	116957.0
36	126441.0
37	135925.0
38	158845.5
39	181766.0
40	247285.5
41	312805.0
42	384781.5
43	456758.0
44	454278.5
45	451799.0
46	440210.5
47	428622.0
48	409845.5
49	391069.0
50	371113.5
51	351158.0
52	330440.0
53	309722.0
54	291823.5
55	273925.0
56	264221.5
57	254518.0
58	237425.5
59	220333.0
60	205291.5
61	190250.0
62	160475.0
63	130700.0
64	109650.5
65	88601.0
66	69265.5
67	49930.0
68	38362.5
69	26795.0
70	21995.0
71	17195.0
72	14953.0
73	12711.0
74	9957.0
75	5016.5
76	2830.0
77	2124.0
78	1418.0
79	1056.0
80	694.0
81	506.5
82	319.0
83	210.0
84	101.0
85	82.5
86	64.0
87	48.0
88	32.0
89	19.0
90	6.0
91	5.0
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5066703.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.110948361998878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.09199769461483	12.253769897346567
2	7.359765686696759	2.224260784961719
3	2.4668381365899084	1.1182879109825892
4	1.3184364039597696	0.7969129767526228
5	0.8522935369213493	0.6439481812841947
6	0.5708153532072555	0.5175336795930592
7	0.4227405699251229	0.44716076458623555
8	0.3421179927163365	0.41357818573178157
9	0.27558379446907516	0.3747899238863316
>10	2.735336673748963	9.564864429559616
>50	0.8921221642404032	9.7179477114519
>100	1.6057852747622674	53.69208034673053
>500	0.058638768178996124	5.4625891099846235
>1k	0.007131742075823853	1.8689459173858958
>5k	2.6413859540088346E-4	0.2876590935642698
>10k+	1.3206929770044173E-4	0.6156710861979827
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30850	0.6088772126568303	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8747	0.17263691990629804	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5667	0.11184788214347674	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.015414363147001117	0.0
2	0.0	0.0	0.0	0.06015746334450628	0.0
3	0.0	0.0	0.0	0.08840068186353138	0.0
4	0.0	0.0	0.0	0.1282688170196674	0.0
5	0.0	0.0	0.0	0.21953132046618876	0.0
6	0.0	0.0	0.0	0.32466872441506833	0.0
7	0.0	0.0	0.0	0.3988984552676563	0.0
8	0.0	0.0	0.0	0.6088377392556856	0.0
9	0.0	0.0	0.0	0.7004357666119368	0.0
10	0.0	0.0	0.0	0.8479281299890679	0.0
11	0.0	0.0	0.0	0.9649272909819265	0.0
12	0.0	0.0	0.0	1.0834264412182835	0.0
13	0.0	0.0	0.0	1.1329655596548682	0.0
14	0.0	0.0	0.0	1.1498207019436506	0.0
15	0.0	0.0	0.0	1.1785178645758396	0.0
16	0.0	0.0	0.0	1.244004237074879	0.0
17	0.0	0.0	0.0	1.3178194972154476	0.0
18	0.0	0.0	0.0	1.4460488408339702	0.0
19	0.0	0.0	0.0	1.4945616508407933	0.0
20	0.0	0.0	0.0	1.5510086144776989	0.0
21	0.0	0.0	0.0	1.618212079926532	0.0
22	0.0	0.0	0.0	1.6841918699398801	0.0
23	0.0	0.0	0.0	1.760651847957143	0.0
24	0.0	0.0	0.0	1.822171143641141	0.0
25	0.0	0.0	0.0	1.8718484189817324	3.947340114468916E-5
26	0.0	0.0	0.0	1.9220191118366323	3.947340114468916E-5
27	0.0	0.0	0.0	1.9742818949522007	3.947340114468916E-5
28	0.0	0.0	0.0	2.0306893851879613	3.947340114468916E-5
29	0.0	0.0	0.0	2.090629744826172	3.947340114468916E-5
30	0.0	0.0	0.0	2.1717870575796527	3.947340114468916E-5
31	0.0	0.0	0.0	2.244674692793321	3.947340114468916E-5
32	0.0	0.0	0.0	2.312805783169055	3.947340114468916E-5
33	0.0	0.0	0.0	2.387272354428511	3.947340114468916E-5
34	0.0	0.0	0.0	2.459962622636456	3.947340114468916E-5
35	1.973670057234458E-5	0.0	0.0	2.5506132883652346	3.947340114468916E-5
36	1.973670057234458E-5	0.0	0.0	2.632303492034169	3.947340114468916E-5
37	1.973670057234458E-5	0.0	0.0	2.7133226478836434	3.947340114468916E-5
38	1.973670057234458E-5	0.0	0.0	2.7943812771342627	3.947340114468916E-5
39	1.973670057234458E-5	0.0	0.0	2.8765648983175054	3.947340114468916E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	20	7.035567E-4	45.0	12
CGTTTTT	25000	0.0	43.092	1
AGTACGG	505	0.0	41.435646	2
CGGTCTA	1050	0.0	39.85714	31
TCACGAC	1080	0.0	39.583332	25
ACTCGAC	40	3.4613367E-7	39.375	10
CGCATCG	430	0.0	39.244183	21
TCGTAAG	195	0.0	39.23077	1
AACGTAG	220	0.0	38.863636	1
GCGTAAG	315	0.0	38.57143	1
ACGATAT	70	0.0	38.571426	35
TACGATA	70	0.0	38.571426	34
GCGCGAC	2830	0.0	38.480564	9
CGAGGGA	1690	0.0	38.47633	4
GCGATAT	575	0.0	38.347824	9
CACGTTA	135	0.0	38.333332	25
CGTAAGG	605	0.0	38.305782	2
GACGGTC	1105	0.0	38.076923	29
TAGGGCG	1875	0.0	38.04	5
CGACGGT	1120	0.0	37.566963	28
>>END_MODULE