FastQCFastQC Report
Thu 26 May 2016
SRR1545054_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545054_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2927115
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177390.606023336971728No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC150130.5128940953806052No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33320.11383222046281065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGT351.212793E-745.00000431
GCCGAAT207.035078E-445.041
TATCTCG551.8189894E-1245.01
ATCGGTT253.8922895E-545.027
GTACGTA406.8212103E-945.011
CGTTTTT155900.043.57121
CGACGGT7300.043.15068428
CGTAATA1150.043.0434844
GCGTAAG1150.043.043481
CGGTCTA7500.042.331
GCTACGA3300.042.27272810
ACTACGG1550.042.096772
TACTCGC2000.041.62545
TGCGTAT603.6379788E-1241.25000437
ACTCACG556.184564E-1140.9090921
TCACGAC7950.040.7547225
CGTAAGG4650.040.6451572
TAATGCG1850.040.1351361
CTAGACG900.040.01
ATTAACG1800.040.01
Produced by FastQC (version 0.11.5)