##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545054_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2927115
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95294547703114	31.0	31.0	33.0	28.0	34.0
2	31.23274213688222	31.0	31.0	34.0	28.0	34.0
3	31.30656294679232	31.0	31.0	34.0	28.0	34.0
4	35.13356871868717	35.0	35.0	37.0	32.0	37.0
5	34.989287062517185	35.0	35.0	37.0	32.0	37.0
6	35.05249366697243	37.0	35.0	37.0	32.0	37.0
7	35.624848699145744	37.0	35.0	37.0	35.0	37.0
8	35.57739378193204	37.0	35.0	37.0	35.0	37.0
9	37.30734426218307	39.0	37.0	39.0	35.0	39.0
10	36.65060340984211	39.0	35.0	39.0	32.0	39.0
11	36.26254041949155	38.0	35.0	39.0	32.0	39.0
12	35.268402163905414	37.0	35.0	39.0	30.0	39.0
13	34.685250835720495	37.0	33.0	39.0	27.0	39.0
14	35.60559868676154	37.0	33.0	40.0	27.0	41.0
15	36.05291729228267	37.0	34.0	40.0	30.0	41.0
16	36.21465880226776	37.0	34.0	40.0	31.0	41.0
17	36.1328728116251	37.0	34.0	40.0	31.0	41.0
18	36.08886873252332	37.0	35.0	40.0	30.0	41.0
19	35.973475931078895	37.0	34.0	40.0	30.0	41.0
20	35.8269535019977	36.0	34.0	40.0	30.0	41.0
21	35.63572254591979	36.0	34.0	40.0	30.0	41.0
22	35.52361762349617	36.0	34.0	40.0	30.0	41.0
23	35.5209901216727	35.0	34.0	40.0	30.0	41.0
24	35.45896317705317	35.0	34.0	40.0	30.0	41.0
25	35.33308462428022	35.0	34.0	40.0	29.0	41.0
26	35.202771329448964	35.0	34.0	40.0	29.0	41.0
27	35.07117417662101	35.0	34.0	40.0	29.0	41.0
28	35.140785722460514	36.0	34.0	40.0	29.0	41.0
29	35.201114066239285	36.0	34.0	40.0	29.0	41.0
30	35.109182591049546	36.0	34.0	40.0	29.0	41.0
31	34.88896643965133	35.0	34.0	40.0	28.0	41.0
32	34.645437230857006	35.0	34.0	40.0	27.0	41.0
33	34.376660636838665	35.0	33.0	40.0	25.0	41.0
34	34.221893229340154	35.0	33.0	40.0	24.0	41.0
35	34.03501058209192	35.0	33.0	40.0	23.0	41.0
36	33.82878738963109	35.0	33.0	40.0	23.0	41.0
37	33.77630533819136	35.0	33.0	40.0	23.0	41.0
38	33.79483347938157	35.0	33.0	40.0	23.0	41.0
39	33.76041699762394	35.0	33.0	40.0	23.0	41.0
40	33.60834507697853	35.0	33.0	40.0	23.0	41.0
41	33.60869320132622	35.0	33.0	40.0	23.0	41.0
42	33.56073779130646	35.0	33.0	39.0	23.0	41.0
43	33.43303628316619	35.0	32.0	39.0	23.0	41.0
44	33.361371521105255	35.0	32.0	39.0	23.0	41.0
45	33.307300191485474	35.0	32.0	39.0	23.0	41.0
46	33.244405498246564	35.0	32.0	39.0	23.0	41.0
47	33.16196049693982	35.0	32.0	39.0	23.0	41.0
48	33.152976224029466	35.0	32.0	39.0	23.0	41.0
49	33.21694911200961	35.0	32.0	39.0	22.0	41.0
50	33.09862646325819	35.0	32.0	39.0	22.0	41.0
51	32.88955234078606	35.0	32.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	7.0
13	6.0
14	23.0
15	70.0
16	323.0
17	834.0
18	2169.0
19	4124.0
20	7218.0
21	11337.0
22	17198.0
23	24777.0
24	34986.0
25	47902.0
26	60876.0
27	70614.0
28	76048.0
29	84932.0
30	99693.0
31	121277.0
32	150275.0
33	191088.0
34	312999.0
35	426286.0
36	201222.0
37	238066.0
38	316732.0
39	425864.0
40	167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.211243835653878	22.722612538284285	29.719843600268526	16.346300025793315
2	31.24585129043444	24.746789927966613	31.21373775885129	12.793621022747653
3	27.663381862345688	24.85027065899358	33.966823988808095	13.519523489852636
4	24.964376186108165	27.454712233718183	33.09343841974094	14.487473160432712
5	22.1312452705138	31.84794584428695	32.143390334851894	13.877418550347356
6	20.006286052990742	40.46226403813994	29.39235390478338	10.139096004085935
7	85.25691679349804	4.705964746858254	8.496010576967423	1.541107882676287
8	87.05062834907409	3.623055465876811	7.788043858884943	1.5382723261641582
9	83.4348496728007	4.893213966653172	9.450636548273641	2.2212998122724934
10	52.27884794413612	24.428524331978757	13.905500808816873	9.38712691506825
11	45.927235520299	20.216253888214165	22.16322214877106	11.69328844271578
12	41.367728975458775	21.21908432022657	25.047632224904042	12.365554479410616
