##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545055_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2922338
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.854431280707434	31.0	31.0	33.0	28.0	34.0
2	31.143195961589658	31.0	31.0	34.0	28.0	34.0
3	31.231142325083546	31.0	31.0	34.0	28.0	34.0
4	35.072922776215485	35.0	35.0	37.0	32.0	37.0
5	34.93553825738159	35.0	35.0	37.0	32.0	37.0
6	34.99661811878023	36.0	35.0	37.0	32.0	37.0
7	35.55700606842877	37.0	35.0	37.0	35.0	37.0
8	35.486488900325696	37.0	35.0	37.0	35.0	37.0
9	37.19057138496642	39.0	37.0	39.0	34.0	39.0
10	36.43080814060523	38.0	35.0	39.0	32.0	39.0
11	36.17247799535851	38.0	35.0	39.0	31.0	39.0
12	35.59594270067323	37.0	35.0	39.0	30.0	39.0
13	35.23048805442765	37.0	35.0	39.0	30.0	39.0
14	36.22915145339108	38.0	35.0	40.0	30.0	41.0
15	36.51776420112937	38.0	35.0	40.0	31.0	41.0
16	36.56938006486587	38.0	35.0	40.0	31.0	41.0
17	36.45599892962416	38.0	35.0	40.0	31.0	41.0
18	36.3920275478059	38.0	35.0	40.0	30.0	41.0
19	36.32525327323533	38.0	35.0	40.0	30.0	41.0
20	36.20543722184087	38.0	34.0	40.0	30.0	41.0
21	36.06791822164308	38.0	34.0	40.0	30.0	41.0
22	35.960234237107414	37.0	34.0	40.0	30.0	41.0
23	35.898181866710836	37.0	34.0	40.0	30.0	41.0
24	35.8198966033361	37.0	34.0	40.0	30.0	41.0
25	35.684718537007015	37.0	34.0	40.0	29.0	41.0
26	35.55734962896147	37.0	34.0	40.0	29.0	41.0
27	35.45730507559358	37.0	34.0	40.0	29.0	41.0
28	35.48042663100572	37.0	34.0	40.0	29.0	41.0
29	35.495053618027754	37.0	34.0	40.0	29.0	41.0
30	35.36061776563834	37.0	34.0	40.0	29.0	41.0
31	35.209823778084534	37.0	34.0	40.0	27.0	41.0
32	35.01635779297261	37.0	34.0	40.0	27.0	41.0
33	34.78211418391712	37.0	33.0	40.0	25.0	41.0
34	34.58801925033997	37.0	33.0	40.0	24.0	41.0
35	34.39647022349913	37.0	33.0	40.0	24.0	41.0
36	34.24802264488228	37.0	33.0	40.0	23.0	41.0
37	34.205871120999696	37.0	33.0	40.0	23.0	41.0
38	34.15651372291638	37.0	33.0	40.0	23.0	41.0
39	34.08430886502519	36.0	33.0	40.0	23.0	41.0
40	33.949451774572275	36.0	33.0	40.0	23.0	41.0
41	33.876293912613804	36.0	33.0	40.0	23.0	41.0
42	33.85136421591206	36.0	33.0	40.0	23.0	41.0
43	33.736995173042956	36.0	33.0	40.0	23.0	41.0
44	33.682639722030785	36.0	32.0	40.0	23.0	41.0
45	33.6184476265237	36.0	32.0	40.0	23.0	41.0
46	33.57202691817305	35.0	32.0	40.0	23.0	41.0
47	33.45521496828909	35.0	32.0	40.0	22.0	41.0
48	33.405145811333256	35.0	32.0	39.0	22.0	41.0
49	33.414581407078856	35.0	32.0	39.0	22.0	41.0
50	33.34206207495505	35.0	32.0	39.0	22.0	41.0
51	33.160781538617364	35.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	4.0
13	10.0
14	27.0
15	126.0
16	343.0
17	1005.0
18	2169.0
19	4176.0
20	7120.0
21	11285.0
22	16771.0
23	24298.0
24	34010.0
25	46565.0
26	59754.0
27	68654.0
28	74852.0
29	82715.0
30	96403.0
31	115889.0
32	141800.0
33	177341.0
34	262547.0
35	324851.0
36	231287.0
37	280252.0
38	378133.0
39	479844.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.67996378242353	23.328889402936966	28.52483182985678	15.46631498478273
