FastQCFastQC Report
Thu 26 May 2016
SRR1545053_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545053_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1596591
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74410.4660554894772675No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC59350.3717295162004546No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG19980.1251416298851741No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAC207.034112E-445.00000421
GTACCGC207.034112E-445.00000415
GTCGATC302.1660344E-645.00000436
CACCTCG406.8175723E-945.00000438
CTACGTA207.034112E-445.00000428
CGCTAAT207.034112E-445.00000415
ACTCGGT207.034112E-445.00000425
CGTACGT207.034112E-445.00000435
TTACGTA207.034112E-445.00000414
CGTAAGT207.034112E-445.00000440
TATAGCG950.045.0000041
CGGTAAG207.034112E-445.00000442
ATCGATA302.1660344E-645.00000422
TACGTAC207.034112E-445.00000429
CTTACCG207.034112E-445.00000440
TCGCCGT453.8562575E-1045.025
TCGCCCG253.89149E-545.037
TAATAGT502.1827873E-1145.035
GTCGCCC253.89149E-545.017
CGAACCC1500.045.034