13	21.35027151307687	41.30838726869289	24.816483124168336	12.5248580940619
14	14.954007615006585	42.606423047949946	29.23766233988074	13.201906997162736
15	13.21393248984068	22.664022424810778	50.504916957482024	13.617128127866518
16	14.741580019917222	18.257396788305208	47.855379785215135	19.145643406562435
17	15.327344501326392	18.57620899759661	30.988635567786027	35.10781093329097
18	21.204838211002986	21.85701620879262	37.18500981341696	19.753135766787434
19	28.15731530875965	23.03862335439503	28.307941437217192	20.49611989962813
20	30.3998305498759	22.311081047379417	27.40883087955205	19.880257523192633
21	21.528399123368914	27.811445740942876	30.388727467147685	20.271427668540525
22	22.345483522171147	24.326580950868003	27.116631905476897	26.21130362148395
23	17.433855519854873	29.869478992113397	27.461203266697755	25.235462221333975
24	19.10324671220639	23.635354265206526	40.76095404519467	16.500444977392416
25	16.46146461618351	24.284218419843427	38.18425309562487	21.070063868348186
26	16.18938784434503	33.600558912102876	29.39221725145749	20.817835992094604
27	16.727152845036837	34.69645025904346	30.152043906713605	18.424352989206096
28	14.325060682617528	28.610047777419062	39.381814517024445	17.68307702293897
29	14.692179842609532	23.881535231789663	39.60773662804502	21.818548297555786
30	17.45192792220326	30.880542787010416	33.541217205337	18.126312085449324
31	28.56225327669053	26.724061063538674	26.509788648549854	18.203897011220946
32	29.67126334291615	26.109804363682326	27.956298266381747	16.26263402701978
33	27.961559419428344	27.327180517335332	26.132147182464642	18.57911288077168
34	19.238670158159145	28.043380598302427	29.179926309694014	23.538022933844417
35	19.2311883885669	25.94284816278144	32.985892252268876	21.840071196382787
36	30.63596749700644	25.166554781756094	26.90246198048249	17.295015740754977
37	19.856514007819985	32.76096087786097	29.481486036592347	17.901039077726704
38	20.26483414556654	33.0128812841313	24.80609747140102	21.916187098901137
39	19.836665112235085	31.648090355179075	28.27931256544413	20.235931967141706
40	23.929022262534954	26.10464570063014	25.926928050315755	24.03940398651915
41	16.835894729110404	24.803671874866552	28.435917276909173	29.92451611911387
42	21.354234459527554	25.686213216768046	25.28978191837355	27.669770405330844
43	21.25246189507416	26.129379952615462	26.974819916538983	25.643338235771402
44	18.991122658317146	29.440387548832213	29.83049863090449	21.737991161946148
45	17.16509259116912	37.8680372995253	24.356508029236977	20.6103620800686
46	21.900847763070463	32.64237995432362	26.13522188229707	19.321550400308837
47	20.949125674939317	28.733616547351232	26.75757529171215	23.559682485997303
48	21.983659678557213	25.33825968573151	31.602311491007356	21.075769144703916
49	21.32973935086254	23.77357910433994	33.092925969768864	21.803755575028656
50	19.847119091665345	32.880088414701845	27.20364591073463	20.069146582898178
51	17.763599995217135	33.06979739436271	25.643953175737888	23.522649434682272
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3991.0
1	4595.0
2	5199.0
3	18631.0
4	32063.0
5	23767.5
6	15472.0
7	16300.0
8	17128.0
9	18382.0
10	19636.0
11	19654.0
12	19672.0
13	18950.5
14	18229.0
15	17221.5
16	16214.0
17	15927.5
18	15641.0
19	15187.5
20	14734.0
21	13932.0
22	13130.0
23	13703.0
24	14276.0
25	15888.0
26	19261.0
27	21022.0
28	27373.0
29	33724.0
30	39506.0
31	45288.0
32	48948.5
33	52609.0
34	61441.5
35	70274.0
36	76214.5
37	82155.0
38	102975.5
39	123796.0
40	169823.0
41	215850.0
42	256740.0
43	297630.0
44	308938.5
45	320247.0
46	300704.0
47	281161.0
48	258124.0
49	235087.0
50	217079.0
51	199071.0
52	184837.5
53	170604.0
54	158058.0
55	145512.0
56	134173.5
57	122835.0
58	109742.5
59	96650.0
60	84721.5
61	72793.0
62	61194.0
63	49595.0
64	40905.0
65	32215.0
66	24894.0
67	17573.0
68	12629.0
69	7685.0
70	6382.5
71	5080.0
72	3918.0