2	32.64290441420534	25.317126218801523	29.200626347807816	12.839343019185323
3	28.525071364092724	24.991428096270862	33.13720041966398	13.346300119972431
4	25.505263251547223	27.035373731580677	32.555474418085794	14.903888598786313
5	22.463452208471434	31.255282585381977	31.983843073593814	14.29742213255277
6	19.89971728116323	40.52563392735543	29.119526899352504	10.455121892128837
7	85.82326890318642	4.034919985299442	8.502096608948042	1.6397145025660962
8	87.09098673733155	3.3008844288374584	7.938061921653142	1.6700669121778522
9	82.88637385545408	4.66113091641008	9.523333714306832	2.929161513828996
10	42.75497906128586	24.897154264838633	18.45638663289462	13.891480040980886
11	37.27635201677561	24.831590322543114	23.428535645089653	14.463522015591627
12	33.496056924284595	22.69142036273696	27.61816052763233	16.19436218534612
13	22.970409309258546	31.677136594055856	27.37787346980397	17.97458062688163
14	18.418026935967024	34.79744642816813	30.025445379692563	16.759081256172284
15	17.811560469733482	24.177593420062976	40.90830697886418	17.10253913133936
16	19.691698906834183	20.22719480087519	40.19853281858567	19.88257347370496
17	19.830868297917625	20.209777240004406	30.989194268424804	28.970160193653165
18	22.622674037021042	23.469769752848578	33.759099734527624	20.148456475602753
19	27.238396106131464	24.249453690846163	28.158891955687537	20.35325824733484
20	28.14944746295603	24.330758454360858	27.011728280575348	20.508065802107765
21	23.76470483564872	26.564996930539863	30.42454363595176	19.24575459785966
22	22.638517515769905	23.48013816334729	28.801151680606417	25.080192640276383
23	20.621468153238947	27.626338910830984	28.434561642082468	23.317631293847597
24	20.320236741951135	25.42241862508717	35.81584334187216	18.441501291089534
25	20.632726262328312	24.746110819487686	33.717078585707746	20.90408433247626
26	19.16438139599184	29.14748396660482	29.431571570434357	22.256563066968983
27	19.306767389672242	29.377368394757898	30.695662171863763	20.6202020437061
28	17.72905803503907	27.22453733962327	36.1659739564691	18.880430668868556
29	18.861233710816478	25.343338108049103	35.18973506829121	20.60569311284321
30	20.817372939064544	27.335304814159073	33.42166443443572	18.425657812340667
31	26.440781319614636	26.086715499712902	28.35647348116474	19.116029699507724
32	26.908317929000685	26.014307722104697	28.421661012518058	18.655713336376557
33	25.917912301725536	26.41330332083421	27.506879765448076	20.161904611992178
34	20.60268182530563	27.523407627728208	29.462197733458623	22.411712813507542
35	21.3361698749426	25.65911266937637	30.948165475725258	22.056551979955774
36	27.31289125351003	26.38685189735068	27.046358087257534	19.253898761881754
37	21.12137610365399	30.603544148555027	28.63813152345827	19.63694822433271
38	21.945271217771527	30.410411116031067	26.104064622230556	21.54025304396685
