FastQCFastQC Report
Thu 26 May 2016
SRR1545055_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545055_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2922338
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153070.5237929356563136No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC57440.19655495018030084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT148900.042.975151
TAGCCGT2900.041.8965544
CGACGGT5650.041.017728
CACGACG5600.040.58035726
CGGTCTA5700.039.8684231
GACGGTC5800.039.56896629
TATAGCG1900.039.0789451
GCGCGAC13500.039.09
ACCCGCT13200.038.8636434
ATTAGCG1750.038.5714261
ATTACGA356.251599E-638.57142636
TAGTTCG700.038.5714261
CGTAAAT700.038.57142616
GCGATAC1600.037.968759
TACGGGT4300.037.674424
GTTTTTT182050.037.559742
GGGCGAT45950.037.508167
TATGCGA301.1403528E-437.50000439
TAGGGTA19100.037.2251325
TCACGAC6050.037.19008325
Produced by FastQC (version 0.11.5)