73	2756.0
74	2198.0
75	1240.0
76	840.0
77	537.5
78	235.0
79	201.5
80	168.0
81	113.0
82	58.0
83	53.0
84	48.0
85	33.5
86	19.0
87	12.5
88	6.0
89	4.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2927115.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.722128868959018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.31753371143702	13.598022780285692
2	7.529374833915173	2.518143525508528
3	2.5600254502547126	1.2842722646092242
4	1.2995453945414728	0.869246622343388
5	0.7314408527898536	0.6115624100186606
6	0.5196155743116947	0.5213447157574985
7	0.38193099124179447	0.4470689478216182
8	0.2990004187028325	0.3999938826737175
9	0.22661640820550827	0.34105579036498174
>10	2.593959680271378	10.198545077208205
>50	0.9401225122018828	11.35277914372301
>100	1.575117134916517	53.68585374944369
>500	0.023453937935727108	2.5515691524603556
>1k	0.0018516266791363508	0.4937588716677592
>5k	0.0	0.0
>10k+	4.1147259536363347E-4	1.1267830661136042
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17739	0.606023336971728	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	15013	0.5128940953806052	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3332	0.11383222046281065	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.032625981555217336	0.0
2	0.0	0.0	0.0	0.11458381375518215	0.0
3	0.0	0.0	0.0	0.16299325445020096	0.0
4	0.0	0.0	0.0	0.23231065400573603	0.0
5	0.0	0.0	0.0	0.37320023299392063	0.0
6	0.0	0.0	0.0	0.5181552484272056	0.0
7	0.0	0.0	0.0	0.6187662596105722	0.0
8	0.0	0.0	0.0	0.8981198210524698	0.0
9	0.0	0.0	0.0	1.0307760371560393	0.0
10	3.416333147143177E-5	0.0	0.0	1.2450484521448593	0.0
11	3.416333147143177E-5	0.0	0.0	1.4159675994964325	0.0
12	3.416333147143177E-5	0.0	0.0	1.5855543769206197	0.0
13	3.416333147143177E-5	0.0	0.0	1.6599962761968696	0.0
14	3.416333147143177E-5	0.0	0.0	1.6868144914019436	0.0
15	3.416333147143177E-5	0.0	0.0	1.7296211457356476	0.0
16	3.416333147143177E-5	0.0	0.0	1.8349466966620718	0.0
17	3.416333147143177E-5	0.0	0.0	1.9568073000206687	0.0
18	3.416333147143177E-5	0.0	0.0	2.1651011320019884	0.0
19	3.416333147143177E-5	0.0	0.0	2.239303887957938	0.0
20	3.416333147143177E-5	0.0	0.0	2.322764906742646	0.0
21	3.416333147143177E-5	0.0	0.0	2.4192421548179692	0.0
22	3.416333147143177E-5	0.0	0.0	2.5133621330217637	0.0
23	3.416333147143177E-5	0.0	0.0	2.629073336715503	0.0
24	3.416333147143177E-5	0.0	0.0	2.7113044755672395	0.0
25	3.416333147143177E-5	0.0	0.0	2.780109425150703	0.0
26	3.416333147143177E-5	0.0	0.0	2.8499392746783094	0.0
27	3.416333147143177E-5	0.0	0.0	2.922536354055102	0.0
28	3.416333147143177E-5	0.0	0.0	3.003332632985038	0.0
29	3.416333147143177E-5	0.0	0.0	3.0874427550677033	0.0
30	3.416333147143177E-5	0.0	0.0	3.19355406261797	0.0
31	3.416333147143177E-5	0.0	0.0	3.2970689569764087	0.0
32	3.416333147143177E-5	0.0	0.0	3.386508558768617	0.0
33	3.416333147143177E-5	0.0	0.0	3.481482620259197	0.0
34	3.416333147143177E-5	0.0	0.0	3.579428891587792	0.0
35	3.416333147143177E-5	0.0	0.0	3.7083613045609756	0.0
36	3.416333147143177E-5	0.0	0.0	3.8120128522452994	0.0
37	3.416333147143177E-5	0.0	0.0	3.921813799594481	0.0
38	3.416333147143177E-5	0.0	0.0	4.030658173662463	0.0
39	3.416333147143177E-5	0.0	0.0	4.146608520676502	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCGGT	35	1.212793E-7	45.000004	31
GCCGAAT	20	7.035078E-4	45.0	41
TATCTCG	55	1.8189894E-12	45.0	1
ATCGGTT	25	3.8922895E-5	45.0	27
GTACGTA	40	6.8212103E-9	45.0	11
CGTTTTT	15590	0.0	43.5712	1
CGACGGT	730	0.0	43.150684	28
CGTAATA	115	0.0	43.04348	44
GCGTAAG	115	0.0	43.04348	1
CGGTCTA	750	0.0	42.3	31
GCTACGA	330	0.0	42.272728	10
ACTACGG	155	0.0	42.09677	2
TACTCGC	200	0.0	41.625	45
TGCGTAT	60	3.6379788E-12	41.250004	37
ACTCACG	55	6.184564E-11	40.909092	1
TCACGAC	795	0.0	40.75472	25
CGTAAGG	465	0.0	40.645157	2
TAATGCG	185	0.0	40.135136	1
CTAGACG	90	0.0	40.0	1
ATTAACG	180	0.0	40.0	1
>>END_MODULE