39	21.326520067151712	28.68901543900808	27.577713460934362	22.406751032905845
40	23.550629667067945	25.368762956235724	28.382137863587303	22.698469513109025
41	19.724891508100704	25.267645289490815	28.974198056487648	26.033265145920836
42	22.084782800620598	26.515618658758843	26.688904568876016	24.71069397174454
43	21.57344564523337	26.614375202320883	27.68317696310283	24.129002189342916
44	20.96369413804974	28.21364263818901	29.076924024531042	21.745739199230204
45	20.13750633910246	31.929913651329862	26.684490295099334	21.248089714468346
46	22.638277981533964	29.444198446586263	27.437722809613398	20.479800762266375
47	22.113834881522944	28.643880345120927	27.206366956868095	22.03591781648803
48	22.258308244973716	27.398404975742025	29.412853680854166	20.930433098430093
49	22.00570228358253	26.137017689261132	30.592251820289096	21.265028206867242
50	21.17547662180076	29.76941750064503	28.011167770463242	21.043938107090966
51	20.33700413846721	30.334102352294636	27.361619360936345	21.967274148301804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1434.0
1	2562.0
2	3690.0
3	16825.0
4	29960.0
5	22955.5
6	15951.0
7	16271.5
8	16592.0
9	17156.5
10	17721.0
11	17905.5
12	18090.0
13	17331.0
14	16572.0
15	15464.0
16	14356.0
17	13733.0
18	13110.0
19	12867.0
20	12624.0
21	12332.5
22	12041.0
23	12511.0
24	12981.0
25	14218.5
26	18423.0
27	21390.0
28	27403.5
29	33417.0
30	36690.0
31	39963.0
32	53183.5
33	66404.0
34	71163.0
35	75922.0
36	84322.0
37	92722.0
38	105844.5
39	118967.0
40	150788.5
41	182610.0
42	207758.5
43	232907.0
44	237462.0
45	242017.0
46	246710.0
47	251403.0
48	247639.0
49	243875.0
50	236366.0
51	228857.0
52	213895.5
53	198934.0
54	184692.0
55	170450.0
56	156565.5
57	142681.0
58	132126.5
59	121572.0
60	107664.5
61	93757.0
62	79969.5
63	66182.0
64	53928.0
65	41674.0
66	32415.5
67	23157.0
68	18326.0
69	13495.0
70	10958.0
71	8421.0
72	6761.5
73	5102.0
74	4139.5
75	2278.5
76	1380.0
77	1040.0
78	700.0
79	510.0
80	320.0
81	234.0
82	148.0
83	122.0
84	96.0
85	63.0
86	30.0
87	22.0
88	14.0
89	11.5
90	9.0
91	6.5
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2922338.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.442957608858332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.8201352908542	14.72119371502561
2	6.215302056058858	2.292571046922871
3	2.4129800528220824	1.3350746647565521
4	1.3962632036611637	1.030048923037263
5	0.9344897112152124	0.8617377064928213
6	0.6752315979056938	0.747196064180183
7	0.5422279087731104	0.7000200435089665
8	0.4222352481608697	0.6229813426237358
9	0.3486400725272767	0.5786958670532873
>10	4.452986262478455	19.413736619787066
>50	1.5306154245538663	20.320142061964503
>100	1.2414159058920768	35.42393159400758
>500	0.004673290685739315	0.5701249949421159
>1k	0.002430111156584444	0.6567969570443399
>5k	1.8693162742957262E-4	0.19802854030038786
>10k+	1.8693162742957262E-4	0.5277198583527223
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15307	0.5237929356563136	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5744	0.19655495018030084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01618567051449901	0.0
2	0.0	0.0	0.0	0.05834369604063595	0.0
3	0.0	0.0	0.0	0.08127054433812926	0.0
4	0.0	0.0	0.0	0.11100700877174371	0.0
5	0.0	0.0	0.0	0.18054037554861896	0.0
6	0.0	0.0	0.0	0.26465111154151233	0.0
7	3.421917656342285E-5	0.0	0.0	0.3266562594744345	0.0
8	3.421917656342285E-5	0.0	0.0	0.4925850466304719	0.0
9	3.421917656342285E-5	0.0	0.0	0.579262220865622	0.0
10	3.421917656342285E-5	0.0	0.0	0.737560131648016	0.0
11	6.84383531268457E-5	0.0	0.0	0.855376756555881	0.0
12	6.84383531268457E-5	0.0	0.0	0.9715850801652649	0.0
13	6.84383531268457E-5	0.0	0.0	1.0175072151133784	0.0
14	6.84383531268457E-5	0.0	0.0	1.0378334059920515	0.0
15	6.84383531268457E-5	0.0	0.0	1.0670223636006513	0.0
16	6.84383531268457E-5	0.0	0.0	1.1299172101242225	0.0
17	6.84383531268457E-5	0.0	0.0	1.2005113713745639	0.0
18	6.84383531268457E-5	0.0	0.0	1.321031311230939	0.0
19	6.84383531268457E-5	0.0	0.0	1.3690408159494214	0.0
20	6.84383531268457E-5	0.0	0.0	1.4264948133994082	0.0
21	6.84383531268457E-5	0.0	0.0	1.494043467935605	0.0
22	6.84383531268457E-5	0.0	0.0	1.5581017664623324	0.0
23	1.0265752969026855E-4	0.0	0.0	1.630646420776789	0.0
24	1.0265752969026855E-4	0.0	0.0	1.688168856579903	0.0
25	1.0265752969026855E-4	0.0	0.0	1.7338514572920722	0.0
26	1.0265752969026855E-4	0.0	0.0	1.784735372841882	0.0
27	1.0265752969026855E-4	0.0	0.0	1.8410259182887128	0.0
28	1.0265752969026855E-4	0.0	0.0	1.8970427103230358	0.0
29	1.0265752969026855E-4	0.0	0.0	1.9573026802512234	0.0
30	1.0265752969026855E-4	0.0	0.0	2.0301553071547507	0.0
31	1.0265752969026855E-4	0.0	0.0	2.095582372744015	0.0
32	1.0265752969026855E-4	0.0	0.0	2.1608383424504627	0.0
33	1.0265752969026855E-4	0.0	0.0	2.232801270763341	0.0
34	1.0265752969026855E-4	1.0265752969026855E-4	0.0	2.3058592127262485	0.0
35	1.0265752969026855E-4	1.0265752969026855E-4	0.0	2.395958304617741	0.0
36	1.0265752969026855E-4	1.0265752969026855E-4	0.0	2.47072720540882	0.0
37	1.0265752969026855E-4	1.0265752969026855E-4	0.0	2.5475150376171407	0.0
38	1.0265752969026855E-4	1.0265752969026855E-4	0.0	2.6306676366662582	0.0
39	1.368767062536914E-4	1.0265752969026855E-4	0.0	2.740784946847353	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	14890	0.0	42.97515	1
TAGCCGT	290	0.0	41.89655	44
CGACGGT	565	0.0	41.0177	28
CACGACG	560	0.0	40.580357	26
CGGTCTA	570	0.0	39.86842	31
GACGGTC	580	0.0	39.568966	29
TATAGCG	190	0.0	39.078945	1
GCGCGAC	1350	0.0	39.0	9
ACCCGCT	1320	0.0	38.86364	34
ATTAGCG	175	0.0	38.571426	1
ATTACGA	35	6.251599E-6	38.571426	36
TAGTTCG	70	0.0	38.571426	1
CGTAAAT	70	0.0	38.571426	16
GCGATAC	160	0.0	37.96875	9
TACGGGT	430	0.0	37.67442	4
GTTTTTT	18205	0.0	37.55974	2
GGGCGAT	4595	0.0	37.50816	7
TATGCGA	30	1.1403528E-4	37.500004	39
TAGGGTA	1910	0.0	37.225132	5
TCACGAC	605	0.0	37.190083	25
>>END_MODULE